BMP- 4 Recombinant Protein | BMP-4 recombinant protein

BMP- 4 (monomer), 293-408aa, Human, E Coli

Cat. Num. AAA203228

• Gene Names: BMP4; ZYME; BMP2B; OFC11; BMP2B1; MCOPS6
• Applications: SDS-Page
• Purity: > 95% by SDS-PAGE
• Synonyms: BMP- 4; BMP- 4 (monomer); 293-408aa; Human; E Coli; Bone morphogenetic protein 4; BMP-2B; DVR4; Bone morphogenetic protein 4 BMP 4; BMP2B; BMP2B1; ZYME; BMP4; BMP-4 recombinant protein

• Host: E Coli
• Purity/Purification: > 95% by SDS-PAGE
• Form/Format: Liquid. In 10mM Sodium citrate buffer (pH 3.5), 10% glycerol
• Concentration: 0.5 mg/ml (determined by Bradford assay) (varies by lot)
• Sequence: MSPKHHSQRA RKKNKNCRRH SLYVDFSDVG WNDWIVAPPG YQAFYCHGDC PFPLADHLNS TNHAIVQTLV NSVNSSIPKA CCVPTELSAI SMLYLDEYDK VVLKNYQEMV VEGCGCR
• Sequence Length: 408

• Applicable Applications for BMP-4 recombinant protein: SDS-PAGE
• Antigen Species: Human
• Endotoxin Level: < 1.0 EU per 1 microgram of protein (determined by LAL method)
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for BMP-4 recombinant protein

Bone morphogenetic protein 4 (BMP-4) is a member of the bone morphogenetic protein family which belongs to the TGF-beta superfamily. This protein is a vital regulatory molecule that functions throughout bone and cartilage development, specifically tooth development, limb formation, bone induction, and fracture repair. In human embryonic development, BMP- 4 is required for the early differentiation of the embryo and establishing of a dorsal-ventral axis. And it is overexpressed in patients with fibrodysplasia ossificans progressiva. Recombinant BMP-4 was expressed as insoluble protein aggregate in E Coli and purified by conventional chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer.

Product Categories/Family for BMP-4 recombinant protein

Hormones & Cytokines

References

Moon BS., et al. (2009) Exp Mol Med. 41 (2):116-25.; Li BC., et al. (2009) J Trauma. 66 (2):450-6;

NCBI and Uniprot Product Information

• NCBI GI #: 157276593
• NCBI GeneID: 652
• NCBI Accession #: NP_001193
• NCBI GenBank Nucleotide #: NM_001202.5
• UniProt Accession #: P12644; Q9UM80
• Molecular Weight: 13.2 kDa (117 aa), confirmed by MALDI-TOF.
• NCBI Official Full Name: bone morphogenetic protein 4 isoform a preproprotein
• NCBI Official Synonym Full Names: bone morphogenetic protein 4
• NCBI Official Symbol: BMP4
• NCBI Official Synonym Symbols: ZYME; BMP2B; OFC11; BMP2B1; MCOPS6
• NCBI Protein Information: bone morphogenetic protein 4
• UniProt Protein Name: Bone morphogenetic protein 4
• UniProt Gene Name: BMP4
• UniProt Synonym Gene Names: BMP2B; DVR4; BMP-4; BMP-2B

NCBI Description

This gene encodes a secreted ligand of the TGF-beta (transforming growth factor-beta) superfamily of proteins. Ligands of this family bind various TGF-beta receptors leading to recruitment and activation of SMAD family transcription factors that regulate gene expression. The encoded preproprotein is proteolytically processed to generate each subunit of the disulfide-linked homodimer. This protein regulates heart development and adipogenesis. Mutations in this gene are associated with orofacial cleft and microphthalmia in human patients. The encoded protein may also be involved in the pathology of multiple cardiovascular diseases and human cancers. [provided by RefSeq, Jul 2016]

Uniprot Description

Induces cartilage and bone formation. Also act in mesoderm induction, tooth development, limb formation and fracture repair. Acts in concert with PTHLH/PTHRP to stimulate ductal outgrowth during embryonic mammary development and to inhibit hair follicle induction ().