Monster Block ELISA Blocking Buffer
Monster Block ELISA Blocking Buffer, Non-Mammalian
Synonyms
Monster Block ELISA Blocking Buffer; Non-Mammalian; Monster Block ELISA Blocking Buffer blocking buffer
Form/Format
Supplied at 1X.
Application Notes
1. Coat antibody or antigen onto the ELISA plate using ICT's Antibody Coating Buffer or Antigen Coating Buffer.
2. Incubate 8-24 hours at room temperature (RT).
3. Aspirate the coating solution.
4. Wash each well twice with ICT's ELISA Wash Buffer.
5. Block the uncoated regions of the ELISA plate by pipetting 300-400 uL of blocking buffer into each well. Always use an equal or greater volume of blocking buffer than was used for the coating buffer solution.
6. Incubate 8-24 hours at RT. For best blocking, incubate overnight at RT.
7. Aspirate the blocking buffer; do not wash.
8. Run the assay immediately, or dry the plate for long-term storage and seal in a foil storage bag with a desiccant pack. Store dried and packaged plates at 2-8 degree C.
2. Incubate 8-24 hours at room temperature (RT).
3. Aspirate the coating solution.
4. Wash each well twice with ICT's ELISA Wash Buffer.
5. Block the uncoated regions of the ELISA plate by pipetting 300-400 uL of blocking buffer into each well. Always use an equal or greater volume of blocking buffer than was used for the coating buffer solution.
6. Incubate 8-24 hours at RT. For best blocking, incubate overnight at RT.
7. Aspirate the blocking buffer; do not wash.
8. Run the assay immediately, or dry the plate for long-term storage and seal in a foil storage bag with a desiccant pack. Store dried and packaged plates at 2-8 degree C.
pH
7.4 at 1X
Contains
Non-Mammalian Protein Blocking Agents.
Preparation and Storage
Store at 2-8 degree C.
Related Product Information for Monster Block ELISA Blocking Buffer blocking buffer
Description: Monster Block ELISA Blocking Buffer is a high strength blocking buffer designed to address high background problems in antigen-down and sandwich immunoassays. Using a heterogenous mixture of non-mammalian blocking agents, Monster Block reduces non-specific binding and stabilizes proteins.
Background: Monster Block provides a high degree of blocking efficiency and protein stabilization through the use of non-mammalian protein blocking agents and stabilizers. The heterogeneous composition of this unique ELISA blocking buffer efficiently blocks the uncoated regions of the microtiter plate without the use of mammalian protein additives. This buffer is best for assays that will not tolerate conventional mammalian blocking buffers or immunoassays with high background problems. In antigen-down ELISA formats, the use of Monster Block reduces the risk of endogenous antibodies in the sample reacting with the proteins used to block the plate, making Monster Block particularly useful when testing human, swine, and bovine sera.
Monster Block's heterogenous mixture of nonmammalian blocking agents minimizes non specific binding interactions during the immunoassay process, reducing ELISA background noise and increasing specific signal. In addition, the non-mammalian-based Monster Block formulation is antigenically foreign to most mammalian immune systems, reducing the chance of cross-reactivity with anti-isotype detection antibodies.
This buffer also provides a microhydrated environment to stabilize the dried, coated protein during long-term storage for improved retention of antigen epitope and antibody binding activity. Also important when preparing batches for storage is Monster Block's antimicrobial agent, which allows for stable room temperature blocking and long-term refrigerated storage of dried plates.
Background: Monster Block provides a high degree of blocking efficiency and protein stabilization through the use of non-mammalian protein blocking agents and stabilizers. The heterogeneous composition of this unique ELISA blocking buffer efficiently blocks the uncoated regions of the microtiter plate without the use of mammalian protein additives. This buffer is best for assays that will not tolerate conventional mammalian blocking buffers or immunoassays with high background problems. In antigen-down ELISA formats, the use of Monster Block reduces the risk of endogenous antibodies in the sample reacting with the proteins used to block the plate, making Monster Block particularly useful when testing human, swine, and bovine sera.
Monster Block's heterogenous mixture of nonmammalian blocking agents minimizes non specific binding interactions during the immunoassay process, reducing ELISA background noise and increasing specific signal. In addition, the non-mammalian-based Monster Block formulation is antigenically foreign to most mammalian immune systems, reducing the chance of cross-reactivity with anti-isotype detection antibodies.
This buffer also provides a microhydrated environment to stabilize the dried, coated protein during long-term storage for improved retention of antigen epitope and antibody binding activity. Also important when preparing batches for storage is Monster Block's antimicrobial agent, which allows for stable room temperature blocking and long-term refrigerated storage of dried plates.
Product Categories/Family for Monster Block ELISA Blocking Buffer blocking buffer
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Product Notes
The Monster Block ELISA Blocking Buffer (Catalog #AAA258394) is a Blocking Buffer and is intended for research purposes only. The product is available for immediate purchase. 1. Coat antibody or antigen onto the ELISA plate using ICT's Antibody Coating Buffer or Antigen Coating Buffer. 2. Incubate 8-24 hours at room temperature (RT). 3. Aspirate the coating solution. 4. Wash each well twice with ICT's ELISA Wash Buffer. 5. Block the uncoated regions of the ELISA plate by pipetting 300-400 uL of blocking buffer into each well. Always use an equal or greater volume of blocking buffer than was used for the coating buffer solution. 6. Incubate 8-24 hours at RT. For best blocking, incubate overnight at RT. 7. Aspirate the blocking buffer; do not wash. 8. Run the assay immediately, or dry the plate for long-term storage and seal in a foil storage bag with a desiccant pack. Store dried and packaged plates at 2-8 degree C. Researchers should empirically determine the suitability of the Monster Block ELISA Blocking Buffer for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Monster Block ELISA Blocking Buffer, Blocking Buffer" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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