Human Tar DNA Binding Protein 43kDa (TDP43) ELISA Kit | TDP43 elisa kit

Human Tar DNA Binding Protein 43kDa (TDP43) ELISA Kit

Cat. Num. AAA2703945

• Reactivity: Human
• Synonyms: Tar DNA Binding Protein 43kDa (TDP43); Human Tar DNA Binding Protein 43kDa (TDP43) ELISA Kit; TARDBP; ALS10; TDP43 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of TDP43. No significant cross-reactivity or interference between TDP43 and analogues was observed.
• Sequence Length: 298

• Samples: Serum, plasma, cerebrospinal fluid and other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 62.5-4,000pg/mL
• Sensitivity: < 25.1pg/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level TDP43 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level TDP43 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for TDP43 elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of TDP43 in human serum, plasma, cerebrospinal fluid and other biological fluids.

Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to TDP43. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to TDP43. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain TDP43, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of TDP43 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 130750552
• Molecular Weight: 31,808 Da
• NCBI Official Full Name: TDP43
• UniProt Protein Name: TAR DNA-binding protein 43
• UniProt Gene Name: TARDBP
• UniProt Synonym Gene Names: TDP43; TDP-43

Uniprot Description

DNA and RNA-binding protein which regulates transcription and splicing. Involved in the regulation of CFTR splicing. It promotes CFTR exon 9 skipping by binding to the UG repeated motifs in the polymorphic region near the 3'-splice site of this exon. The resulting aberrant splicing is associated with pathological features typical of cystic fibrosis. May also be involved in microRNA biogenesis, apoptosis and cell division. Can repress HIV-1 transcription by binding to the HIV-1 long terminal repeat. Stabilizes the low molecular weight neurofilament (NFL) mRNA through a direct interaction with the 3' UTR.