Mouse Caspase 1 (CASP1) ELISA Kit | CASP1 elisa kit

Mouse Caspase 1 (CASP1) Mini Samples ELISA Kit

Cat. Num. AAA2708360

• Gene Names: Casp1; ICE; Il1bc
• Reactivity: Mouse
• Applications: ELISA
• Synonyms: Caspase 1 (CASP1); Mouse Caspase 1 (CASP1) Mini Samples ELISA Kit; Caspase 1; ICE; IL1BC; IL1-BC; P45; Interleukin 1 Beta Converting Enzyme; Apoptosis-Related Cysteine Peptidase; Interleukin-1 beta convertase; CASP1 elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of Mini Samples Caspase 1 (CASP1).; No significant cross-reactivity or interference between Mini Samples Caspase 1 (CASP1) and analogues was observed.
• Sequence Length: 402

• Applicable Applications for CASP1 elisa kit: ELISA (EIA)
• Assay Type: Double-antibody Sandwich
• Samples: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Detection Range: 31.2-2,000pg/mL
• Sensitivity: The minimum detectable dose of this kit is typically less than 12.2pg/mL
• Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Mini Samples Caspase 1 (CASP1) were tested 20 times on one plate, respectively.; Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Mini Samples Caspase 1 (CASP1) were tested on 3 different plates, 8 replicates in each plate.; CV(%) = SD/meanX100; Intra-Assay: CV<10%; Inter-Assay: CV<12%
• Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mini Samples Caspase 1 (CASP1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Mini Samples Caspase 1 (CASP1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mini Samples Caspase 1 (CASP1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Mini Samples Caspase 1 (CASP1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

NCBI and Uniprot Product Information

• NCBI GI #: 266322
• NCBI GeneID: 12362
• UniProt Accession #: P29452
• NCBI Official Full Name: Caspase-1
• NCBI Official Synonym Full Names: caspase 1
• NCBI Official Symbol: Casp1
• NCBI Official Synonym Symbols: ICE; Il1bc
• NCBI Protein Information: caspase-1
• UniProt Protein Name: Caspase-1
• UniProt Gene Name: Casp1
• UniProt Synonym Gene Names: Il1bc; CASP-1; IL-1BC; ICE; IL-1 beta-converting enzyme
• UniProt Entry Name: CASP1_MOUSE

Uniprot Description

CASP1: Thiol protease that cleaves IL-1 beta between an Asp and an Ala, releasing the mature cytokine which is involved in a variety of inflammatory processes. Important for defense against pathogens. Cleaves and activates sterol regulatory element binding proteins (SREBPs). Can also promote apoptosis. Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a 20 kDa (p20) and a 10 kDa (p10) subunit. The p20 subunit can also form a heterodimer with the epsilon isoform which then has an inhibitory effect. May be a component of the inflammasome, a protein complex which also includes PYCARD, CARD8 and NALP2 and whose function would be the activation of proinflammatory caspases. Interacts with CARD17/INCA and CARD18. Expressed in larger amounts in spleen and lung. Detected in liver, heart, small intestine, colon, thymus, prostate, skeletal muscle, peripheral blood leukocytes, kidney and testis. No expression in the brain. Specifically inhibited by the cowpox virus Crma protein. Belongs to the peptidase C14A family. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: Apoptosis; EC 3.4.22.36; Protease

Cellular Component: neuron projection; protein complex; mitochondrion; cytoplasm; extracellular region; nucleus

Molecular Function: peptidase activity; protein binding; hydrolase activity; endopeptidase activity; cysteine-type peptidase activity

Biological Process: response to drug; positive regulation of I-kappaB kinase/NF-kappaB cascade; positive regulation of apoptosis; apoptosis; membrane hyperpolarization; microglial cell activation; interleukin-1 beta production; response to lipopolysaccharide; mitochondrial depolarization; positive regulation of interleukin-1 beta secretion; proteolysis; response to organic cyclic substance; memory; response to ATP; positive regulation of interleukin-1 alpha secretion; regulation of apoptosis; response to bacterium; regulation of inflammatory response; response to hypoxia; protein processing; regulation of autophagy; positive regulation of circadian sleep/wake cycle, non-REM sleep; myoblast fusion; positive regulation of cytokine secretion