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WB (Western Blot)
(Figure 4. Western BlotSample: Active recombinant CEA, HumanAntibody: Rabbit Anti-Human CEA Ab)
Carcinoembryonic Antigen Active Protein | CEA active protein
Active Carcinoembryonic Antigen (CEA)
Gene Names
CEACAM5; CEA; CD66e
Applications
Activity Assay, Cell Culture
Purity
>98%
Synonyms
Carcinoembryonic Antigen; N/A; Active Carcinoembryonic Antigen (CEA); CD66E; CD66; CEACAM5; Carcinoembryonic Antigen-related Cell Adhesion Molecule 5; CEA active protein
Host
E. coli
Purity/Purification
>98%
Form/Format
20mM Tris, 150mM NaCl, pH8.0, containing 0.01% skl and 5% trehalose.
Concentration
Original Concentration: 50ug/mL (varies by lot)
Applicable Applications for CEA active protein
Activity Assay, Cell Culture
Organism Species
Homo sapiens (Human)
Source
Prokaryotic expression.
Residues
Ala566~Gly698
Tags
N-terminal His-tag
Endotoxin Level
<1.0EU per 1ug (determined by the LAL method).
Predicted isoelectric point
5.9
Phenomenon explanation
The possible reasons that the actual band size differs from the predicted are as follows:
1. Splice variants: Alternative splicing may create different sized proteins from the same gene.
2. Relative charge: The composition of amino acids may affects the charge of the protein.
3. Post-translational modification: Phosphorylation, glycosylation, methylation etc.
4. Post-translation cleavage: Many proteins are synthesized as pro-proteins, and then cleaved to give the active form.
5. Polymerization of the target protein: Dimerization, multimerization etc.
1. Splice variants: Alternative splicing may create different sized proteins from the same gene.
2. Relative charge: The composition of amino acids may affects the charge of the protein.
3. Post-translational modification: Phosphorylation, glycosylation, methylation etc.
4. Post-translation cleavage: Many proteins are synthesized as pro-proteins, and then cleaved to give the active form.
5. Polymerization of the target protein: Dimerization, multimerization etc.
Usage
Reconstitute in ddH2O to a concentration of 0-0.05mg/mL. Do not vortex.
Preparation and Storage
Storage:
Avoid repeated freeze/thaw cycles.
Store at 2-8°C for one month.
Aliquot and store at -80°C for 12 months.
Stability Test:
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Avoid repeated freeze/thaw cycles.
Store at 2-8°C for one month.
Aliquot and store at -80°C for 12 months.
Stability Test:
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
WB (Western Blot)
(Figure 4. Western BlotSample: Active recombinant CEA, HumanAntibody: Rabbit Anti-Human CEA Ab)
WB (Western Blot)
(Figure 4. Western BlotSample: Active recombinant CEA, HumanAntibody: Rabbit Anti-Human CEA Ab)
SDS_PAGE
(Figure 3. SDS-PAGESample: Active recombinant CEA, Human)
SDS_PAGE
(Figure 3. SDS-PAGESample: Active recombinant CEA, Human)
Gene Sequencing
(Figure.2 Gene Sequencing (Extract))
Gene Sequencing
(Figure.2 Gene Sequencing (Extract))
Bioactivity
(Carcinoembryonic antigen (CEA) which are characterized as members of the CD66 cluster of differentiation have highly related glycoproteins involved in cell adhesion. It also can be used as a tumor marker in clinical tests. CEA are glycosyl phosphatidyl inositol (GPI) cell-surface-anchored glycoproteins whose specialized sialofucosylated glycoforms serve as functional colon carcinoma L-selectin and E-selectin ligands. Besides, Galectin 4 (GAL4) has been identified as an interactor of CEA, thus a binding ELISA assay was conducted to detect the interaction of recombinant human CEA and recombinant human GLA4. Briefly, CEA were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to GLA4-coated microtiter wells and incubated for 2h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-CEA pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of CEA and GLA4 was shown in Figure 1, and this effect was in a dose dependent manner.)
Bioactivity
(Carcinoembryonic antigen (CEA) which are characterized as members of the CD66 cluster of differentiation have highly related glycoproteins involved in cell adhesion. It also can be used as a tumor marker in clinical tests. CEA are glycosyl phosphatidyl inositol (GPI) cell-surface-anchored glycoproteins whose specialized sialofucosylated glycoforms serve as functional colon carcinoma L-selectin and E-selectin ligands. Besides, Galectin 4 (GAL4) has been identified as an interactor of CEA, thus a binding ELISA assay was conducted to detect the interaction of recombinant human CEA and recombinant human GLA4. Briefly, CEA were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to GLA4-coated microtiter wells and incubated for 2h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-CEA pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of CEA and GLA4 was shown in Figure 1, and this effect was in a dose dependent manner.)
Sequence
Sequence
Product Categories/Family for CEA active protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
Predicted Molecular Mass: 15.0kDa
Accurate Molecular Mass: 16kDa as determined by SDS-PAGE reducing conditions.
Accurate Molecular Mass: 16kDa as determined by SDS-PAGE reducing conditions.
NCBI Official Full Name
Carcinoembryonic antigen-related cell adhesion molecule 5
NCBI Official Synonym Full Names
CEA cell adhesion molecule 5
NCBI Official Symbol
CEACAM5
NCBI Official Synonym Symbols
CEA; CD66e
NCBI Protein Information
carcinoembryonic antigen-related cell adhesion molecule 5
UniProt Protein Name
Carcinoembryonic antigen-related cell adhesion molecule 5
UniProt Gene Name
CEACAM5
UniProt Synonym Gene Names
CEA; CEA
UniProt Entry Name
CEAM5_HUMAN
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Product Notes
The CEA ceacam5 (Catalog #AAA150079) is an Active Protein produced from E. coli and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Carcinoembryonic Antigen can be used in a range of immunoassay formats including, but not limited to, Activity Assay, Cell Culture. Researchers should empirically determine the suitability of the CEA ceacam5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Carcinoembryonic Antigen, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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