Typical Testing Data/Standard Curve (for reference only)
Rat Biliverdin Reductase A (BLVRA) ELISA Kit | BLVRA elisa kit
Rat Biliverdin Reductase A (BLVRA) ELISA Kit
Gene Names
BLVRA; BVR; BLVR; BVRA
Reactivity
Rat
Synonyms
Biliverdin Reductase A (BLVRA); Rat Biliverdin Reductase A (BLVRA) ELISA Kit; BLVR; BVR; Biliverdin-IX alpha-reductase; BLVRA elisa kit
Reactivity
Rat
Specificity
This assay has high sensitivity and excellent specificity for detection of Biliverdin Reductase A (BLVRA).
No significant cross-reactivity or interference between Biliverdin Reductase A (BLVRA) and analogues was observed.
No significant cross-reactivity or interference between Biliverdin Reductase A (BLVRA) and analogues was observed.
Sequence Length
296
Assay Type
Double-antibody Sandwich
Samples
Tissue homogenates and Other Biological Fluids
Detection Range
0.625-40ng/mL
Sensitivity
< 0.231ng/mL
Application
Enzyme-linked immunosorbent assay for Antigen Detection.
Intra-assay Precision (Precision within an assay)
3 samples with low, middle and high level Biliverdin Reductase A (BLVRA) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays)
3 samples with low, middle and high level Biliverdin Reductase A (BLVRA) were tested on 3 different plates, 8 replicates in each plate.
CV(%) =
SD/meanX100
Intra-Assay
CV<10%
Inter-Assay
CV<12%
Preparation and Storage
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for BLVRA elisa kit
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Biliverdin Reductase A (BLVRA). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Biliverdin Reductase A (BLVRA). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Biliverdin Reductase A (BLVRA), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Biliverdin Reductase A (BLVRA) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Product Categories/Family for BLVRA elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
33,428 Da
NCBI Official Full Name
biliverdin reductase A
NCBI Official Synonym Full Names
biliverdin reductase A
NCBI Official Symbol
BLVRA
NCBI Official Synonym Symbols
BVR; BLVR; BVRA
NCBI Protein Information
biliverdin reductase A
UniProt Protein Name
Biliverdin reductase A
Protein Family
UniProt Gene Name
BLVRA
UniProt Synonym Gene Names
BLVR; BVR; BVR A
UniProt Entry Name
BIEA_HUMAN
NCBI Description
The protein encoded by this gene belongs to the biliverdin reductase family, members of which catalyze the conversion of biliverdin to bilirubin in the presence of NADPH or NADH. Mutations in this gene are associated with hyperbiliverdinemia. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Dec 2011]
Uniprot Description
BVR: Reduces the gamma-methene bridge of the open tetrapyrrole, biliverdin IX alpha, to bilirubin with the concomitant oxidation of a NADH or NADPH cofactor. Monomer. Liver. Belongs to the Gfo/Idh/MocA family. Biliverdin reductase subfamily.
Protein type: Kinase, protein; Cofactor and Vitamin Metabolism - porphyrin and chlorophyll; EC 1.3.1.24; Oxidoreductase; Protein kinase, Ser/Thr (non-receptor); ATYPICAL group; BLVRA family
Chromosomal Location of Human Ortholog: 7p13
Cellular Component: cytosol
Molecular Function: zinc ion binding; biliverdin reductase activity
Biological Process: heme catabolic process; porphyrin metabolic process
Disease: Hyperbiliverdinemia
Research Articles on BLVRA
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Product Notes
The Rat BLVRA blvra (Catalog #AAA2023621) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2023621 ELISA Kit recognizes Rat BLVRA. It is sometimes possible for the material contained within the vial of "Biliverdin Reductase A (BLVRA), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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