Rabbit BMP4 Polyclonal Antibody | anti-BMP4 antibody

BMP4 antibody

Cat. Num. AAA9401115

• Gene Names: BMP4; ZYME; BMP2B; OFC11; BMP2B1; MCOPS6
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: Antibodies were purified by affinity purification using immunogen.
• Synonyms: BMP4; Polyclonal Antibody; BMP4 antibody; BMP2B; BMP2B1; MCOPS6; ZYME; anti-BMP4 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Specificity: The antibody detects endogenous level of total BMP4 antibody.
• Purity/Purification: Antibodies were purified by affinity purification using immunogen.
• Form/Format: Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1.0 mg/ml (varies by lot)
• Sequence Length: 408

• Applicable Applications for anti-BMP4 antibody: Western Blot (WB)
• Application Notes: Western blotting: 1:500 - 1:2000
• Immunogen Type: Recombinant Protein
• Immunogen Description: Recombinant protein of human BMP4.
• Target Name: BMP4
• Preparation and Storage: Store at -20 degree C

Western Blot (WB)

(Western blot analysis of extracts of various cell lines, using BMP4 antibody.)

Related Product Information for anti-BMP4 antibody

Bone morphogenetic proteins (BMPs) were first identified as molecules that can induce ectopic bone and cartilage formation (1,2). BMPs belongs to the TGF-b superfamily, playing many diverse functions during development (3). BMPs are synthesized as precursor proteins and then processed by cleavage to release the c-terminal mature BMP. BMPs initiate signaling by binding to a receptor complex containing type I and type II serine/threonine receptor kinases that then phosphorylate Smad (mainly Smad1, 5 and 8), resulting the translocation of Smad into the nucleus. BMP was also reported to activate MAPK pathways in some systems (3,4). BMP4 plays an essential role during development. Mouse embyros deficient in BMP4 die around the time of gastrulation (5). BMP4 regulates stem cell maintenance and differentiation in various systems (6-8).

Product Categories/Family for anti-BMP4 antibody

Total protein Ab

NCBI and Uniprot Product Information

• NCBI GI #: 157276593
• NCBI GeneID: 652
• NCBI Accession #: NP_001193.2
• NCBI GenBank Nucleotide #: NM_001202.3
• UniProt Accession #: P12644; Q9UM80
• Molecular Weight: 47kd by SDS-PAGE
• NCBI Official Full Name: bone morphogenetic protein 4 preproprotein
• NCBI Official Synonym Full Names: bone morphogenetic protein 4
• NCBI Official Symbol: BMP4
• NCBI Official Synonym Symbols: ZYME; BMP2B; OFC11; BMP2B1; MCOPS6
• NCBI Protein Information: bone morphogenetic protein 4
• UniProt Protein Name: Bone morphogenetic protein 4
• Protein Family: Bone morphogenetic protein
• UniProt Gene Name: BMP4
• UniProt Synonym Gene Names: BMP2B; DVR4; BMP-4; BMP-2B
• UniProt Entry Name: BMP4_HUMAN

NCBI Description

The protein encoded by this gene is a member of the bone morphogenetic protein family which is part of the transforming growth factor-beta superfamily. The superfamily includes large families of growth and differentiation factors. Bone morphogenetic proteins were originally identified by an ability of demineralized bone extract to induce endochondral osteogenesis in vivo in an extraskeletal site. This particular family member plays an important role in the onset of endochondral bone formation in humans, and a reduction in expression has been associated with a variety of bone diseases, including the heritable disorder Fibrodysplasia Ossificans Progressiva. Alternative splicing in the 5' untranslated region of this gene has been described and three variants are described, all encoding an identical protein. [provided by RefSeq, Jul 2008]

Uniprot Description

BMP4: Induces cartilage and bone formation. Also act in mesoderm induction, tooth development, limb formation and fracture repair. Acts in concert with PTHLH/PTHRP to stimulate ductal outgrowth during embryonic mammary development and to inhibit hair follicle induction. Homodimer; disulfide-linked. Interacts with GREM2. Part of a complex consisting of TWSG1 and CHRD. Interacts with the serine proteases, HTRA1 and HTRA3; the interaction with either inhibits BMP4-mediated signaling. The HTRA protease activity is required for this inhibition. Interacts with SOSTDC1. Expressed in the lung and lower levels seen in the kidney. Present also in normal and neoplastic prostate tissues, and prostate cancer cell lines. Belongs to the TGF-beta family.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 14q22-q23

Cellular Component: extracellular space; proteinaceous extracellular matrix; extracellular region

Molecular Function: heparin binding; protein binding; growth factor activity; cytokine activity; transforming growth factor beta receptor binding; chemoattractant activity

Biological Process: negative regulation of MAP kinase activity; renal system process; extracellular matrix organization and biogenesis; macrophage differentiation; activation of MAPKK activity; positive regulation of apoptosis; positive regulation of transcription, DNA-dependent; negative regulation of chondrocyte differentiation; lymphoid progenitor cell differentiation; telencephalon regionalization; germ cell development; post-embryonic development; BMP signaling pathway; regulation of protein import into nucleus; positive regulation of endothelial cell differentiation; positive chemotaxis; erythrocyte differentiation; chondrocyte differentiation; mesonephros development; kidney development; regulation of odontogenesis of dentine-containing teeth; endochondral ossification; negative regulation of immature T cell proliferation in the thymus; positive regulation of cardiac muscle fiber development; specification of organ position; monocyte differentiation; neuron fate commitment; embryonic cranial skeleton morphogenesis; negative regulation of striated muscle development; branching morphogenesis of a tube; positive regulation of epidermal cell differentiation; negative regulation of mitosis; negative regulation of phosphorylation; positive regulation of transcription from RNA polymerase II promoter; positive regulation of endothelial cell proliferation; hemopoietic progenitor cell differentiation; steroid hormone mediated signaling; embryonic digit morphogenesis; negative regulation of transcription, DNA-dependent; alveolus development; positive regulation of epithelial cell proliferation; negative regulation of apoptosis; positive regulation of protein binding; positive regulation of smooth muscle cell proliferation; cloacal septation; positive regulation of collagen biosynthetic process; negative regulation of transcription from RNA polymerase II promoter; embryonic hindlimb morphogenesis; negative regulation of cell cycle; odontogenesis; smooth muscle development; negative regulation of cell proliferation; inner ear receptor cell differentiation; ureteric bud development; intermediate mesodermal cell differentiation; regulation of smooth muscle cell differentiation; dorsoventral neural tube patterning; positive regulation of BMP signaling pathway; negative regulation of epithelial cell proliferation; smoothened signaling pathway; common-partner SMAD protein phosphorylation; negative regulation of T cell differentiation in the thymus; positive regulation of bone mineralization; positive regulation of ossification; odontogenesis of dentine-containing teeth; osteoblast differentiation; positive regulation of osteoblast differentiation; blood vessel endothelial cell proliferation during sprouting angiogenesis; telencephalon development; ureteric bud branching; pituitary gland development; neural tube closure; regulation of cell fate commitment; positive regulation of protein amino acid phosphorylation; positive regulation of neuron differentiation; negative regulation of myoblast differentiation; mesodermal cell fate determination; anterior/posterior axis specification

Disease: Orofacial Cleft 11; Microphthalmia, Syndromic 6