MARCH5 blocking peptide

MARCH5 Antibody (N-term) Blocking peptide

• Gene Names: MARCH5; MITOL; RNF153; MARCH-V
• Synonyms: MARCH5; MARCH5 Antibody (N-term) Blocking peptide; E3 ubiquitin-protein ligase MARCH5; 632-; Membrane-associated RING finger protein 5; Membrane-associated RING-CH protein V; MARCH-V; Mitochondrial ubiquitin ligase; MITOL; RING finger protein 153; RNF153; MARCH5 blocking peptide

• Form/Format: Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.
• Sequence Length: 278

• Cellular Location: Mitochondrion outer membrane; Multi-pass membrane protein. Endoplasmic reticulum membrane. Note: Authors show that the protein can be detected in endoplasmic reticulum (PubMed:14722266). Authors (PubMed:16874301) show its presence only in mitochondria (PubMed:16874301)
• Tissue Location: Expressed in brain, heart, liver, lung, spleen, stomach, testis, skeletal and muscle
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Related Product Information for MARCH5 blocking peptide

Mitochondrial E3 ubiquitin-protein ligase that plays a crucial role in the control of mitochondrial morphology by acting as a positive regulator of mitochondrial fission. May play a role in the prevention of cell senescence acting as a regulator of mitochondrial quality control. Promotes ubiquitination of FIS1, DNM1L and MFN1.

NCBI and Uniprot Product Information

• NCBI GI #: 8923415
• NCBI GeneID: 54708
• NCBI Accession #: NP_060294
• NCBI GenBank Nucleotide #: NM_017824.4
• UniProt Accession #: Q9NX47
• Molecular Weight: 31,232 Da
• NCBI Official Full Name: E3 ubiquitin-protein ligase MARCH5
• NCBI Official Synonym Full Names: membrane associated ring-CH-type finger 5
• NCBI Official Symbol: MARCH5
• NCBI Official Synonym Symbols: MITOL; RNF153; MARCH-V
• NCBI Protein Information: E3 ubiquitin-protein ligase MARCH5
• UniProt Protein Name: E3 ubiquitin-protein ligase MARCH5
• UniProt Gene Name: MARCH5
• UniProt Synonym Gene Names: RNF153; MARCH-V; MITOL
• UniProt Entry Name: MARH5_HUMAN

NCBI Description

MARCH5 is a ubiquitin ligase of the mitochondrial outer membrane that plays a role in the control of mitochondrial morphology by regulating mitofusin-2 (MFN2; MIM 608507) and DRP1 (DNM1L; MIM 603850) (Nakamura et al., 2006 [PubMed 16936636]).[supplied by OMIM, Mar 2008]

Uniprot Description

MARCH5: Mitochondrial E3 ubiquitin-protein ligase that plays a crucial role in the control of mitochondrial morphology by acting as a positive regulator of mitochondrial fission. May play a role in the prevention of cell senescence acting as a regulator of mitochondrial quality control. Promotes ubiquitination of FIS1, DNM1L and MFN1.

Protein type: Ligase; EC 6.3.2.19; Ubiquitin ligase; Ubiquitin conjugating system; EC 6.3.2.-; Membrane protein, integral; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 10q23.32-q23.33

Cellular Component: endoplasmic reticulum; endoplasmic reticulum membrane; membrane; mitochondrial outer membrane

Molecular Function: GTPase binding; protein binding; ubiquitin-protein ligase activity

Biological Process: protein autoubiquitination; protein polyubiquitination