BMP-4 active protein

BMP-4

Cat. Num. AAA515794

• Gene Names: BMP4; ZYME; BMP2B; OFC11; BMP2B1; MCOPS6
• Applications: Cell Assay
• Synonyms: BMP-4; ZYME; BMP2B; OFC11; BMP2B1; MCOPS6; BMP4; BMP-4 active protein

• Host: E Coli
• Form/Format: Recombinant protein stored in 1 X PBS, 10% glycerol.

• Applicable Applications for BMP-4 active protein: Cell Assay
• Species: Human
• Endotoxin Level: Less than 0.1ng/ug (IEU/ug) of BMP-4
• Preparation and Storage: Store at desiccated below -20 degree C. It is stable at 4 degree C between 2-7 days. For long term storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

SDS-Page

(Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet.)

Related Product Information for BMP-4 active protein

Human active form BMP-4 (293-408) was expressed in E Coli cells and purified by proprietary chromatographic techniques. The protein accession number is NP_001193.2.
BMP4 is a member of the bone morphogenetic protein family that is part of the transforming growth factor-beta superfamily. BMP4 plays an important role in the onset of endochondral bone formation in humans and a reduction in expression has been associated with a variety of bone diseases, including the heritable disorder Fibrodysplasia Ossificans Progressiva (1). BMP4 acts as a novel, noncytotoxic therapeutic effector that is used to prevent growth and recurrence of glioblastomas in humans (2).

Product Categories/Family for BMP-4 active protein

Growth Factors; AKT/PKB Pathway; Cancer; ERK/MAPK Pathway; Inflammation; Invasion/Metastasis; JNK/SAPK Pathway; NfkB Pathway; p38 Pathway; PKA/PKC Pathway; WNT Signaling

References

1. Kan, L., et.al: Transgenic mice overexpressing BMP4 develop a fibrodysplasia ossificans progressiva (FOP)-like phenotype. Am. J. Path. 165: 1107-1115, 2004.
2. Piccirillo, S. G. M. et.al: Bone morphogenetic proteins inhibit the tumorigenic potential of human brain tumour-initiating cells. Nature 444: 761-765, 2006.

NCBI and Uniprot Product Information

• NCBI GI #: 157276593
• NCBI GeneID: 652
• NCBI Accession #: NP_001193.2
• NCBI GenBank Nucleotide #: NM_001202.3
• UniProt Accession #: P12644; Q9UM80
• Molecular Weight: 46,555 Da
• NCBI Official Full Name: bone morphogenetic protein 4 preproprotein
• NCBI Official Synonym Full Names: bone morphogenetic protein 4
• NCBI Official Symbol: BMP4
• NCBI Official Synonym Symbols: ZYME; BMP2B; OFC11; BMP2B1; MCOPS6
• NCBI Protein Information: bone morphogenetic protein 4; BMP-4; BMP-2B; bone morphogenetic protein 2B
• UniProt Protein Name: Bone morphogenetic protein 4
• UniProt Gene Name: BMP4
• UniProt Synonym Gene Names: BMP2B; DVR4; BMP-4; BMP-2B
• UniProt Entry Name: BMP4_HUMAN

NCBI Description

The protein encoded by this gene is a member of the bone morphogenetic protein family which is part of the transforming growth factor-beta superfamily. The superfamily includes large families of growth and differentiation factors. Bone morphogenetic proteins were originally identified by an ability of demineralized bone extract to induce endochondral osteogenesis in vivo in an extraskeletal site. This particular family member plays an important role in the onset of endochondral bone formation in humans, and a reduction in expression has been associated with a variety of bone diseases, including the heritable disorder Fibrodysplasia Ossificans Progressiva. Alternative splicing in the 5' untranslated region of this gene has been described and three variants are described, all encoding an identical protein. [provided by RefSeq, Jul 2008]

Uniprot Description

BMP4: Induces cartilage and bone formation. Also act in mesoderm induction, tooth development, limb formation and fracture repair. Acts in concert with PTHLH/PTHRP to stimulate ductal outgrowth during embryonic mammary development and to inhibit hair follicle induction. Homodimer; disulfide-linked. Interacts with GREM2. Part of a complex consisting of TWSG1 and CHRD. Interacts with the serine proteases, HTRA1 and HTRA3; the interaction with either inhibits BMP4-mediated signaling. The HTRA protease activity is required for this inhibition. Interacts with SOSTDC1. Expressed in the lung and lower levels seen in the kidney. Present also in normal and neoplastic prostate tissues, and prostate cancer cell lines. Belongs to the TGF-beta family.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 14q22-q23

Cellular Component: proteinaceous extracellular matrix; extracellular space; extracellular region

Molecular Function: heparin binding; protein binding; growth factor activity; cytokine activity; transforming growth factor beta receptor binding; chemoattractant activity

Biological Process: negative regulation of MAP kinase activity; extracellular matrix organization and biogenesis; renal system process; macrophage differentiation; activation of MAPKK activity; positive regulation of apoptosis; positive regulation of transcription, DNA-dependent; negative regulation of chondrocyte differentiation; lymphoid progenitor cell differentiation; telencephalon regionalization; germ cell development; post-embryonic development; regulation of protein import into nucleus; BMP signaling pathway; positive regulation of endothelial cell differentiation; positive chemotaxis; erythrocyte differentiation; chondrocyte differentiation; mesonephros development; kidney development; regulation of odontogenesis of dentine-containing teeth; endochondral ossification; positive regulation of cardiac muscle fiber development; negative regulation of immature T cell proliferation in the thymus; specification of organ position; monocyte differentiation; neuron fate commitment; embryonic cranial skeleton morphogenesis; negative regulation of striated muscle development; branching morphogenesis of a tube; positive regulation of epidermal cell differentiation; negative regulation of mitosis; negative regulation of phosphorylation; hemopoietic progenitor cell differentiation; steroid hormone mediated signaling; positive regulation of endothelial cell proliferation; positive regulation of transcription from RNA polymerase II promoter; embryonic digit morphogenesis; negative regulation of transcription, DNA-dependent; alveolus development; positive regulation of epithelial cell proliferation; negative regulation of apoptosis; positive regulation of protein binding; positive regulation of smooth muscle cell proliferation; positive regulation of collagen biosynthetic process; cloacal septation; negative regulation of transcription from RNA polymerase II promoter; embryonic hindlimb morphogenesis; negative regulation of cell cycle; odontogenesis; negative regulation of cell proliferation; smooth muscle development; inner ear receptor cell differentiation; ureteric bud development; intermediate mesodermal cell differentiation; regulation of smooth muscle cell differentiation; positive regulation of BMP signaling pathway; dorsoventral neural tube patterning; negative regulation of epithelial cell proliferation; smoothened signaling pathway; common-partner SMAD protein phosphorylation; negative regulation of T cell differentiation in the thymus; positive regulation of bone mineralization; positive regulation of ossification; odontogenesis of dentine-containing teeth; osteoblast differentiation; positive regulation of osteoblast differentiation; blood vessel endothelial cell proliferation during sprouting angiogenesis; telencephalon development; pituitary gland development; ureteric bud branching; regulation of cell fate commitment; neural tube closure; positive regulation of protein amino acid phosphorylation; negative regulation of myoblast differentiation; positive regulation of neuron differentiation; mesodermal cell fate determination; anterior/posterior axis specification

Disease: Orofacial Cleft 11; Microphthalmia, Syndromic 6