Rabbit ACTR3 Polyclonal Antibody | anti-ACTR3 antibody

ACTR3 Polyclonal Antibody

Cat. Num. AAA3005693

• Gene Names: ACTR3; ARP3
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: ACTR3; Polyclonal Antibody; ACTR3 Polyclonal Antibody; Actin-like protein 3; ARP3; Actin-related protein 3; anti-ACTR3 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: ACTR3 polyclonal antibody detects endogenous levels of ACTR3 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 418

• Applicable Applications for anti-ACTR3 antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: WB: 1:500-1:1000; IHC: 1:50-1:200
• Immunogen: A synthetic peptide corresponding to residues in Human ACTR3.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of ACTR3 polyclonal antibody at 1:500 dilutionLane1:Hela cell lysateLane2:NIH-3T3 cell lysateLane3:PC12 cell lysate)

Immunohistochemistry (IHC)

(Immunohistochemistry (IHC) analyzes of ACTR3 pAb in paraffin-embedded human tonsil carcinoma tissue at 1:50,showing cytoplasmic and perinuclear staining. Negative control (the right) Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.)

Immunohistochemistry (IHC)

(Immunohistochemistry (IHC) analyzes of ACTR3 pAb in paraffin-embedded human stomach carcinoma tissue at 1:50,showing cytoplasmic and perinuclear staining. Negative control (the right) Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.)

Immunohistochemistry (IHC)

(Immunohistochemistry (IHC) analyzes of ACTR3 pAb in paraffin-embedded human rectum carcinoma tissue at 1:50,showing cytoplasmic and perinuclear staining. Negative control (the right) Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.)

Related Product Information for anti-ACTR3 antibody

Actin polymerization is required for a variety of cell functions, including chemotaxis, cell migration, cell adhesion, and platelet activation. Cells trigger actin polymerization through either the de novo nucleation of filaments from monomeric actin, the severing of existing filaments to create uncapped barbed ends, or the uncapping existing barbed ends. The nucleation of actin is a rate-limiting and unfavorable reaction in actin polymerization and therefore requires the involvement of the Arp2/3 complex, which helps create new filaments and promotes the end-to-side cross-linking of actin filaments into the branching meshwork. The Arp2/3 complex consists of the actin-related proteins Arp2 and Arp3, and various other accessory proteins. The Arp2/3 complex promotes actin nucleation by binding the pointed end of actin filaments, or by associating with the side of an existing filament, and nucleates growth in the barbed direction. In addition, the Arp2/3 complex also mediates actin cytoskeletal outgrowths that are regulated by the Rho family of small GTPases. In response to GTP-binding Cdc42, the Arp2/3 complex binds the Cdc42 substrates, namely the WASP proteins, and initiates the formation of lamellipodia and filopodia.

NCBI and Uniprot Product Information

• NCBI GI #: 5031573
• NCBI GeneID: 10096
• NCBI Accession #: NP_005712.1
• NCBI GenBank Nucleotide #: NP_005712.1
• UniProt Accession #: P61158
• Molecular Weight: ~ 48kDa
• NCBI Official Full Name: actin-related protein 3 isoform 1
• NCBI Official Synonym Full Names: actin related protein 3
• NCBI Official Symbol: ACTR3
• NCBI Official Synonym Symbols: ARP3
• NCBI Protein Information: actin-related protein 3
• UniProt Protein Name: Actin-related protein 3
• UniProt Gene Name: ACTR3
• UniProt Synonym Gene Names: ARP3
• UniProt Entry Name: ARP3_HUMAN

NCBI Description

The specific function of this gene has not yet been determined; however, the protein it encodes is known to be a major constituent of the ARP2/3 complex. This complex is located at the cell surface and is essential to cell shape and motility through lamellipodial actin assembly and protrusion. Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Mar 2013]

Uniprot Description

Arp3: Functions as ATP-binding component of the Arp2/3 complex which is involved in regulation of actin polymerization and together with an activating nucleation-promoting factor (NPF) mediates the formation of branched actin networks. Seems to contact the pointed end of the daughter actin filament. Plays a role in ciliogenesis. Component of the Arp2/3 complex composed of ARP2, ARP3, ARPC1B/p41-ARC, ARPC2/p34-ARC, ARPC3/p21-ARC, ARPC4/p20-ARC and ARPC5/p16-ARC. Interacts with WHDC1. Belongs to the actin family. ARP3 subfamily.

Protein type: Cytoskeletal; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 2q14.1

Cellular Component: Golgi membrane; Arp2/3 protein complex; focal adhesion; hemidesmosome; membrane; lamellipodium; excitatory synapse; actin filament; podosome; intercellular junction; cytosol; actin cytoskeleton

Molecular Function: actin filament binding; protein binding; structural constituent of cytoskeleton; ATP binding

Biological Process: positive regulation of filopodium formation; meiotic chromosome movement towards spindle pole; axon guidance; regulation of myosin II filament assembly or disassembly; establishment and/or maintenance of cell polarity; positive regulation of dendrite morphogenesis; cytokinesis after meiosis; response to carbohydrate stimulus; spindle localization; response to antibiotic; asymmetric cell division; ephrin receptor signaling pathway; innate immune response; cell motility