Cdk4 blocking peptide

Mouse Cdk4 Antibody (C-term) Blocking Peptide

• Gene Names: Cdk4; Crk3
• Synonyms: Cdk4; Mouse Cdk4 Antibody (C-term) Blocking Peptide; Cyclin-dependent kinase 4; CRK3; Cell division protein kinase 4; PSK-J3; Crk3; Cdk4 blocking peptide

• Form/Format: Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.
• Sequence Length: 303

• Cellular Location: Cytoplasm. Nucleus. Membrane. Note: Cytoplasmic when non- complexed. Forms a cyclin D-CDK4 complex in the cytoplasm as cells progress through G(1) phase. The complex accumulates on the nuclear membrane and enters the nucleus on transition from G(1) to S phase. Also present in nucleoli and heterochromatin lumps Colocalizes with RB1 after release into the nucleus (By similarity).
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Related Product Information for Cdk4 blocking peptide

Ser/Thr-kinase component of cyclin D-CDK4 (DC) complexes that phosphorylate and inhibit members of the retinoblastoma (RB) protein family including RB1 and regulate the cell-cycle during G(1)/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complexes and the subsequent transcription of E2F target genes which are responsible for the progression through the G(1) phase. Hypophosphorylates RB1 in early G(1) phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Also phosphorylates SMAD3 in a cell-cycle-dependent manner and represses its transcriptional activity. Component of the ternary complex, cyclin D/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex (By similarity).

NCBI and Uniprot Product Information

• NCBI GI #: 231727
• NCBI GeneID: 12567
• UniProt Accession #: P30285
• Molecular Weight: 33,751 Da
• NCBI Official Full Name: Cyclin-dependent kinase 4
• NCBI Official Synonym Full Names: cyclin-dependent kinase 4
• NCBI Official Symbol: Cdk4
• NCBI Official Synonym Symbols: Crk3
• NCBI Protein Information: cyclin-dependent kinase 4
• UniProt Protein Name: Cyclin-dependent kinase 4
• Protein Family: Cyclin-dependent kinase
• UniProt Gene Name: Cdk4
• UniProt Synonym Gene Names: Crk3
• UniProt Entry Name: CDK4_MOUSE

Uniprot Description

CDK4: a protein kinase of the CDK family that is important for cell cycle G1 phase progression. Its activity is restricted to the G1-S phase. Controlled by the regulatory subunits D-type cyclins and CDK inhibitor p16(INK4a). Phosphorylates the retinoblastoma gene product (Rb). Point mutations found in somatic and familial melanoma. Amplified in sarcomas, glioma and lymphoma. Amplified, methylated or deleted in head and neck squamous cell carcinoma. Overexpression drives epithelial tumors in mice. Disruption makes mice resistant to cancer. Inhibitor: PD332991.

Protein type: Protein kinase, Ser/Thr (non-receptor); Kinase, protein; Protein kinase, CMGC; EC 2.7.11.22; Cell cycle regulation; CMGC group; CDK family; CDK4 subfamily; CDK/CDK4 subfamily

Cellular Component: chromatin; cyclin-dependent protein kinase holoenzyme complex; cytosol; nuclear membrane; nucleolus; nucleus; perinuclear region of cytoplasm; tight junction; transcription factor complex

Molecular Function: cyclin binding; cyclin-dependent protein kinase activity; kinase activity; protein binding; protein complex binding; protein kinase activity

Biological Process: G1/S transition of mitotic cell cycle; positive regulation of apoptosis; positive regulation of cell proliferation; positive regulation of cell size; positive regulation of fibroblast proliferation; positive regulation of translation; protein amino acid phosphorylation; regulation of cell cycle; regulation of cell proliferation; regulation of gene expression; response to drug; response to lead ion; response to organic substance; response to testosterone stimulus; signal transduction