Human Biliverdin Reductase A ELISA Kit | BLVRA elisa kit

Human Biliverdin Reductase A ELISA Kit

Cat. Num. AAA764719

• Gene Names: BLVRA; BVR; BLVR; BVRA
• Reactivity: Human
• Synonyms: Biliverdin Reductase A; Human Biliverdin Reductase A ELISA Kit; BLVRA/BLVRA/BVR/BVRA/biliverdin reductase A/Biliverdin-IX alpha-reductase/BLVR/BVR/BVR A/BVRA/EC 1.3.1.24; BLVRA elisa kit

• Reactivity: Human
• Sequence Length: 296

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 0.313-20ng/ml
• Sensitivity: 0.188ng/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for BLVRA elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. anti-Human BLVRA antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Human BLVRA antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human BLVRA amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Human BLVRA can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 359465572
• NCBI GeneID: 644
• NCBI Accession #: NP_001240752.1
• NCBI GenBank Nucleotide #: NM_001253823.1
• Molecular Weight: 33,428 Da
• NCBI Official Full Name: biliverdin reductase A
• NCBI Official Synonym Full Names: biliverdin reductase A
• NCBI Official Symbol: BLVRA
• NCBI Official Synonym Symbols: BVR; BLVR; BVRA
• NCBI Protein Information: biliverdin reductase A
• UniProt Protein Name: Biliverdin reductase A
• Protein Family: Biliverdin reductase
• UniProt Gene Name: BLVRA
• UniProt Synonym Gene Names: BLVR; BVR; BVR A
• UniProt Entry Name: BIEA_HUMAN

NCBI Description

The protein encoded by this gene belongs to the biliverdin reductase family, members of which catalyze the conversion of biliverdin to bilirubin in the presence of NADPH or NADH. Mutations in this gene are associated with hyperbiliverdinemia. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Dec 2011]

Uniprot Description

BVR: Reduces the gamma-methene bridge of the open tetrapyrrole, biliverdin IX alpha, to bilirubin with the concomitant oxidation of a NADH or NADPH cofactor. Monomer. Liver. Belongs to the Gfo/Idh/MocA family. Biliverdin reductase subfamily.

Protein type: Kinase, protein; Oxidoreductase; Cofactor and Vitamin Metabolism - porphyrin and chlorophyll; EC 1.3.1.24; Protein kinase, Ser/Thr (non-receptor); ATYPICAL group; BLVRA family

Chromosomal Location of Human Ortholog: 7p13

Cellular Component: cytosol

Molecular Function: biliverdin reductase activity; protein binding; zinc ion binding

Biological Process: heme catabolic process; porphyrin metabolic process

Disease: Hyperbiliverdinemia