Chicken anti-Human, Mouse Nucleolar Protein Family A, Member 1 Polyclonal Antibody | anti-GAR1 antibody

Nucleolar Protein Family A, Member 1 (GAR1 Protein, NOLA1)

Cat. Num. AAA627756

• Gene Names: GAR1; NOLA1
• Reactivity: Human, Mouse
• Applications: ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: Nucleolar Protein Family A; Member 1; Polyclonal Antibody; Member 1 (GAR1 Protein; NOLA1); Anti -Nucleolar Protein Family A; anti-GAR1 antibody

• Host: Chicken
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgY
• Specificity: Recognizes human Nucleolar Protein Family A, Member 1.
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Glycerol free.

• Applicable Applications for anti-GAR1 antibody: ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC)
• Application Notes: Suitable for use in ELISA, Immunohistochemistry, Immunocytochemistry and Western Blot.; Dilution: Western Blot: 1:250; Immunohistochemistry: 1:100; Immunocytochemistry: 1:100
• Immunogen: Synthetic peptide corresponding to aa1-217 Protein GI Number: 15011916 Protein Accession #: NP_127460.1
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Western Blot (WB)

(Western Blot: E coli-derived fusion protein as test antigen. Affinity-purified IgY dilution: 1:2000, Goat anti-IgY-HRP dilution: 1:1000. Colorimetric method for signal development.)

Related Product Information for anti-GAR1 antibody

The gene that encodes this protein is a member of the H/ACA snoRNPs (small nucleolar ribonucleoproteins) gene family. snoRNPs are involved in various aspects of rRNA processing and modification and have been classified into two families: C/D and H/ACA. The H/ACA snoRNPs also include the DKC1, NOLA2 and NOLA3 proteins. These four H/ACA snoRNP proteins localize to the dense fibrillar components of nucleoli and to coiled (Cajal) bodies in the nucleus. Both 18S rRNA production and rRNA pseudouridylation are impaired if any one of the four proteins is depleted. These four H/ACA snoRNP proteins are also components of the telomerase complex. The encoded protein of this gene contains two glycine- and arginine-rich domains and is related to Saccharomyces cerevisiae Gar1p.

Product Categories/Family for anti-GAR1 antibody

Antibodies; Abs to Nuclear Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 15011916
• NCBI GeneID: 54433
• NCBI Accession #: NP_127460.1
• NCBI GenBank Nucleotide #: NM_032993.2
• UniProt Accession #: Q9NY12; Q5MJQ2
• Molecular Weight: 22,348 Da
• NCBI Official Full Name: H/ACA ribonucleoprotein complex subunit 1
• NCBI Official Synonym Full Names: GAR1 ribonucleoprotein homolog (yeast)
• NCBI Official Symbol: GAR1
• NCBI Official Synonym Symbols: NOLA1
• NCBI Protein Information: H/ACA ribonucleoprotein complex subunit 1; OTTHUMP00000219723; OTTHUMP00000219724; OTTHUMP00000219725; snoRNP protein GAR1; nucleolar protein family A member 1; nucleolar protein family A, member 1 (H/ACA small nucleolar RNPs)
• UniProt Protein Name: H/ACA ribonucleoprotein complex subunit 1
• Protein Family: D-galacturonate reductase
• UniProt Gene Name: GAR1
• UniProt Synonym Gene Names: NOLA1
• UniProt Entry Name: GAR1_HUMAN

NCBI Description

This gene is a member of the H/ACA snoRNPs (small nucleolar ribonucleoproteins) gene family. snoRNPs are involved in various aspects of rRNA processing and modification and have been classified into two families: C/D and H/ACA. The H/ACA snoRNPs also include the DKC1, NOLA2 and NOLA3 proteins. These four H/ACA snoRNP proteins localize to the dense fibrillar components of nucleoli and to coiled (Cajal) bodies in the nucleus. Both 18S rRNA production and rRNA pseudouridylation are impaired if any one of the four proteins is depleted. These four H/ACA snoRNP proteins are also components of the telomerase complex. The encoded protein of this gene contains two glycine- and arginine-rich domains and is related to Saccharomyces cerevisiae Gar1p. Two splice variants have been found for this gene. [provided by RefSeq]

Uniprot Description

NOLA1: Required for ribosome biogenesis and telomere maintenance. Part of the H/ACA small nucleolar ribonucleoprotein (H/ACA snoRNP) complex, which catalyzes pseudouridylation of rRNA. This involves the isomerization of uridine such that the ribose is subsequently attached to C5, instead of the normal N1. Each rRNA can contain up to 100 pseudouridine (psi) residues, which may serve to stabilize the conformation of rRNAs. May also be required for correct processing or intranuclear trafficking of TERC, the RNA component of the telomerase reverse transcriptase (TERT) holoenzyme. Belongs to the GAR1 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Nucleolus; RNA-binding

Chromosomal Location of Human Ortholog: 4q25

Cellular Component: Cajal body; nucleolus; nucleus

Molecular Function: cation channel activity

Biological Process: pseudouridine synthesis; cation transport; rRNA processing