Rabbit SDF-1/CXCL12 ELISA Kit | SDF-1/CXCL12 elisa kit
Rabbit SDF-1/CXCL12 (Stromal Cell Derived Factor 1) ELISA Kit
Gene Names
CXCL12; SDF1
Reactivity
Rabbit
Synonyms
SDF-1/CXCL12; Rabbit SDF-1/CXCL12 (Stromal Cell Derived Factor 1) ELISA Kit; SDF-1/CXCL12 elisa kit
Reactivity
Rabbit
Specificity
This assay has high sensitivity and excellent specificity for detection of Rabbit SDF-1/CXCL12. No significant cross-reactivity or interference between Rabbit SDF-1/CXCL12 and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between Rabbit SDF-1/CXCL12 and all the analogues, therefore, cross reaction may still exist.
Sequence Length
89
Samples
Serum, Plasma, Biological Fluids
Assay Type
Sandwich
Detection Range
0.156-10ng/mL
Sensitivity
Min: 0.094ng/mL; Max: 10ng/mL
Preparation and Storage
Store at 4 degree C.
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for SDF-1/CXCL12 elisa kit
Description: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of SDF-1/CXCL12 in Rabbit serum, plasma and other biological fluids
Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rabbit SDF-1/CXCL12. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Rabbit SDF-1/CXCL12 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rabbit SDF-1/CXCL12, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Rabbit SDF-1/CXCL12. You can calculate the concentration of Rabbit SDF-1/CXCL12 in the samples by comparing the OD of the samples to the standard curve.
Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rabbit SDF-1/CXCL12. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Rabbit SDF-1/CXCL12 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rabbit SDF-1/CXCL12, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Rabbit SDF-1/CXCL12. You can calculate the concentration of Rabbit SDF-1/CXCL12 in the samples by comparing the OD of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
stromal cell-derived factor 1
NCBI Official Synonym Full Names
chemokine (C-X-C motif) ligand 12
NCBI Official Symbol
CXCL12
NCBI Official Synonym Symbols
SDF1
NCBI Protein Information
stromal cell-derived factor 1; chemokine (C-X-C motif) ligand 12 (stromal cell-derived factor 1); chemokine ligand 12b; stromal cell derived factor 1; stromal cell-derived factor-1 beta
