HOOK1 blocking peptide

HOOK1 Antibody (Center) Blocking Peptide

• Gene Names: HOOK1; HK1
• Synonyms: HOOK1; HOOK1 Antibody (Center) Blocking Peptide; Protein Hook homolog 1; h-hook1; hHK1; HOOK1 blocking peptide

• Specificity: The synthetic peptide sequence used to generate the antibody was selected from the Center region of HOOK1. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.
• Form/Format: Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.
• Sequence Length: 728

• Cellular Location: Cytoplasm, cytoskeleton. Cytoplasm. Note: Localizes to the spermatid manchette during spermiogenesis but is not present in mature spermatozoa (By similarity). Localizes to punctate cytoplasmic foci which do not appear to overlap with early or late endosomes, the endoplasmic reticulum, the Golgi complex, multivesicular bodies (MVBs), lysosomes, or mitochondria. Often found in close association with microtubules.
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Related Product Information for HOOK1 blocking peptide

Required for spermatid differentiation. Probably involved in the positioning of the microtubules of the manchette and the flagellum in relation to the membrane skeleton (By similarity). Component of the FTS/Hook/FHIP complex (FHF complex). The FHF complex may function to promote vesicle trafficking and/or fusion via the homotypic vesicular protein sorting complex (the HOPS complex).

NCBI and Uniprot Product Information

• NCBI GI #: 41688595
• NCBI GeneID: 51361
• UniProt Accession #: Q9UJC3; O60561; Q5TG44; A8K8E9; A8MU44; B4DX15
• Molecular Weight: 80,021 Da
• NCBI Official Full Name: Protein Hook homolog 1
• NCBI Official Synonym Full Names: hook microtubule tethering protein 1
• NCBI Official Symbol: HOOK1
• NCBI Official Synonym Symbols: HK1
• NCBI Protein Information: protein Hook homolog 1
• UniProt Protein Name: Protein Hook homolog 1
• Protein Family: Protein Hook
• UniProt Gene Name: HOOK1
• UniProt Synonym Gene Names: h-hook1; hHK1
• UniProt Entry Name: HOOK1_HUMAN

NCBI Description

This gene encodes a member of the hook family of coiled-coil proteins, which bind to microtubules and organelles through their N- and C-terminal domains, respectively. The encoded protein localizes to discrete punctuate subcellular structures, and interacts with several members of the Rab GTPase family involved in endocytosis. It is thought to link endocytic membrane trafficking to the microtubule cytoskeleton. Several alternatively spliced transcript variants have been identified, but the full-length nature of some of these variants has not been determined. [provided by RefSeq, Jul 2008]

Uniprot Description

HOOK1: Required for spermatid differentiation. Probably involved in the positioning of the microtubules of the manchette and the flagellum in relation to the membrane skeleton. Component of the FTS/Hook/FHIP complex (FHF complex). The FHF complex may function to promote vesicle trafficking and/or fusion via the homotypic vesicular protein sorting complex (the HOPS complex). Belongs to the hook family.

Protein type: Cytoskeletal

Chromosomal Location of Human Ortholog: 1p32.1

Molecular Function: identical protein binding; protein binding

Biological Process: early endosome to late endosome transport; endosome organization and biogenesis; endosome to lysosome transport; lysosome organization and biogenesis