PRICKLE1 blocking peptide

PRICKLE1 Peptide

Cat. Num. AAA152296

• Gene Names: PRICKLE1; RILP; EPM1B
• Applications: Blocking (BL)
• Synonyms: PRICKLE1; PRICKLE1 Peptide; RILP; EPM1B; Prickle-like protein 1; prickle homolog 1; PRICKLE1 blocking peptide

• Form/Format: Liquid
• Concentration: 200 ug/mL (varies by lot)
• Sequence Length: 831

• Applicable Applications for PRICKLE1 blocking peptide: Blocking (BL)
• Application Notes: PRICKLE1 peptide is used for blocking the activity of PRICKLE1 antibody.
• Species: Human
• Buffer: PBS pH 7.2 (10 mM NaH2PO4, 10 mM Na2HPO4, 130 mM NaCl) containing 0.1% bovine serum albumin and 0.02% sodium azide
• Location: 19 amino acids near the carboxy terminus of human PRICKLE1.
• Preparation and Storage: Store PRICKLE1 peptide at -20 degree C, stable for one year.

NCBI and Uniprot Product Information

• NCBI GI #: 222136684
• NCBI GeneID: 144165
• NCBI Accession #: NP_694571
• NCBI GenBank Nucleotide #: NM_001144883.1
• UniProt Accession #: Q96MT3; Q14C83; Q71QF8; Q96N00
• Molecular Weight: 94,300 Da
• NCBI Official Full Name: prickle-like protein 1
• NCBI Official Synonym Full Names: prickle homolog 1 (Drosophila)
• NCBI Official Symbol: PRICKLE1
• NCBI Official Synonym Symbols: RILP; EPM1B
• NCBI Protein Information: prickle-like protein 1; prickle-like 1; REST/NRSF-interacting LIM domain protein 1; REST (RE-1 silencing transcription factor)/NRSF (neuron-restrictive silencer factor)-interacting LIM domain protein
• UniProt Protein Name: Prickle-like protein 1
• Protein Family: Prickle-like protein
• UniProt Gene Name: PRICKLE1
• UniProt Synonym Gene Names: RILP
• UniProt Entry Name: PRIC1_HUMAN

NCBI Description

This gene encodes a nuclear receptor that may be a negative regulator of the Wnt/beta-catenin signaling pathway. The encoded protein localizes to the nuclear membrane and has been implicated in the nuclear trafficking of the transcription repressors REST/NRSF and REST4. Mutations in this gene have been linked to progressive myoclonus epilepsy. Alternate splicing results in multiple transcript variants. A pseudogene of this gene is found on chromosome 3. [provided by RefSeq, Sep 2009]

Uniprot Description

PRICKLE1: Involved in the planar cell polarity pathway that controls convergent extension during gastrulation and neural tube closure. Convergent extension is a complex morphogenetic process during which cells elongate, move mediolaterally, and intercalate between neighboring cells, leading to convergence toward the mediolateral axis and extension along the anteroposterior axis. Necessary for nuclear localization of REST. May serve as nuclear receptor. Defects in PRICKLE1 are the cause of progressive myoclonic epilepsy type 1B (EPM1B). EPM1B is an autosomal recessive disorder characterized by myoclonus that progresses in severity over time, tonic-clonic seizures and ataxia. Defects in PRICKLE1 may be a cause of susceptibility to neural tube defects (NTD). Congenital malformations of the central nervous system and adjacent structures related to defective neural tube closure during the first trimester of pregnancy. Failure of neural tube closure can occur at any level of the embryonic axis. Common NTD forms include anencephaly, myelomeningocele and spina bifida, which result from the failure of fusion in the cranial and spinal region of the neural tube. NTDs have a multifactorial etiology encompassing both genetic and environmental components. Belongs to the prickle / espinas / testin family.

Protein type: Unknown function

Chromosomal Location of Human Ortholog: 12q12

Cellular Component: nuclear membrane; nucleus; cytosol

Molecular Function: protein binding; zinc ion binding

Biological Process: positive regulation of protein ubiquitination; protein import into nucleus; positive regulation of proteasomal ubiquitin-dependent protein catabolic process; neural tube closure; negative regulation of transcription, DNA-dependent

Disease: Epilepsy, Progressive Myoclonic 1b