Rat Nuclear Factor, Interleukin 3 Regulated ELISA Kit | NFIL3 elisa kit

Rat Nuclear Factor, Interleukin 3 Regulated ELISA Kit

Cat. Num. AAA761199

• Gene Names: NFIL3; E4BP4; IL3BP1; NFIL3A; NF-IL3A
• Reactivity: Rat
• Synonyms: Nuclear Factor; Interleukin 3 Regulated; Rat Nuclear Factor; Interleukin 3 Regulated ELISA Kit; NFIL3/E4BP4/IL3BP1/NF-IL3A/E4BP4IL3BP1/Interleukin-3 promoter transcriptional activator/Interleukin-3-binding protein 1/NF-IL3A/NFIL3AE4 promoter-binding protein 4/nuclear factor interleukin 3 regulated protein/Transcriptional activator NF-IL3A; NFIL3 elisa kit

• Reactivity: Rat
• Sequence Length: 462

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 0.156-10ng/ml
• Sensitivity: 0.094ng/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for NFIL3 elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. anti-Rat NFIL3 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Rat NFIL3 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Rat NFIL3 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration ofRat NFIL3 can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 14198274
• NCBI GeneID: 4783
• Molecular Weight: 51,472 Da
• NCBI Official Full Name: Nuclear factor, interleukin 3 regulated
• NCBI Official Synonym Full Names: nuclear factor, interleukin 3 regulated
• NCBI Official Symbol: NFIL3
• NCBI Official Synonym Symbols: E4BP4; IL3BP1; NFIL3A; NF-IL3A
• NCBI Protein Information: nuclear factor interleukin-3-regulated protein
• UniProt Protein Name: Nuclear factor interleukin-3-regulated protein
• Protein Family: Nuclear factor interleukin-3-regulated protein
• UniProt Gene Name: NFIL3
• UniProt Synonym Gene Names: E4BP4; IL3BP1
• UniProt Entry Name: NFIL3_HUMAN

NCBI Description

The protein encoded by this gene is a transcriptional regulator that binds as a homodimer to activating transcription factor (ATF) sites in many cellular and viral promoters. The encoded protein represses PER1 and PER2 expression and therefore plays a role in the regulation of circadian rhythm. Three transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Feb 2014]

Uniprot Description

E4BP4: Acts as a transcriptional regulator that recognizes and binds to the sequence 5'-[GA]TTA[CT]GTAA[CT]-3', a sequence present in many cellular and viral promoters. Represses transcription from promoters with activating transcription factor (ATF) sites. Represses promoter activity in osteoblasts. Represses transcriptional activity of PER1. Represses transcriptional activity of PER2 via the B- site on the promoter. Activates transcription from the interleukin-3 promoter in T-cells. Competes for the same consensus-binding site with PAR DNA-binding factors (DBP, HLF and TEF). Component of the circadian clock that acts as a negative regulator for the circadian expression of PER2 oscillation in the cell-autonomous core clock. Protects pro-B cells from programmed cell death. Belongs to the bZIP family. NFIL3 subfamily.

Protein type: DNA-binding; Transcription factor

Chromosomal Location of Human Ortholog: 9q22

Cellular Component: nucleus

Molecular Function: DNA binding; protein binding; transcription corepressor activity; transcription factor activity

Biological Process: circadian rhythm; immune response; negative regulation of transcription from RNA polymerase II promoter; transcription from RNA polymerase II promoter