Mouse Desmin ELISA Kit | Des elisa kit

Mouse Desmin ELISA Kit

Cat. Num. AAA760743

• Gene Names: DES; CSM1; CSM2; LGMD2R
• Reactivity: Mouse
• Synonyms: Desmin; Mouse Desmin ELISA Kit; Des/CMD1I/DES/CMD1IFLJ41013/CSM1/CSM2/desmin/FLJ12025/FLJ39719/FLJ41793/intermediate filament protein; Des elisa kit

• Reactivity: Mouse
• Sequence Length: 470

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 0.156-10ng/ml
• Sensitivity: 0.094ng/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Standard Curve

Related Product Information for Des elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. anti-Mouse Des antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Mouse Des antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Mouse Des amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Mouse Des can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 55749932
• NCBI GeneID: 1674
• NCBI Accession #: NP_001918.3
• NCBI GenBank Nucleotide #: NM_001927.3
• Molecular Weight: 53,536 Da
• NCBI Official Full Name: desmin
• NCBI Official Synonym Full Names: desmin
• NCBI Official Symbol: DES
• NCBI Official Synonym Symbols: CSM1; CSM2; LGMD2R
• NCBI Protein Information: desmin
• UniProt Protein Name: Desmin
• Protein Family: Desmin
• UniProt Gene Name: DES
• UniProt Entry Name: DESM_HUMAN

NCBI Description

This gene encodes a muscle-specific class III intermediate filament. Homopolymers of this protein form a stable intracytoplasmic filamentous network connecting myofibrils to each other and to the plasma membrane. Mutations in this gene are associated with desmin-related myopathy, a familial cardiac and skeletal myopathy (CSM), and with distal myopathies. [provided by RefSeq, Jul 2008]

Uniprot Description

desmin: a class-III intermediate filaments found in muscle cells. In adult striated muscle they form a fibrous network connecting myofibrils to each other and to the plasma membrane from the periphery of the Z-line structures. Mutations in this gene are associated with desmin-related myopathy, a familial cardiac and skeletal myopathy (CSM), and with distal myopathies.

Protein type: Cytoskeletal; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 2q35

Cellular Component: cytosol; fascia adherens; intermediate filament; intermediate filament cytoskeleton; neuromuscular junction; sarcolemma; Z disc

Molecular Function: cytoskeletal protein binding; identical protein binding; protein binding; structural constituent of cytoskeleton

Biological Process: cytoskeleton organization and biogenesis; intermediate filament organization; muscle contraction; muscle filament sliding; regulation of heart contraction

Disease: Cardiomyopathy, Dilated, 1i; Muscular Dystrophy, Limb-girdle, Type 2r; Myopathy, Myofibrillar, 1; Scapuloperoneal Syndrome, Neurogenic, Kaeser Type