Bone Morphogenetic protein-4 Recombinant Protein | BMP-4 recombinant protein

Human Bone Morphogenetic protein-4 Recombinant

Cat. Num. AAA553130

• Purity: >95%, as determined by SDS-PAGE and HPLC
• Synonyms: Bone Morphogenetic protein-4; Human Bone Morphogenetic protein-4 Recombinant; Bone Morphogenetic protein ; bmp-4; bmp4; BMP-4 recombinant protein

• Host: E Coli
• Purity/Purification: >95%, as determined by SDS-PAGE and HPLC
• Form/Format: Recombinant BMP-4 was lyophilized from a 0.2 um filtered 20mM Na2CO3, pH 9.
• Sequence: MIPGNRMLMV VLLCQVLLGG ASHASLIPET GKKKVAEIQG HAGGRRSGQS HELLRDFEAT LLQMFGLRRR PQPSKSAVIP DYMRDLYRLQ SGEEEEEQIH STGLEYPERP ASRANTVRSF HHEEHLENIP GTSENSAFRF LFNLSSIPEN EVISSAELRL FREQVDQGPD WERGFHRINI YEVMKPPAEV VPGHLITRLL DTRLVHHNVT RWETFDVSPA VLRWTREKQP NYGLAIEVTH LHQTRTHQGQ HVRISRSLPQ GSGNWAQLRP LLVTFGHDGR GHALTRRRRA KRSPKHHSQR ARKKNKNCRR HSLYVDFSDV GWNDWIVAPP GYQAFYCHGD CPFPLADHLN STNHAIVQTL VNSVNSSIPK ACCVPTELSA ISMLYLDEYD KVVLKNYQEM VVEGCGCR
• Sequence Length: 408

• Domain: TGFB
• Host Note: Optimized DNA sequence encoding Human Bone Morphogenetic protein-4 mature chain was expressed in Escherichia Coli.
• Endotoxin: Endotoxin content was assayed using a LAL gel clot method.; Endotoxin level was found to be less than 0.1 ng/ug (1EU/ug).
• Reconstitution: A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
• Molecular Weight Note: Native human Bone Morphogenetic protein-4 is generated by the proteolytic removal of the signal peptide and propeptide, the molecule has a calculated molecular mass of approximately 13 kDa. Recombinant BMP-4 is a disulfide-linked monomeric protein consisting of 117 amino acid residue subunits, and migrates as an approximately as a 13 kDa protein under reducing conditions in SDS-PAGE.
• Preparation and Storage: The lyophilized protein is stable for at least 2 years from date of receipt at -20 degree C.; Upon reconstitution, this cytokine can be stored in working aliquots at 2 degree - 8 degree C for one month, or at -20 degree C for six months, with a carrier protein without detectable loss of activity. ; ; Avoid repeated freeze/thaw cycles.

Related Product Information for BMP-4 recombinant protein

BMPs are proteins that act to induce the differentiation of mesenchymal-type cells into chondrocytes and osteoblasts before initiating bone formation. They promote the differentiation of cartilage-forming cells and bone-forming cells near sites of fractures but also at ectopic locations. Some of the proteins induce the synthesis of alkaline phosphatase and collagen in osteoblasts. Some BMPs act directly on osteoblasts and promote their maturation while at the same time suppressing myogenous differentiation. Other BMPs promote the conversion of typical fibroblasts into chondrocytes and are capable also of inducing the expression of an osteoblast phenotype in non-osteogenic cell types. Intracellular signaling following engagement of receptors for some BMP proteins has been shown to involve the action of SMAD proteins. BMP4 and BMP7 have been shown to be involved also in the differentiation of sympathetic neurons. They enhance the formation of adrenergic sympathetic neurons in cultures of neural crest cells.

Product Categories/Family for BMP-4 recombinant protein

Cytokines

References

• smad, pi3k/akt, and wnt-dependent signaling pathways are involved in BMP-4-induced esc self-renewal .Stem Cells, Aug 2009; 27: 1858 - 1868.
• molecular interaction of BMP-4, tgf-β, and estrogens in lactotrophs: impact on the prl promoter .Mol. Endocrinol., Jul 2009; 23: 1102 - 1114.
• suppression of vegf expression and lesion size in laser-induced cnv in a mouse model overexpressing BMP-4 .Invest. Ophthalmol. Vis. Sci., Apr 2009; 50: 1193.
• altered shear stress stimulates upregulation of endothelial vcam-1 and icam-1 in a BMP-4– and tgf-β1–dependent pathway .Arterioscler Thromb Vasc Biol, Feb 2009; 29: 254 - 260.
• the bone morphogenetic protein (bmp) -4 is involved in the regulation of human megakaryocytic differentiation during thrombopoietin signaling. .Blood (ASH Annual Meeting Abstracts), Nov 2008; 112: 1339.