Rabbit anti-Human HEAB Polyclonal Antibody | anti-HEAB antibody

HEAB antibody

Cat. Num. AAA9405354

• Gene Names: CLP1; HEAB; hClp1
• Reactivity: Human
• Applications: Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: Purified by antigen-affinity chromatography.
• Synonyms: HEAB; Polyclonal Antibody; HEAB antibody; anti-HEAB antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Purity/Purification: Purified by antigen-affinity chromatography.
• Form/Format: Supplied in 0.1M Tris-buffered saline with 20% Glycerol (pH7.0). 0.01% Thimerosal was added as a preservative.
• Sequence Length: 425

• Applicable Applications for anti-HEAB antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: Immunohistochemistry: 1:100-1:250; Immunofluorescence: 1:100-1:200
• Immunogen Type: Recombinant protein
• Immunogen Description: Recombinant protein fragment contain a sequence corresponding to a region within amino acids 142 and 341 of HEAB
• Target Name: HEAB
• Preparation and Storage: Store at -20 degree C for long term preservation (recommended). Store at 4 degree C for short term use.

Testing Data

(Sample (30 ug of whole cell lysate) A: Hela B: Hep G2 10% SDS PAGE Primary antibody diluted at 1: 1000)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded HS578T xenograft, using CLP1 antibody at 1: 100 dilution.)

Immunofluorescence (IF)

(Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using CLP1 antibody at 1: 200 dilution.)

Related Product Information for anti-HEAB antibody

Polynucleotide kinase that can phosphorylate the 5'-hydroxyl groups of double-stranded RNA (dsRNA), single-stranded RNA (ssRNA), double stranded DNA (dsDNA) and double-stranded DNA:RNA hybrids. dsRNA is phosphorylated more efficiently than dsDNA, and the RNA component of a DNA:RNA hybrid is phosphorylated more efficiently than the DNA component. Appears to have roles in both tRNA splicing and mRNA 3'-end formation. Component of the tRNA splicing endonuclease complex. Phosphorylates the 5'-terminus of the tRNA 3'-exon during tRNA splicing; this phosphorylation event is a prerequisite for the subsequent ligation of the two exon halves and the production of a mature tRNA. Component of the pre-mRNA cleavage complex II (CF-II), which seems to be required for mRNA 3'-end formation. Also phosphorylates the 5'-terminus of exogenously introduced short interfering RNAs (siRNAs), which is a necessary prerequisite for their incorporation into the RNA-induced silencing complex (RISC). However endogenous siRNAs and microRNAs (miRNAs) that are produced by the cleavage of dsRNA precursors by DICER1 already contain a 5'-phosphate group, so this protein may be dispensible for normal RNA-mediated gene silencing.

Product Categories/Family for anti-HEAB antibody

Total protein Ab

NCBI and Uniprot Product Information

• NCBI GI #: 12653353
• NCBI GeneID: 10978
• Molecular Weight: 40,696 Da
• NCBI Official Full Name: CLP1, cleavage and polyadenylation factor I subunit, homolog (S. cerevisiae)
• NCBI Official Synonym Full Names: cleavage and polyadenylation factor I subunit 1
• NCBI Official Symbol: CLP1
• NCBI Official Synonym Symbols: HEAB; hClp1
• NCBI Protein Information: polyribonucleotide 5'-hydroxyl-kinase Clp1
• UniProt Protein Name: Polyribonucleotide 5'-hydroxyl-kinase Clp1
• UniProt Gene Name: CLP1
• UniProt Entry Name: CLP1_HUMAN

NCBI Description

This gene encodes a member of the Clp1 family. The encoded protein is a multifunctional kinase which is a component of the tRNA splicing endonuclease complex and a component of the pre-mRNA cleavage complex II. This protein is implicated in tRNA, mRNA, and siRNA maturation. Mutations in this gene are associated with pontocerebellar hypoplasia type 10 (PCH10). Alternatively splice transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2014]

Uniprot Description

HEAB: Polynucleotide kinase that can phosphorylate the 5'- hydroxyl groups of double-stranded RNA (dsRNA), single-stranded RNA (ssRNA), double stranded DNA (dsDNA) and double-stranded DNA:RNA hybrids. dsRNA is phosphorylated more efficiently than dsDNA, and the RNA component of a DNA:RNA hybrid is phosphorylated more efficiently than the DNA component. Appears to have roles in both tRNA splicing and mRNA 3'-end formation. Component of the tRNA splicing endonuclease complex. Phosphorylates the 5'-terminus of the tRNA 3'-exon during tRNA splicing; this phosphorylation event is a prerequisite for the subsequent ligation of the two exon halves and the production of a mature tRNA. Component of the pre-mRNA cleavage complex II (CF-II), which seems to be required for mRNA 3'-end formation. Also phosphorylates the 5'-terminus of exogenously introduced short interfering RNAs (siRNAs), which is a necessary prerequisite for their incorporation into the RNA- induced silencing complex (RISC). However, endogenous siRNAs and microRNAs (miRNAs) that are produced by the cleavage of dsRNA precursors by DICER1 already contain a 5'-phosphate group, so this protein may be dispensible for normal RNA-mediated gene silencing. Belongs to the Clp1 family. Clp1 subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 2.7.1.78; RNA-binding; RNA splicing

Chromosomal Location of Human Ortholog: 11q12

Cellular Component: nucleoplasm; cytoplasm; nucleus; mRNA cleavage factor complex; tRNA-intron endonuclease complex

Molecular Function: ATP-dependent polydeoxyribonucleotide 5'-hydroxyl-kinase activity; polydeoxyribonucleotide kinase activity; ATP-dependent polyribonucleotide 5'-hydroxyl-kinase activity; ATP binding

Biological Process: transcription from RNA polymerase II promoter; RNA interference, siRNA loading onto RISC; RNA splicing; RNA interference, targeting of mRNA for destruction; mRNA polyadenylation; nuclear mRNA splicing, via spliceosome; cerebellar cortex development; tRNA splicing; gene expression; mRNA 3'-end processing; termination of RNA polymerase II transcription; phosphorylation; mRNA cleavage

Disease: Pontocerebellar Hypoplasia, Type 10