SFRS1 blocking peptide

SFRS1 Peptide - middle region

Cat. Num. AAA3230383

• Gene Names: SRSF1; ASF; SF2; SFRS1; SF2p33; SRp30a
• Applications: Western Blot
• Synonyms: SFRS1; SFRS1 Peptide - middle region; SFRS1 blocking peptide

• Form/Format: Lyophilized powder
• Sequence Length: 248

• Applicable Applications for SFRS1 blocking peptide: Western Blot (WB)
• Preparation and Storage: Add 100ul of sterile PBS. Final peptide concentration is 1 mg/ml in PBS. For longer periods of storage, store at -20 degree C. Avoid repeat freeze-thaw cycles.

Related Product Information for SFRS1 blocking peptide

SFRS1 is a member of the arginine/serine-rich splicing factor protein family, and functions in both constitutive and alternative pre-mRNA splicing. The protein binds to pre-mRNA transcripts and components of the spliceosome, and can either activate or repress splicing depending on the location of the pre-mRNA binding site. The protein's ability to activate splicing is regulated by phosphorylation and interactions with other splicing factor associated proteins. Multiple transcript variants encoding different isoforms have been found for this gene.Alternative mRNA splicing plays an important role in development and differentiation; many transcripts are spliced differently in distinct cell types and tissues. Both constitutive and alternative splicing occurs on spliceosomes, which are complex particles composed of small nuclear ribonucleoproteins (snRNPs) and non-snRNP proteins. The SR family of non-snRNP splicing factors is characterized by the presence of an RNA recognition motif and a serine- and arginine-rich (SR) domain. SR proteins are required at early stages of spliceosome assembly, have distinct but overlapping specificities for different pre-mRNAs, and can alter splice site choice, suggesting that they may be involved in the regulation of alternative splicing in vivo. Two of the SR proteins, ASF/SF2 (SFRS1) and SC35 (SFRS2; MIM 600813), have been extensively characterized (Bermingham et al., 1995).[supplied by OMIM].

Product Categories/Family for SFRS1 blocking peptide

Peptide

NCBI and Uniprot Product Information

• NCBI GI #: 5902076
• NCBI GeneID: 6426
• NCBI Accession #: NP_008855
• NCBI GenBank Nucleotide #: NM_006924
• UniProt Accession #: Q07955
• Molecular Weight: 27kDa
• NCBI Official Full Name: serine/arginine-rich splicing factor 1 isoform 1
• NCBI Official Synonym Full Names: serine and arginine rich splicing factor 1
• NCBI Official Symbol: SRSF1
• NCBI Official Synonym Symbols: ASF; SF2; SFRS1; SF2p33; SRp30a
• NCBI Protein Information: serine/arginine-rich splicing factor 1
• UniProt Protein Name: Serine/arginine-rich splicing factor 1
• UniProt Gene Name: SRSF1
• UniProt Synonym Gene Names: ASF; SF2; SF2P33; SFRS1; ASF-1
• UniProt Entry Name: SRSF1_HUMAN

NCBI Description

This gene encodes a member of the arginine/serine-rich splicing factor protein family. The encoded protein can either activate or repress splicing, depending on its phosphorylation state and its interaction partners. Multiple transcript variants have been found for this gene. There is a pseudogene of this gene on chromosome 13. [provided by RefSeq, Jun 2014]

Uniprot Description

SF2: a serine-arginine-rich splicing regulatory protein. Plays a role in preventing exon skipping, ensuring the accuracy of splicing and regulating alternative splicing. Interacts with other spliceosomal components, via the RS domains, to form a bridge between the 5' and 3' splice site binding components, U1 snRNP and U2AF. Can stimulate binding of U1 snRNP to a 5'-splice-site-containing pre-mRNA. Extensively phosphorylated on serine residues in the serine-arginine rich region. Three splice variant isoforms have been described. ASF/SF2 splicing enhancer (ASE) which can specifically activate ASE-dependent splicing. Isoform ASF-2 and isoform ASF-3 act as splicing repressors.

Protein type: RNA-binding; RNA splicing; Spliceosome

Chromosomal Location of Human Ortholog: 17q22

Cellular Component: nucleoplasm; cytoplasm; nuclear speck; nucleus

Molecular Function: mRNA binding; protein binding; RS domain binding; RNA binding; nucleotide binding

Biological Process: transcription from RNA polymerase II promoter; in utero embryonic development; RNA splicing; regulation of mRNA stability; regulation of translation; nuclear mRNA splicing, via spliceosome; nuclear mRNA 5'-splice site recognition; mRNA splice site selection; regulation of nuclear mRNA splicing, via spliceosome; mRNA export from nucleus; regulation of transcription, DNA-dependent; gene expression; mRNA 3'-end processing; mRNA processing; termination of RNA polymerase II transcription; cardiac muscle contraction