Itk blocking peptide

Mouse Itk Blocking Peptide (N-term)

• Gene Names: Itk; Emt; Tsk; Tcsk
• Synonyms: Itk; Mouse Itk Blocking Peptide (N-term); Tyrosine-protein kinase ITK/TSK; IL-2-inducible T-cell kinase; Kinase EMT; Kinase TLK; T-cell-specific kinase; Emt; Tlk; Tsk; Itk blocking peptide

• Specificity: The synthetic peptide sequence is selected from aa 156-170 of HUMAN Itk
• Form/Format: Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.
• Sequence Length: 625

• Cellular Location: Cytoplasm. Note: Localizes in the vicinity of cell surface receptors in the plasma membrane after receptor stimulation.
• Tissue Location: Is detected in the thymus, lymph node and very faintly in the spleen, but is not detected in the liver, lung, kidney, heart, brain, intestine or testis. Expressed in T- lymphocytes and mast cells. It may also be expressed in natural killer cells
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Related Product Information for Itk blocking peptide

Tyrosine kinase that plays an essential role in regulation of the adaptive immune response. Regulates the development, function and differentiation of conventional T-cells and nonconventional NKT-cells. When antigen presenting cells (APC) activate T-cell receptor (TCR), a series of phosphorylation lead to the recruitment of ITK to the cell membrane, in the vicinity of the stimulated TCR receptor, where it is phosphorylated by LCK. Phosphorylation leads to ITK autophosphorylation and full activation. Once activated, phosphorylates PLCG1, leading to the activation of this lipase and subsequent cleavage of its substrates. In turn, the endoplasmic reticulum releases calcium in the cytoplasm and the nuclear activator of activated T-cells (NFAT) translocates into the nucleus to perform its transcriptional duty. Phosphorylates 2 essential adapter proteins: the linker for activation of T-cells/LAT protein and LCP2. Then, a large number of signaling molecules such as VAV1 are recruited and ultimately lead to lymphokine production, T-cell proliferation and differentiation.

NCBI and Uniprot Product Information

• NCBI GI #: 729903
• NCBI GeneID: 16428
• UniProt Accession #: Q03526; Q08881
• Molecular Weight: 72,292 Da
• NCBI Official Full Name: Tyrosine-protein kinase ITK/TSK
• NCBI Official Synonym Full Names: IL2 inducible T cell kinase
• NCBI Official Symbol: Itk
• NCBI Official Synonym Symbols: Emt; Tsk; Tcsk
• NCBI Protein Information: tyrosine-protein kinase ITK/TSK
• UniProt Protein Name: Tyrosine-protein kinase ITK/TSK
• Protein Family: Tyrosine-protein kinase
• UniProt Gene Name: Itk
• UniProt Synonym Gene Names: Emt; Tlk; Tsk
• UniProt Entry Name: ITK_MOUSE

Uniprot Description

ITK: an intracellular tyrosine kinase of the Tec family expressed in T-cells. The protein contains both SH2 and SH3 domains and plays a role in T-cell proliferation and differentiation. Itk-deficient murine T cells display defects in TCR/CD3-induced actin polymerization.

Protein type: Protein kinase, tyrosine (non-receptor); EC 2.7.10.2; Protein kinase, TK; Kinase, protein; TK group; Tec family

Cellular Component: extrinsic to internal side of plasma membrane; intercellular junction

Molecular Function: non-membrane spanning protein tyrosine kinase activity; protein binding

Biological Process: adaptive immune response; cytokine production; innate immune response; interferon-gamma production; interleukin-4 production; NK T cell differentiation; phospholipase C activation; regulation of cell proliferation; T cell receptor signaling pathway; transmembrane receptor protein tyrosine kinase signaling pathway