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Western Blot (WB) (All lanes : Anti-RPS7 Antibody (C-Term) at 1:2000 dilutionLane 1: Hela whole cell lysateLane 2: A549 whole cell lysateLane 3: HepG2 whole cell lysateLane 4: Jurkat whole cell lysateLane 5: NIH/3T3 whole cell lysateLane 6: PC-12 whole cell lysateLysates/proteins at 20 ug per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 22 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

Rabbit RPS7 Polyclonal Antibody | anti-RPS7 antibody

RPS7 Antibody (C-Term)

Gene Names
RPS7; S7; DBA8
Reactivity
Human, Mouse, Rat; Predicted Reactivity: Bovine, Mouse, Rat
Applications
Western Blot, ELISA
Synonyms
RPS7; Polyclonal Antibody; RPS7 Antibody (C-Term); 40S ribosomal protein S7; anti-RPS7 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse, Rat; Predicted Reactivity: Bovine, Mouse, Rat
Clonality
Polyclonal
Isotype
Ig
Specificity
This RPS7 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 158-191 amino acids from human RPS7.
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Sequence Length
194
Applicable Applications for anti-RPS7 antibody
Western Blot (WB), ELISA (EIA)
Application Notes
WB:~~1:2000
Subtitle
Purified Rabbit Polyclonal Antibody (Pab)
Cellular Location
Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Note=Colocalizes with NEK6 in the centrosome.
Antigen Type
Synthesized Peptide
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(All lanes : Anti-RPS7 Antibody (C-Term) at 1:2000 dilutionLane 1: Hela whole cell lysateLane 2: A549 whole cell lysateLane 3: HepG2 whole cell lysateLane 4: Jurkat whole cell lysateLane 5: NIH/3T3 whole cell lysateLane 6: PC-12 whole cell lysateLysates/proteins at 20 ug per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 22 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

Western Blot (WB) (All lanes : Anti-RPS7 Antibody (C-Term) at 1:2000 dilutionLane 1: Hela whole cell lysateLane 2: A549 whole cell lysateLane 3: HepG2 whole cell lysateLane 4: Jurkat whole cell lysateLane 5: NIH/3T3 whole cell lysateLane 6: PC-12 whole cell lysateLysates/proteins at 20 ug per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 22 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Related Product Information for anti-RPS7 antibody
Required for rRNA maturation.
Product Categories/Family for anti-RPS7 antibody
References
Annilo T.,et al.Gene 165:297-302 (1995). Ota T.,et al.Nat. Genet. 36:40-45 (2004). Hillier L.W.,et al.Nature 434:724-731 (2005). Mural R.J.,et al.Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases. Vladimirov S.N.,et al.Eur. J. Biochem. 239:144-149 (1996).

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
22127 Da
NCBI Official Full Name
40S ribosomal protein S7
NCBI Official Synonym Full Names
ribosomal protein S7
NCBI Official Symbol
RPS7
NCBI Official Synonym Symbols
S7; DBA8
NCBI Protein Information
40S ribosomal protein S7
UniProt Protein Name
40S ribosomal protein S7
Protein Family
UniProt Gene Name
RPS7
UniProt Entry Name
RS7_HUMAN

NCBI Description

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 40S subunit. The protein belongs to the S7E family of ribosomal proteins. It is located in the cytoplasm. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq, Jul 2008]

Uniprot Description

RPS7: Required for rRNA maturation. Defects in RPS7 are the cause of Diamond-Blackfan anemia type 8 (DBA8). DBA8 is a form of Diamond-Blackfan anemia, a congenital non-regenerative hypoplastic anemia that usually presents early in infancy. Diamond-Blackfan anemia is characterized by a moderate to severe macrocytic anemia, erythroblastopenia, and an increased risk of malignancy. 30 to 40% of Diamond-Blackfan anemia patients present with short stature and congenital anomalies, the most frequent being craniofacial (Pierre-Robin syndrome and cleft palate), thumb and urogenital anomalies. Belongs to the ribosomal protein S7e family.

Protein type: Ribosomal; Translation

Chromosomal Location of Human Ortholog: 2p25

Cellular Component: cytoplasm; cytosol; focal adhesion; membrane; microtubule organizing center; nucleolus; nucleoplasm; nucleus; ribonucleoprotein complex; ribosome; small subunit processome

Molecular Function: protein binding; RNA binding; structural constituent of ribosome

Biological Process: cell differentiation; mRNA catabolic process, nonsense-mediated decay; neural tube closure; ribosomal small subunit biogenesis and assembly; rRNA processing; SRP-dependent cotranslational protein targeting to membrane; translation; translational initiation; viral transcription

Disease: Diamond-blackfan Anemia 8

Research Articles on RPS7

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Product Notes

The RPS7 rps7 (Catalog #AAA9217233) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The RPS7 Antibody (C-Term) reacts with Human, Mouse, Rat; Predicted Reactivity: Bovine, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's RPS7 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB:~~1:2000. Researchers should empirically determine the suitability of the RPS7 rps7 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RPS7, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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