SFRS1 recombinant protein

SFRS1, 1-248aa, Human, His tag, E Coli (Denatured)

Cat. Num. AAA205229

• Gene Names: SRSF1; ASF; SF2; SFRS1; SF2p33; SRp30a
• Applications: SDS-Page
• Purity: > 90% by SDS-PAGE
• Synonyms: SFRS1; 1-248aa; Human; His tag; E Coli (Denatured); Serine/arginine-rich splicing factor 1; ASF; MGC5228; SF2; SF2p33; SRp30a; SFRS1 recombinant protein

• Host: E Coli
• Purity/Purification: > 90% by SDS-PAGE
• Form/Format: Liquid. In 20mM Tris-HCl Buffer (pH 8.0) containing 0.4M urea, 10% glycerol
• Concentration: 1 mg/ml (determined by Bradford assay) (varies by lot)
• Sequence: MGSSHHHHHH SSGLVPRGSH MSGGGVIRGP AGNNDCRIYV GNLPPDIRTK DIEDVFYKYG AIRDIDLKNR RGGPPFAFVE FEDPRDAEDA VYGRDGYDYD GYRLRVEFPR SGRGTGRGGG GGGGGGAPRG RYGPPSRRSE NRVVVSGLPP SGSWQDLKDH MREAGDVCYA DVYRDGTGVV EFVRKEDMTY AVRKLDNTKF RSHEGETAYI RVKVDGPRSP SYGRSRSRSR SRSRSRSRSN SRSRSYSPRR SRGSPRYSPR HSRSRSRT
• Sequence Length: 248

• Applicable Applications for SFRS1 recombinant protein: SDS-PAGE
• Antigen Species: Human
• Tag Information: His-tag
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for SFRS1 recombinant protein

Serine/arginine-rich splicing factor 1, also known as SFRS1, is a member of the arginine/serine-rich splicing factor protein family, and functions in both constitutive and alternative pre-mRNA splicing. This protein binds to pre-mRNA transcripts and components of the spliceosome, and can either activate or repress splicing depending on the location of the pre-mRNA binding site. The protein's ability to activate splicing is regulated by phosphorylation and interactions with other splicing factor associated proteins. Recombinant human SFRS1 protein, fused to His-tag at N-terminus, was expressed in E Coli.

Product Categories/Family for SFRS1 recombinant protein

Epigenomics

References

Zuo P., et al. (1993) EMBO J. 12:4727-4737; Kohtz J.D., et al. (1994) Nature. 368:119-124;

NCBI and Uniprot Product Information

• NCBI GI #: 5902076
• NCBI GeneID: 6426
• NCBI Accession #: NP_008855
• NCBI GenBank Nucleotide #: NM_006924.4
• UniProt Accession #: Q07955; Q13809; B2R6Z7; D3DTZ3
• Molecular Weight: 29.9 kDa (268aa)
• NCBI Official Full Name: serine/arginine-rich splicing factor 1 isoform 1
• NCBI Official Synonym Full Names: serine and arginine rich splicing factor 1
• NCBI Official Symbol: SRSF1
• NCBI Official Synonym Symbols: ASF; SF2; SFRS1; SF2p33; SRp30a
• NCBI Protein Information: serine/arginine-rich splicing factor 1
• UniProt Protein Name: Serine/arginine-rich splicing factor 1
• UniProt Gene Name: SRSF1
• UniProt Synonym Gene Names: ASF; SF2; SF2P33; SFRS1; ASF-1

NCBI Description

This gene encodes a member of the arginine/serine-rich splicing factor protein family. The encoded protein can either activate or repress splicing, depending on its phosphorylation state and its interaction partners. Multiple transcript variants have been found for this gene. There is a pseudogene of this gene on chromosome 13. [provided by RefSeq, Jun 2014]

Uniprot Description

Plays a role in preventing exon skipping, ensuring the accuracy of splicing and regulating alternative splicing. Interacts with other spliceosomal components, via the RS domains, to form a bridge between the 5'- and 3'-splice site binding components, U1 snRNP and U2AF. Can stimulate binding of U1 snRNP to a 5'-splice site-containing pre-mRNA. Binds to purine-rich RNA sequences, either the octamer, 5'-RGAAGAAC-3' (r=A or G) or the decamers, AGGACAGAGC/AGGACGAAGC. Binds preferentially to the 5'-CGAGGCG-3' motif in vitro. Three copies of the octamer constitute a powerful splicing enhancer in vitro, the ASF/SF2 splicing enhancer (ASE) which can specifically activate ASE-dependent splicing. Isoform ASF-2 and isoform ASF-3 act as splicing repressors. May function as export adapter involved in mRNA nuclear export through the TAP/NXF1 pathway.