Rabbit CLP1 Polyclonal Antibody | anti-CLP1 antibody

CLP1 Antibody (C-term)

Cat. Num. AAA9211576

• Gene Names: CLP1; HEAB; hClp1
• Reactivity: Human (Predicted Reactivity: Bovine, Chicken, Monkey, Mouse, Rat)
• Applications: Western Blot, ELISA
• Purity: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Synonyms: CLP1; Polyclonal Antibody; CLP1 Antibody (C-term); Polyribonucleotide 5'-hydroxyl-kinase Clp1 {ECO:0000255|HAMAP-Rule:MF_03035}; 27178 {ECO:0000255|HAMAP-Rule:MF_03035}; Polyadenylation factor Clp1 {ECO:0000255|HAMAP-Rule:MF_03035}; Polynucleotide kinase Clp1 {ECO:0000255|HAMAP-Rule:MF_03035}; Pre-mRNA cleavage complex II protein Clp1 {ECO:0000255|HAMAP-Rule:MF_03035}; CLP1 {ECO:0000255|HAMAP-Rule:MF_03035}; HEAB; anti-CLP1 antibody

• Host: Rabbit
• Reactivity: Human (Predicted Reactivity: Bovine, Chicken, Monkey, Mouse, Rat)
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Specificity: This CLP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 308-336 amino acids from the C-terminal region of human CLP1.
• Purity/Purification: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Concentration: Vial Concentration: 0.5 (varies by lot)
• Sequence Positions: 308-336
• Sequence Length: 361

• Applicable Applications for anti-CLP1 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB~~1:1000
• Antigen Type: Synthetic Peptide
• Antigen Source: HUMAN
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(CLP1 Antibody (C-term) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the CLP1 antibody detected the CLP1 protein (arrow).)

Related Product Information for anti-CLP1 antibody

Polynucleotide kinase that can phosphorylate the 5'-hydroxyl groups of double-stranded RNA (dsRNA), single-stranded RNA (ssRNA), double stranded DNA (dsDNA) and double-stranded DNA:RNA hybrids. dsRNA is phosphorylated more efficiently than dsDNA, and the RNA component of a DNA:RNA hybrid is phosphorylated more efficiently than the DNA component. Appears to have roles in both tRNA splicing and mRNA 3'-end formation. Component of the tRNA splicing endonuclease complex. Phosphorylates the 5'-terminus of the tRNA 3'-exon during tRNA splicing; this phosphorylation event is a prerequisite for the subsequent ligation of the two exon halves and the production of a mature tRNA. Component of the pre-mRNA cleavage complex II (CF-II), which seems to be required for mRNA 3'-end formation. Also phosphorylates the 5'-terminus of exogenously introduced short interfering RNAs (siRNAs), which is a necessary prerequisite for their incorporation into the RNA-induced silencing complex (RISC). However endogenous siRNAs and microRNAs (miRNAs) that are produced by the cleavage of dsRNA precursors by DICER1 already contain a 5'-phosphate group, so this protein may be dispensible for normal RNA-mediated gene silencing.

References

Ramirez, A., et al. RNA 14(9):1737-1745(2008)
Weitzer, S., et al. Nature 447(7141):222-226(2007)
Paushkin, S.V., et al. Cell 117(3):311-321(2004)
Zhou, Z., et al. Nature 419(6903):182-185(2002)
de Vries, H., et al. EMBO J. 19(21):5895-5904(2000)

NCBI and Uniprot Product Information

• NCBI GI #: 217272859
• NCBI GeneID: 10978
• NCBI Accession #: NP_001136069.1; NP_006822.1
• NCBI GenBank Nucleotide #: NM_001142597.1
• UniProt Accession #: Q92989; B2R7J6; B4DTI8
• Molecular Weight: 47646
• NCBI Official Full Name: polyribonucleotide 5'-hydroxyl-kinase Clp1 isoform 2
• NCBI Official Synonym Full Names: cleavage and polyadenylation factor I subunit 1
• NCBI Official Symbol: CLP1
• NCBI Official Synonym Symbols: HEAB; hClp1
• NCBI Protein Information: polyribonucleotide 5'-hydroxyl-kinase Clp1
• UniProt Protein Name: Polyribonucleotide 5'-hydroxyl-kinase Clp1
• Protein Family: Tyrosine-protein phosphatase
• UniProt Gene Name: CLP1
• UniProt Entry Name: CLP1_HUMAN

NCBI Description

This gene encodes a member of the Clp1 family. The encoded protein is a multifunctional kinase which is a component of the tRNA splicing endonuclease complex and a component of the pre-mRNA cleavage complex II. This protein is implicated in tRNA, mRNA, and siRNA maturation. Mutations in this gene are associated with pontocerebellar hypoplasia type 10 (PCH10). Alternatively splice transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2014]

Uniprot Description

HEAB: Polynucleotide kinase that can phosphorylate the 5'- hydroxyl groups of double-stranded RNA (dsRNA), single-stranded RNA (ssRNA), double stranded DNA (dsDNA) and double-stranded DNA:RNA hybrids. dsRNA is phosphorylated more efficiently than dsDNA, and the RNA component of a DNA:RNA hybrid is phosphorylated more efficiently than the DNA component. Appears to have roles in both tRNA splicing and mRNA 3'-end formation. Component of the tRNA splicing endonuclease complex. Phosphorylates the 5'-terminus of the tRNA 3'-exon during tRNA splicing; this phosphorylation event is a prerequisite for the subsequent ligation of the two exon halves and the production of a mature tRNA. Component of the pre-mRNA cleavage complex II (CF-II), which seems to be required for mRNA 3'-end formation. Also phosphorylates the 5'-terminus of exogenously introduced short interfering RNAs (siRNAs), which is a necessary prerequisite for their incorporation into the RNA- induced silencing complex (RISC). However, endogenous siRNAs and microRNAs (miRNAs) that are produced by the cleavage of dsRNA precursors by DICER1 already contain a 5'-phosphate group, so this protein may be dispensible for normal RNA-mediated gene silencing. Belongs to the Clp1 family. Clp1 subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: RNA-binding; RNA splicing; EC 2.7.1.78

Chromosomal Location of Human Ortholog: 11q12

Cellular Component: nucleoplasm; cytoplasm; nucleus; tRNA-intron endonuclease complex; mRNA cleavage factor complex

Molecular Function: ATP-dependent polydeoxyribonucleotide 5'-hydroxyl-kinase activity; polydeoxyribonucleotide kinase activity; ATP-dependent polyribonucleotide 5'-hydroxyl-kinase activity; ATP binding

Biological Process: nuclear mRNA splicing, via spliceosome; transcription from RNA polymerase II promoter; cerebellar cortex development; tRNA splicing; RNA interference, siRNA loading onto RISC; RNA splicing; RNA interference, targeting of mRNA for destruction; mRNA polyadenylation; gene expression; mRNA 3'-end processing; phosphorylation; termination of RNA polymerase II transcription; mRNA cleavage

Disease: Pontocerebellar Hypoplasia, Type 10