CLP1 blocking peptide

CLP1 Antibody (C-term) Blocking Peptide

• Gene Names: CLP1; HEAB; hClp1
• Synonyms: CLP1; CLP1 Antibody (C-term) Blocking Peptide; Polyribonucleotide 5'-hydroxyl-kinase Clp1; 27178; Polyadenylation factor Clp1; Polynucleotide kinase Clp1; Pre-mRNA cleavage complex II protein Clp1; HEAB; CLP1 blocking peptide

• Form/Format: Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.
• Sequence Length: 425

• Cellular Location: Nucleus
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Related Product Information for CLP1 blocking peptide

Polynucleotide kinase that can phosphorylate the 5'- hydroxyl groups of double-stranded RNA (dsRNA), single-stranded RNA (ssRNA), double-stranded DNA (dsDNA) and double-stranded DNA:RNA hybrids. dsRNA is phosphorylated more efficiently than dsDNA, and the RNA component of a DNA:RNA hybrid is phosphorylated more efficiently than the DNA component. Plays a key role in both tRNA splicing and mRNA 3'-end formation. Component of the tRNA splicing endonuclease complex: phosphorylates the 5'-terminus of the tRNA 3'-exon during tRNA splicing; this phosphorylation event is a prerequisite for the subsequent ligation of the two exon halves and the production of a mature tRNA (PubMed:24766809, PubMed:24766810). Its role in tRNA splicing and maturation is required for cerebellar development (PubMed:24766809, PubMed:24766810). Component of the pre-mRNA cleavage complex II (CF-II), which seems to be required for mRNA 3'-end formation. Also phosphorylates the 5'-terminus of exogenously introduced short interfering RNAs (siRNAs), which is a necessary prerequisite for their incorporation into the RNA-induced silencing complex (RISC). However, endogenous siRNAs and microRNAs (miRNAs) that are produced by the cleavage of dsRNA precursors by DICER1 already contain a 5'-phosphate group, so this protein may be dispensible for normal RNA-mediated gene silencing.

NCBI and Uniprot Product Information

• NCBI GI #: 13431366
• NCBI GeneID: 10978
• UniProt Accession #: Q92989; B2R7J6; B4DTI8
• Molecular Weight: 40,696 Da
• NCBI Official Full Name: Polyribonucleotide 5'-hydroxyl-kinase Clp1
• NCBI Official Synonym Full Names: cleavage and polyadenylation factor I subunit 1
• NCBI Official Symbol: CLP1
• NCBI Official Synonym Symbols: HEAB; hClp1
• NCBI Protein Information: polyribonucleotide 5'-hydroxyl-kinase Clp1
• UniProt Protein Name: Polyribonucleotide 5'-hydroxyl-kinase Clp1
• Protein Family: Tyrosine-protein phosphatase
• UniProt Gene Name: CLP1
• UniProt Entry Name: CLP1_HUMAN

NCBI Description

This gene encodes a member of the Clp1 family. The encoded protein is a multifunctional kinase which is a component of the tRNA splicing endonuclease complex and a component of the pre-mRNA cleavage complex II. This protein is implicated in tRNA, mRNA, and siRNA maturation. Mutations in this gene are associated with pontocerebellar hypoplasia type 10 (PCH10). Alternatively splice transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2014]

Uniprot Description

HEAB: Polynucleotide kinase that can phosphorylate the 5'- hydroxyl groups of double-stranded RNA (dsRNA), single-stranded RNA (ssRNA), double stranded DNA (dsDNA) and double-stranded DNA:RNA hybrids. dsRNA is phosphorylated more efficiently than dsDNA, and the RNA component of a DNA:RNA hybrid is phosphorylated more efficiently than the DNA component. Appears to have roles in both tRNA splicing and mRNA 3'-end formation. Component of the tRNA splicing endonuclease complex. Phosphorylates the 5'-terminus of the tRNA 3'-exon during tRNA splicing; this phosphorylation event is a prerequisite for the subsequent ligation of the two exon halves and the production of a mature tRNA. Component of the pre-mRNA cleavage complex II (CF-II), which seems to be required for mRNA 3'-end formation. Also phosphorylates the 5'-terminus of exogenously introduced short interfering RNAs (siRNAs), which is a necessary prerequisite for their incorporation into the RNA- induced silencing complex (RISC). However, endogenous siRNAs and microRNAs (miRNAs) that are produced by the cleavage of dsRNA precursors by DICER1 already contain a 5'-phosphate group, so this protein may be dispensible for normal RNA-mediated gene silencing. Belongs to the Clp1 family. Clp1 subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: RNA splicing; RNA-binding; EC 2.7.1.78

Chromosomal Location of Human Ortholog: 11q12

Cellular Component: cytoplasm; nucleoplasm; nucleus; tRNA-intron endonuclease complex

Molecular Function: ATP binding; ATP-dependent polyribonucleotide 5'-hydroxyl-kinase activity; polydeoxyribonucleotide kinase activity

Biological Process: cerebellar cortex development; mRNA 3'-end processing; mRNA cleavage; mRNA polyadenylation; nuclear mRNA splicing, via spliceosome; RNA interference, siRNA loading onto RISC; RNA interference, targeting of mRNA for destruction; termination of RNA polymerase II transcription; tRNA splicing

Disease: Pontocerebellar Hypoplasia, Type 10