Human Serine/Arginine Rich Splicing Factor 1 ELISA Kit | SRSF1 elisa kit

Human Serine/Arginine Rich Splicing Factor 1 ELISA Kit

Cat. Num. AAA3804463

• Gene Names: SRSF1; ASF; SF2; SFRS1; SF2p33; SRp30a
• Reactivity: Human
• Synonyms: Serine/Arginine Rich Splicing Factor 1; Human Serine/Arginine Rich Splicing Factor 1 ELISA Kit; SRSF1 elisa kit

• Reactivity: Human
• Sequence Length: 248

• Samples: Serum, plasma, cell culture supernate and other biological fluids
• Assay Type: Sandwich
• Detection Range: 1.25ng/ml-20ng/ml
• Sensitivity: 0.1 ng/ml

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for SRSF1 elisa kit

Intended Use: The Stop Solution changes the color from blue to yellow and the intensity of the color is measured at 450 nm using a spectrophotometer. In order to measure the concentration ofSERINE; ARGININE RICH SPLICING FACTOR 1in the sample, This SERINE; ARGININE RICH SPLICING FACTOR 1ELISA Kit includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of Optical Density versusSERINE; ARGININE RICH SPLICING FACTOR 1concentration. The concentration ofSERINE; ARGININE RICH SPLICING FACTOR 1in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 5902076
• NCBI GeneID: 6426
• NCBI GenBank Nucleotide #: NP_008855.1
• UniProt Accession #: Q07955
• NCBI Official Full Name: serine/arginine-rich splicing factor 1 isoform 1
• NCBI Official Synonym Full Names: serine and arginine rich splicing factor 1
• NCBI Official Symbol: SRSF1
• NCBI Official Synonym Symbols: ASF; SF2; SFRS1; SF2p33; SRp30a
• NCBI Protein Information: serine/arginine-rich splicing factor 1
• UniProt Protein Name: Serine/arginine-rich splicing factor 1
• Protein Family: Serine/arginine-rich splicing factor
• UniProt Gene Name: SRSF1
• UniProt Synonym Gene Names: ASF; SF2; SF2P33; SFRS1; ASF-1
• UniProt Entry Name: SRSF1_HUMAN

NCBI Description

This gene encodes a member of the arginine/serine-rich splicing factor protein family. The encoded protein can either activate or repress splicing, depending on its phosphorylation state and its interaction partners. Multiple transcript variants have been found for this gene. There is a pseudogene of this gene on chromosome 13. [provided by RefSeq, Jun 2014]

Uniprot Description

SF2: a serine-arginine-rich splicing regulatory protein. Plays a role in preventing exon skipping, ensuring the accuracy of splicing and regulating alternative splicing. Interacts with other spliceosomal components, via the RS domains, to form a bridge between the 5' and 3' splice site binding components, U1 snRNP and U2AF. Can stimulate binding of U1 snRNP to a 5'-splice-site-containing pre-mRNA. Extensively phosphorylated on serine residues in the serine-arginine rich region. Three splice variant isoforms have been described. ASF/SF2 splicing enhancer (ASE) which can specifically activate ASE-dependent splicing. Isoform ASF-2 and isoform ASF-3 act as splicing repressors.

Protein type: RNA-binding; RNA splicing; Spliceosome

Chromosomal Location of Human Ortholog: 17q22

Cellular Component: nucleoplasm; cytoplasm; nuclear speck; nucleus

Molecular Function: mRNA binding; protein binding; RS domain binding; RNA binding; nucleotide binding

Biological Process: transcription from RNA polymerase II promoter; in utero embryonic development; RNA splicing; regulation of mRNA stability; regulation of translation; nuclear mRNA splicing, via spliceosome; nuclear mRNA 5'-splice site recognition; mRNA splice site selection; regulation of nuclear mRNA splicing, via spliceosome; mRNA export from nucleus; regulation of transcription, DNA-dependent; gene expression; mRNA 3'-end processing; mRNA processing; termination of RNA polymerase II transcription; cardiac muscle contraction