Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Rabbit anti-Rat Nuclear Receptor Subfamily 3 Polyclonal Antibody | anti-NR3C1 antibody
Nuclear Receptor Subfamily 3, Group C, Member 1 (NR3C1) Polyclonal Antibody
Immunohistochemistry in formalin fixed frozen section: 1:50-500
Enzyme-linked Immunosorbent Assay: 1:100-200
Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate.
5uL per well when used in enhanced chemilumescent (ECL).
Note: The quality control is specifically manufactured as the positive control.Not used for other purposes.
Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol,BPB 0.01%, NaN3 0.02%.
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Immunohistochemistry (IHC)
(DAB staining on fromalin fixed paraffin-embedded Liver tissue))
Immunohistochemistry (IHC)
(DAB staining on fromalin fixed paraffin-embedded Liver tissue))
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Rat Stomach Tissue))
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Rat Stomach Tissue))
NCBI and Uniprot Product Information
NCBI Description
glucocorticoid receptor that binds and activates hormone-dependent transcriptional enhancers [RGD, Feb 2006]
Uniprot Description
GR: transcription factor of the nuclear receptor family. Receptor for glucocorticoids (GC). Binds to glucocorticoid response elements (GRE) and modulates other transcription factors. Affects inflammatory responses, cellular proliferation and differentiation in target tissues. Three alternatively spliced isoforms have been described.
Protein type: DNA-binding; Mitochondrial; Nuclear receptor; Transcription factor
Chromosomal Location of Human Ortholog: 18p11
Cellular Component: cytoplasm; cytosol; dendritic spine; intracellular; membrane; microtubule organizing center; mitochondrion; neuron projection; nuclear speck; nucleoplasm; nucleus; postsynaptic density; protein complex; spindle
Molecular Function: chromatin binding; DNA binding; DNA binding transcription factor activity; double-stranded DNA binding; glucocorticoid receptor activity; glucocorticoid-activated RNA polymerase II transcription factor binding transcription factor activity; heat shock protein binding; hormone binding; Hsp70 protein binding; Hsp90 protein binding; protein binding; protein complex binding; protein dimerization activity; protein heterodimerization activity; protein homodimerization activity; receptor tyrosine kinase binding; sequence-specific DNA binding; steroid binding; steroid hormone binding; steroid hormone receptor activity; SUMO binding; transcription coactivator activity; transcription factor binding; zinc ion binding
Biological Process: adrenal gland development; aging; androgen metabolic process; brain development; cellular response to dexamethasone stimulus; cellular response to glucocorticoid stimulus; cellular response to magnesium ion; cellular response to steroid hormone stimulus; cellular response to transforming growth factor beta stimulus; chromatin remodeling; chromatin-mediated maintenance of transcription; circadian rhythm; covalent chromatin modification; female pregnancy; glucocorticoid mediated signaling pathway; glucocorticoid metabolic process; glucocorticoid receptor signaling pathway; lung development; maternal behavior; muscle atrophy; negative regulation of glucocorticoid mediated signaling pathway; negative regulation of synaptic plasticity; negative regulation of transcription from RNA polymerase II promoter; negative regulation of vascular permeability; positive regulation of glucocorticoid receptor signaling pathway; positive regulation of glutamate secretion; positive regulation of neuron apoptotic process; positive regulation of transcription from RNA polymerase II promoter; regulation of cell proliferation; regulation of glucocorticoid biosynthetic process; regulation of gluconeogenesis; regulation of glucose metabolic process; regulation of transcription, DNA-templated; response to activity; response to arsenic; response to calcium ion; response to corticosterone stimulus; response to dexamethasone; response to electrical stimulus; response to inactivity; response to insulin stimulus; response to mercury ion; response to radiation; steroid hormone mediated signaling; transcription, DNA-dependent
Research Articles on NR3C1
Similar Products
Product Notes
The NR3C1 nr3c1 (Catalog #AAA2032290) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Nuclear Receptor Subfamily 3, Group C, Member 1 (NR3C1) Polyclonal Antibody reacts with Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Nuclear Receptor Subfamily 3 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA). Western Blot: 1:50-400 Immunohistochemistry in formalin fixed frozen section: 1:50-500 Enzyme-linked Immunosorbent Assay: 1:100-200. Researchers should empirically determine the suitability of the NR3C1 nr3c1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Nuclear Receptor Subfamily 3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
