Carbonic Anhydrase-1 Recombinant Protein | CA1 recombinant protein

Recombinant Human Carbonic Anhydrase-1

Cat. Num. AAA143592

• Gene Names: CA1; CAB; CA-I; Car1; HEL-S-11
• Purity: Greater than 95.0% as determined by SDS-PAGE.
• Synonyms: Carbonic Anhydrase-1; Recombinant Human Carbonic Anhydrase-1; Carbonic Anhydrase 1 Human; Carbonic Anhydrase-1 Human Recombinant; CA-1; CA1; CAI; CA-I; Carbonate dehydratase I; Carbonic anhydrase I; Carbonic anhydrase 1; Car1; CA1 recombinant protein

• Host: E Coli
• Purity/Purification: Greater than 95.0% as determined by SDS-PAGE.
• Form/Format: The Carbonic Anhydrase-1 protein solution contains 20mM Tris-HCl, pH-8, 1mM DTT and 10% Glycerol.; Sterile filtered colorless solution.
• Sequence: MGSSHHHHHH SSGLVPRGSH MASPDWGYDD KNGPEQWSKL YPIANGNNQS PVDIKTSETK HDTSLKPISV SYNPATAKEI INVGHSFHVN FEDNDNRSVL KGGPFSDSYR LFQFHFHWGS TNEHGSEHTV DGVKYSAELH VAHWNSAKYS SLAEAASKAD GLAVIGVLMK VGEANPKLQK VLDALQAIKT KGKRAPFTNF DPSTLLPSSL DFWTYPGSLT HPPLYESVTW IICKESISVS SEQLAQFRSL LSNVEGDNAV PMQHNNRPTQ PLKGRTVRAS F
• Sequence Length: 261

• Preparation and Storage: Store at 4 degree C if entire vial will be used within 2-4 weeks. Store, frozen at -20 degree C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.

Related Product Information for CA1 recombinant protein

Description: Recombinant Human Carbonic anhydrase 1 produced in E Coli is a single, non-glycosylated polypeptide chain containing 281 amino acids (1-261 a.a) and having a molecular mass of 31 kDa. Carbonic anhydrase 1 is fused to 20 amino acid His-Tag at N-terminus and purified by conventional chromatography techniques.

Introduction: Carbonic anhydrase 1 is a zinc metalloenzyme that catalyses reversible hydration of CO2 (CO2 + H2O HCO3- + H+). Carbonic anhydrase 1 is essential to many biological processes such as cellular respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, salvia, and gastric acid. Carbonic anhydrase 1 is abundant in erythrocytes and an early marker for erythroid differentiation.

Product Categories/Family for CA1 recombinant protein

Enzymes; Carbonic Anhydrase

NCBI and Uniprot Product Information

• NCBI GI #: 192447430
• NCBI GeneID: 759
• NCBI Accession #: NP_001122301.1
• NCBI GenBank Nucleotide #: NM_001128829.3
• UniProt Accession #: P00915
• Molecular Weight: 28,870 Da
• NCBI Official Full Name: carbonic anhydrase 1 isoform a
• NCBI Official Synonym Full Names: carbonic anhydrase I
• NCBI Official Symbol: CA1
• NCBI Official Synonym Symbols: CAB; CA-I; Car1; HEL-S-11
• NCBI Protein Information: carbonic anhydrase 1; carbonate dehydratase I; carbonic anhydrase B; epididymis secretory protein Li 11
• UniProt Protein Name: Carbonic anhydrase 1
• Protein Family: Cation-transporting ATPase
• UniProt Gene Name: CA1
• UniProt Synonym Gene Names: CAB; CA-I
• UniProt Entry Name: CAH1_HUMAN

NCBI Description

Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva and gastric acid. They show extensive diversity in tissue distribution and in their subcellular localization. This CA1 gene is closely linked to the CA2 and CA3 genes on chromosome 8. It encodes a cytosolic protein that is found at the highest level in erythrocytes. Allelic variants of this gene have been described in some populations. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2014]

Uniprot Description

CA1: Reversible hydration of carbon dioxide. Can hydrates cyanamide to urea. Belongs to the alpha-carbonic anhydrase family.

Protein type: Lyase; Energy Metabolism - nitrogen; EC 4.2.1.1

Chromosomal Location of Human Ortholog: 8q21.2

Cellular Component: cytosol

Molecular Function: carbonate dehydratase activity; zinc ion binding

Biological Process: bicarbonate transport; one-carbon compound metabolic process