C1D recombinant protein

C1D Protein, Human, Recombinant (GST Tag)

Cat. Num. AAA8121165

• Gene Names: C1D; LRP1; hC1D; Rrp47; SUNCOR; SUN-CoR
• Purity: >80% as determined by SDS-PAGE
• Synonyms: C1D; C1D Protein; Human; Recombinant (GST Tag); Human C1D Protein (GST Tag); hC1D Protein; LRP1 Protein; Rrp47 Protein; SUN-CoR Protein; SUNCOR Protein; C1D nuclear receptor corepressor; C1D recombinant protein

• Host: E Coli
• Purity/Purification: >80% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.5. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Ser141

• Species: Human
• Predicted N Terminal: Met
• Tag: N-GST
• Protein Construction: A DNA sequence encoding the human C1D (Q13901)(Met 1-Ser 141) was fused with the GST tag at the N-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for C1D recombinant protein

Background: C1D nuclear receptor corepressor belongs to the C1D family. It is a DNA binding and apoptosis-inducing protein.C1D nuclear receptor corepressorinteracts with TSNAX and DNA-PKcs. It acts as a corepressor for the thyroid hormone receptor. It is thought that C1D nuclear receptor corepressor regulates TRAX/Translin complex formation. It is expressed in kidney, heart, brain, spleen, lung, testis, liver and small intestine. It plays a role in the recruitment of the RNA exosome complex to pre-rRNA to mediate the 3'-5' end processing of the 5.8S rRNA; this function may include MPHOSPH6. It potentiates transcriptional repression by NR1D1 and THRB. C1D nuclear receptor corepressorcan activate PRKDC not only in the presence of linear DNA but also in the presence of supercoiled DNA. It also can induce apoptosis in a p53/TP53 dependent manner.

Product Categories/Family for C1D recombinant protein

Apoptosis Transcription Factors and Regulators

References

Schilders G, et al. (2007) C1D is a major autoantibody target in patients with the polymyositis-scleroderma overlap syndrome. Arthritis Rheum.56(7):2449-54.Schilders G, et al. (2007) C1D and hMtr4p associate with the human exosome subunit PM/Scl-100 and are involved in pre-rRNA processing. Nucleic Acids Res. 35(8):2564-72.Erdemir T, et al. (2002) DNA damage-dependent interaction of the nuclear matrix protein C1D with Translin-associated factor X (TRAX). J Cell Sci. 115(Pt 1):207-16.

NCBI and Uniprot Product Information

• NCBI GI #: 298231253
• NCBI GeneID: 10438
• NCBI Accession #: NP_001177192.1
• NCBI GenBank Nucleotide #: NM_001190263.1
• UniProt Accession #: Q13901; Q05D64; A8K336; D6W5F8
• Molecular Weight: 16,019 Da
• NCBI Official Full Name: nuclear nucleic acid-binding protein C1D
• NCBI Official Synonym Full Names: C1D nuclear receptor corepressor
• NCBI Official Symbol: C1D
• NCBI Official Synonym Symbols: LRP1; hC1D; Rrp47; SUNCOR; SUN-CoR
• NCBI Protein Information: nuclear nucleic acid-binding protein C1D; C1D DNA-binding protein; C1D nuclear receptor co-repressor; nuclear DNA-binding protein; small unique nuclear receptor co-repressor; small unique nuclear receptor corepressor
• UniProt Protein Name: Nuclear nucleic acid-binding protein C1D
• Protein Family: Nuclear nucleic acid-binding protein
• UniProt Gene Name: C1D
• UniProt Synonym Gene Names: hC1D
• UniProt Entry Name: C1D_HUMAN

NCBI Description

The protein encoded by this gene is a DNA binding and apoptosis-inducing protein and is localized in the nucleus. It is also a Rac3-interacting protein which acts as a corepressor for the thyroid hormone receptor. This protein is thought to regulate TRAX/Translin complex formation. Alternate splicing results in multiple transcript variants that encode the same protein. Multiple pseudogenes of this gene are found on chromosome 10.[provided by RefSeq, Jun 2010]

Uniprot Description

C1D: Plays a role in the recruitment of the RNA exosome complex to pre-rRNA to mediate the 3'-5' end processing of the 5.8S rRNA; this function may include MPHOSPH6. Can activate PRKDC not only in the presence of linear DNA but also in the presence of supercoiled DNA. Can induce apoptosis in a p53/TP53 dependent manner. May regulate the TRAX/TSN complex formation. Potentiates transcriptional repression by NR1D1 and THRB. Belongs to the C1D family.

Protein type: Nuclear receptor co-regulator; Nucleolus; Transcription, coactivator/corepressor; DNA-binding

Chromosomal Location of Human Ortholog: 2p13-p12

Cellular Component: transcriptional repressor complex; cytoplasm; nucleolus; nucleus; nuclear exosome (RNase complex)

Molecular Function: ligand-dependent nuclear receptor binding; protein binding; DNA binding; RNA binding; transcription corepressor activity

Biological Process: maturation of 5.8S rRNA; transcription, DNA-dependent; apoptosis; negative regulation of transcription, DNA-dependent