Rabbit anti-Human CBFB Polyclonal Antibody | anti-CBFB antibody

Anti-CBFB Antibody, Rabbit Polyclonal

Cat. Num. AAA8107419

• Gene Names: CBFB; PEBP2B
• Reactivity: Human
• Applications: Western Blot, ELISA, Immunoprecipitation
• Purity: Protein A & Antigen Affinity
• Synonyms: CBFB; Polyclonal Antibody; Anti-CBFB Antibody; Rabbit Polyclonal; CBFB/CBF-beta Antibody; Rabbit PAb; Antigen Affinity Purified; core-binding factor; beta subunit; Anti-PEBP2B Antibody; anti-CBFB antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human CBFB/CBF-beta
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-CBFB antibody: Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Application Notes: WB: 1:500-1:2000; ELISA: 1:5000-1:10,000; IP: 4-6uL/mg of lysate
• Immunogen: Recombinant Human CBFB/CBF-beta protein
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with purified, recombinant Human CBFB/CBF-beta (rh CBFB/CBF-beta). CBFB/CBF-beta specific IgG was purified by Human CBFB/CBF-beta affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Immunoprecipitation (IP)

(CBFB was immunoprecipitated using:Lane A:0.5 mg Hela Whole Cell Lysate2 uL anti-CBFB rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-CBFB rabbit polyclonal antibody,at 1:200 dilution Secondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique.Performed under reducing conditions.Predicted band size: 22 kDaObserved band size: 22 kDa)

Western Blot (WB)

(Anti-CBFB rabbit polyclonal antibody at 1:500 dilutionLane A: HeLa Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:22 kDaObserved band size:22 kDa)

Related Product Information for anti-CBFB antibody

CBFB is the beta subunit of a heterodimeric core-binding transcription factor belonging to the PEBP2/CBF transcription factor family which master-regulates a host of genes specific to hematopoiesis (e.g., RUNX1) and osteogenesis (e.g., RUNX2). CBFB is a non-DNA binding regulatory subunit; it allosterically enhances DNA binding by alpha subunit as the complex binds to the core site of various enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers and GM-CSF promoters. Alternative splicing generates two mRNA variants, each encoding a distinct carboxyl terminus. In some cases, a pericentric inversion of chromosome 16 [inv(16) (p13q22)] produces a chimeric transcript consisting of the N terminus of core-binding factor beta in a fusion with the C-terminal portion of the smooth muscle myosin heavy chain 11. This chromosomal rearrangement is associated with acute myeloid leukemia of the M4Eo subtype. Two transcript variants encoding different isoforms have been found for CBFB gene. Mutations in CBFB are implicated in cases of breast cancer.

References

Hart SM. et al., 2003, Haematologica. 87(12): 1307-23. Liu P. et al., 1995, Cold Spring Harb Symp Quant Biol. 59: 547-53. Liu P. et al., 1993, Science. 261 (5124): 1041-4.

NCBI and Uniprot Product Information

• NCBI GI #: 13124873
• NCBI GeneID: 865
• NCBI Accession #: NP_001746.1
• NCBI GenBank Nucleotide #: NM_001755.2
• UniProt Accession #: Q13951; Q13124; Q9HCT2; A8K347
• Molecular Weight: 21,508 Da
• NCBI Official Full Name: core-binding factor subunit beta isoform 2
• NCBI Official Synonym Full Names: core-binding factor, beta subunit
• NCBI Official Symbol: CBFB
• NCBI Official Synonym Symbols: PEBP2B
• NCBI Protein Information: core-binding factor subunit beta; CBF-beta; PEA2-beta; PEBP2-beta; SL3-3 enhancer factor 1 beta subunit; SL3-3 enhancer factor 1 subunit beta; SL3/AKV core-binding factor beta subunit; polyomavirus enhancer-binding protein 2 beta subunit; polyomavirus enh
• UniProt Protein Name: Core-binding factor subunit beta
• Protein Family: Core-binding factor
• UniProt Gene Name: CBFB
• UniProt Synonym Gene Names: CBF-beta; PEA2-beta; PEBP2-beta
• UniProt Entry Name: PEBB_HUMAN

NCBI Description

The protein encoded by this gene is the beta subunit of a heterodimeric core-binding transcription factor belonging to the PEBP2/CBF transcription factor family which master-regulates a host of genes specific to hematopoiesis (e.g., RUNX1) and osteogenesis (e.g., RUNX2). The beta subunit is a non-DNA binding regulatory subunit; it allosterically enhances DNA binding by alpha subunit as the complex binds to the core site of various enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers and GM-CSF promoters. Alternative splicing generates two mRNA variants, each encoding a distinct carboxyl terminus. In some cases, a pericentric inversion of chromosome 16 [inv(16)(p13q22)] produces a chimeric transcript consisting of the N terminus of core-binding factor beta in a fusion with the C-terminal portion of the smooth muscle myosin heavy chain 11. This chromosomal rearrangement is associated with acute myeloid leukemia of the M4Eo subtype. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

CBFB: CBF binds to the core site, 5'-PYGPYGGT-3', of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, LCK, IL3 and GM- CSF promoters. CBFB enhances DNA binding by RUNX1. A chromosomal aberration involving CBFB is associated with acute myeloid leukemia of M4EO subtype. Pericentric inversion inv(16)(p13;q22). The inversion produces a fusion protein that consists of the 165 N-terminal residues of CBF-beta (PEPB2) with the tail region of MYH11. Belongs to the CBF-beta family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Oncoprotein; DNA-binding; Transcription factor

Chromosomal Location of Human Ortholog: 16q22.1

Cellular Component: membrane; nucleus

Molecular Function: protein binding; DNA binding; transcription coactivator activity; transcription factor activity

Biological Process: osteoblast differentiation; transcription from RNA polymerase II promoter; lymphocyte differentiation; cell maturation; myeloid cell differentiation; positive regulation of transcription from RNA polymerase II promoter