Rabbit HOOK1 Polyclonal Antibody | anti-HOOK1 antibody

HOOK1 Antibody

Cat. Num. AAA9611388

• Gene Names: HOOK1; HK1
• Reactivity: Human, Mouse; Predicted: Bovine, Horse, Sheep, Rabbit, Dog
• Applications: Western Blot
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Synonyms: HOOK1; Polyclonal Antibody; HOOK1 Antibody; A930033L17Rik; Abnormal spermatozoon head shape; azh; h-hook1; hHK1; HK1; HOOK 1; Hook homolog 1 (Drosophila); Hook1; HOOK1_HUMAN; MGC10642; OTTHUMP00000010548; OTTMUSP00000008480; Protein Hook homolog 1; RP23-80B16.4; anti-HOOK1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse; Predicted: Bovine, Horse, Sheep, Rabbit, Dog
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: HOOK1 Antibody detects endogenous levels of HOOK1.
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Form/Format: Liquid; Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 728

• Applicable Applications for anti-HOOK1 antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:2000
• Immunogen: A synthesized peptide derived from human HOOK1.
• Fragment: Fab Fragment
• Conjugate: Unconjugated
• Subcellular Location: Cytoskeleton
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Western Blot (WB)

(Western blot analysis of extracts from HEK-293 cells, using HOOK1 Antibody. The lane on the left was treated with blocking peptide.)

Related Product Information for anti-HOOK1 antibody

Description: Required for spermatid differentiation. Probably involved in the positioning of the microtubules of the manchette and the flagellum in relation to the membrane skeleton (By similarity). Component of the FTS/Hook/FHIP complex (FHF complex). The FHF complex may function to promote vesicle trafficking and/or fusion via the homotypic vesicular protein sorting complex (the HOPS complex) (PubMed:18799622).

Subunit Structure: Self-associates (PubMed:18799622). Component of the FTS/Hook/FHIP complex (FHF complex), composed of AKTIP/FTS, FAM160A2, and one or more members of the Hook family of proteins HOOK1, HOOK2, and HOOK3 (PubMed:18799622). May interact directly with AKTIP/FTS, HOOK2 and HOOK3 (PubMed:18799622). Associates with several subunits of the homotypic vesicular sorting complex (the HOPS complex) including VPS16, VPS18, VPS39 and VPS41; these interactions may be indirect (PubMed:18799622). Interacts with CCDC181 (By similarity). Interacts (via coiled-coil region) with RIMBP3 (via C-terminus) (By similarity). Interacts with LRGUK (via guanylate kinase-like domain) (By similarity). Interacts with microtubules (PubMed:11238449).

Similarity: Belongs to the hook family.

NCBI and Uniprot Product Information

• NCBI GI #: 7705415
• NCBI GeneID: 51361
• NCBI Accession #: NP_056972.1
• NCBI GenBank Nucleotide #: NP_056972.1
• UniProt Accession #: Q9UJC3
• Molecular Weight: Observed: 85 kDa; Predicted: 85 kDa
• NCBI Official Full Name: protein Hook homolog 1
• NCBI Official Synonym Full Names: hook microtubule tethering protein 1
• NCBI Official Symbol: HOOK1
• NCBI Official Synonym Symbols: HK1
• NCBI Protein Information: protein Hook homolog 1
• UniProt Protein Name: Protein Hook homolog 1
• Protein Family: Protein Hook
• UniProt Gene Name: HOOK1
• UniProt Synonym Gene Names: h-hook1; hHK1
• UniProt Entry Name: HOOK1_HUMAN

NCBI Description

This gene encodes a member of the hook family of coiled-coil proteins, which bind to microtubules and organelles through their N- and C-terminal domains, respectively. The encoded protein localizes to discrete punctuate subcellular structures, and interacts with several members of the Rab GTPase family involved in endocytosis. It is thought to link endocytic membrane trafficking to the microtubule cytoskeleton. Several alternatively spliced transcript variants have been identified, but the full-length nature of some of these variants has not been determined. [provided by RefSeq, Jul 2008]