Rabbit anti-Human E3 ubiquitin-protein ligase RBX1 Polyclonal Antibody | anti-RBX1 antibody

Rabbit anti-human E3 ubiquitin-protein ligase RBX1 polyclonal Antibody(RBX1)

Cat. Num. AAA1497324

• Gene Names: RBX1; ROC1; RNF75; BA554C12.1
• Reactivity: Human
• Applications: ELISA, Immunohistochemistry
• Purity: Antigen Affinity Purified
• Synonyms: E3 ubiquitin-protein ligase RBX1; Polyclonal Antibody; Rabbit anti-human E3 ubiquitin-protein ligase RBX1 polyclonal Antibody(RBX1); Protein ZYP; RING finger protein 75; RING-box protein 1; Rbx1; Regulator of cullins 1; RBX1; RNF75; ROC1; anti-RBX1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Antigen Affinity Purified
• Sequence Length: 108

• Applicable Applications for anti-RBX1 antibody: ELISA (EIA), Immunohistochemistry (IHC)
• Application Notes: Recommended dilution: IHC:1:20-1:200
• Storage Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
• Conjugate: Non-conjugated
• Immunogen: Recombinant human E3 ubiquitin-protein ligase RBX1 protein (1-108aa)
• Santa Cruz Alternative: Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-5200 / sc-5201 / sc-5202
• Preparation and Storage: Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C or -80 degree C. Avoid repeated freeze.

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human rectum using MBS1497324 at dilution 1:100)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human placenta using MBS1497324 at dilution 1:100)

Related Product Information for anti-RBX1 antibody

E3 ubiquitin ligase component of multiple cullin-RING-based E3 ubiquitin-protein ligase complexes which mediate the ubiquitination and subsequent proteasomal degradation of target proteins, including proteins involved in cell cycle progression, signal transduction, transcription and transcription-coupled nucleotide excision repair. The functional specificity of the E3 ubiquitin-protein ligase complexes depends on the variable substrate recognition components. As a component of the CSA complex promotes the ubiquitination of ERCC6 resulting in proteasomal degradation. Through the RING-type zinc finger, seems to recruit the E2 ubiquitination enzyme, like CDC34, to the complex and brings it into close proximity to the substrate. Probably also stimulates CDC34 autoubiquitination. May be required for histone H3 and histone H4 ubiquitination in response to ultraviolet and for subsequent DNA repair. Promotes the neddylation of CUL1, CUL2, CUL4 and CUL4 via its interaction with UBE2M. Involved in the ubiquitination of KEAP1, ENC1 and KLHL41. In concert with ATF2 and CUL3, promotes degradation of KAT5 thereby attenuating its ability to acetylate and activate ATM.

NCBI and Uniprot Product Information

• NCBI GI #: 7657508
• NCBI GeneID: 9978
• NCBI Accession #: NP_055063.1
• NCBI GenBank Nucleotide #: NM_014248.3
• UniProt Accession #: P62877; Q8N6Z8; Q9D1S2; Q9WUK9; Q9Y254; B2RDY1
• Molecular Weight: 12,274 Da
• NCBI Official Full Name: E3 ubiquitin-protein ligase RBX1
• NCBI Official Synonym Full Names: ring-box 1, E3 ubiquitin protein ligase
• NCBI Official Symbol: RBX1
• NCBI Official Synonym Symbols: ROC1; RNF75; BA554C12.1
• NCBI Protein Information: E3 ubiquitin-protein ligase RBX1
• UniProt Protein Name: E3 ubiquitin-protein ligase RBX1
• Protein Family: E3 ubiquitin-protein ligase
• UniProt Gene Name: RBX1
• UniProt Synonym Gene Names: RNF75; ROC1; Rbx1
• UniProt Entry Name: RBX1_HUMAN

NCBI Description

This locus encodes a RING finger-like domain-containing protein. The encoded protein interacts with cullin proteins and likely plays a role in ubiquitination processes necessary for cell cycle progression. This protein may also affect protein turnover. Related pseudogenes exist on chromosomes 2 and 5.[provided by RefSeq, Sep 2010]

Uniprot Description

RBX1: E3 ubiquitin ligase component of multiple cullin-RING- based E3 ubiquitin-protein ligase complexes which mediate the ubiquitination and subsequent proteasomal degradation of target proteins, including proteins involved in cell cycle progression, signal transduction, transcription and transcription-coupled nucleotide excision repair. The functional specificity of the E3 ubiquitin-protein ligase complexes depends on the variable substrate recognition components. As a component of the CSA complex promotes the ubiquitination of ERCC6 resulting in proteasomal degradation. Through the RING-type zinc finger, seems to recruit the E2 ubiquitination enzyme, like CDC34, to the complex and brings it into close proximity to the substrate. Probably also stimulates CDC34 autoubiquitination. May be required for histone H3 and histone H4 ubiquitination in response to ultraviolet and for subsequent DNA repair. Promotes the neddylation of CUL1, CUL2, CUL4 and CUL4 via its interaction with UBE2M. Part of a SCF complex consisting of CUL1, RBX1, SKP1 and SKP2. Part of a SCF-like complex consisting of CUL7, RBX1, SKP1 and FBXW8. Part of CBC(VHL) complexes with elongin BC complex (TCEB1 and TCEB2), CUL2 or CUL5 and VHL. Part of the CSA complex (DCX(ERCC8) complex), a DCX E3 ubiquitin-protein ligase complex containing ERCC8, RBX1, DDB1 and CUL4A; the CSA complex interacts with RNA polymerase II; upon UV irradiation it interacts with the COP9 signalosome and preferentially with the hyperphosphorylated form of RNA polymerase II. Part of multisubunit E3 ubiquitin ligase complexes with elongin BC complex (TCEB1 and TCEB2), CUL2 and MED8; elongin BC complex (TCEB1 and TCEB2), CUL5 and MUF1. Part of multisubunit complexes with elongin BC complex (TCEB1 and TCEB2), elongin A/TCEB3 or SOCS1 or WSB1 and CUL5. Interacts directly with CUL1 and probably also with CUL2, CUL3, CUL4A, CUL4B, CUL5 and CUL7. Probably interacts with CDC34. Interacts with COPS6. Component of the DCX DET1-COP1 ubiquitin ligase complex at least composed of RBX1, DET1, DDB1, CUL4A and COP1. Part of an E3 ligase complex composed of RBX1, DDB1, DDB2 and CUL4A or CUL4B. Interacts with UBE2M. Part of a SCF complex consisting of CUL1, FBXO3, RBX1 and SKP1; this complex interacts with PML via FBXO3. Interacts with human adenovirus early E1A protein; this interaction inhibits RBX1-CUL1-dependent elongation reaction of ubiquitin chains by the SCF(FBW7) complex. Component of the SCF(Cyclin F) complex consisting of CUL1, RBX1, SKP1 and CCNF. Widely expressed. Belongs to the RING-box family.

Protein type: Ligase; EC 6.3.2.-; Ubiquitin conjugating system; Ubiquitin ligase

Chromosomal Location of Human Ortholog: 22q13.2

Cellular Component: Cul2-RING ubiquitin ligase complex; Cul5-RING ubiquitin ligase complex; cullin-RING ubiquitin ligase complex; cytosol; nucleoplasm; SCF ubiquitin ligase complex; VCB complex

Molecular Function: ligase activity; NEDD8 ligase activity; protein binding; protein complex binding; ubiquitin protein ligase binding; ubiquitin-protein ligase activity; zinc ion binding

Biological Process: activation of MAPKK activity; axon guidance; bypass DNA synthesis; DNA damage response, detection of DNA damage; DNA repair; epidermal growth factor receptor signaling pathway; fibroblast growth factor receptor signaling pathway; innate immune response; insulin receptor signaling pathway; MAPKKK cascade; nerve growth factor receptor signaling pathway; Notch signaling pathway; nucleotide-excision repair; nucleotide-excision repair, DNA damage recognition; nucleotide-excision repair, DNA incision; proteasomal ubiquitin-dependent protein catabolic process; protein monoubiquitination; protein neddylation; protein ubiquitination; protein ubiquitination during ubiquitin-dependent protein catabolic process; Ras protein signal transduction; SCF-dependent proteasomal ubiquitin-dependent protein catabolic process; small GTPase mediated signal transduction; transcription-coupled nucleotide-excision repair; vascular endothelial growth factor receptor signaling pathway; viral reproduction