Testing Data
HRP Conjugate Stabilizer Reagent
HRP Conjugate Stabilizer, Mammalian, 1X
Synonyms
HRP Conjugate Stabilizer; Mammalian; 1X; HRP Conjugate Stabilizer reagent
Form/Format
Supplied at 1X.
Application Notes
1. Protect the HRP Conjugate Stabilizer from light. This may be done by covering the container with aluminum foil.
2. Add the HRP-conjugated protein at the target concentration to HRP Conjugate Stabilizer. For example, if diluting an HRP-conjugated antibody 1:5000, add 10 uL conjugate stock to 49.99 mL HRP Conjugate Stabilizer.
3. Mix for 15 minutes.
4. Use the diluted HRP conjugate in the immunoassay.
5. Store remaining HRP conjugate solution at 2-8 degree C. Protect from direct exposure to light.
2. Add the HRP-conjugated protein at the target concentration to HRP Conjugate Stabilizer. For example, if diluting an HRP-conjugated antibody 1:5000, add 10 uL conjugate stock to 49.99 mL HRP Conjugate Stabilizer.
3. Mix for 15 minutes.
4. Use the diluted HRP conjugate in the immunoassay.
5. Store remaining HRP conjugate solution at 2-8 degree C. Protect from direct exposure to light.
pH
7.4 at 1X
Contains
Mammalian Proteins.
Preparation and Storage
Store at 2-8 degree C.
Testing Data
Testing Data
Related Product Information for HRP Conjugate Stabilizer reagent
Description: Preserve activity of HRP conjugates and reduce background signal in immunoassays using HRP detection systems. HRP Conjugate Stabilizer is used to preserve concentrated stock conjugates, reconstitute lyophilized conjugates, and dilute antibody-HRP conjugates to their useful working titer in ELISAs and immunology-based techniques.
Background: HRP Conjugate Stabilizer, Mammalian, 1X preserves the activity of HRP-protein conjugates during storage, reconstitution, and immunoassay use. This HRP stabilizer can be used to store concentrated stock HRP conjugates, reconstitute lyophilized conjugates, and dilute HRP conjugated antibodies to their useful working titer in ELISA tests and immunology-based techniques. HRP Conjugate Stabilizer is especially suited for ELISAs using anti-IgG HRP conjugates within traditional antigen-down or antibody sandwich formats. Additional components may be added to HRP Conjugate Stabilizer, Mammalian, 1X to optimize it for particular ELISA format compositions or for use in other immunological techniques. Antimicrobial agents are included to retard bacterial growth.
HRP Conjugate Stabilizer, Mammalian, 1X preserves the functional integrity of the horseradish peroxidase enzyme and immunoglobulin components of stock conjugates upon reconstitution or dilution to its working concentration for ELISA or other immunology-based techniques. Its proprietary formula also stabilizes the HRP conjugate during storage and enhances the bond between detection HRP conjugated antibody and target antigen or antibody.
Mammalian proteins and salt additives provide structural support to both the immunoglobulin and HRP components of the conjugate, preserving enzymatic and IgG activity and stabilizing the conjugate bond.
HRP Conjugate Stabilizer, Mammalian, 1X inhibits the HRP conjugate from binding to nonspecific serum proteins in the sample, thereby reducing conjugate bridging on the ELISA plate surface and reducing non-specific background noise.
Background: HRP Conjugate Stabilizer, Mammalian, 1X preserves the activity of HRP-protein conjugates during storage, reconstitution, and immunoassay use. This HRP stabilizer can be used to store concentrated stock HRP conjugates, reconstitute lyophilized conjugates, and dilute HRP conjugated antibodies to their useful working titer in ELISA tests and immunology-based techniques. HRP Conjugate Stabilizer is especially suited for ELISAs using anti-IgG HRP conjugates within traditional antigen-down or antibody sandwich formats. Additional components may be added to HRP Conjugate Stabilizer, Mammalian, 1X to optimize it for particular ELISA format compositions or for use in other immunological techniques. Antimicrobial agents are included to retard bacterial growth.
HRP Conjugate Stabilizer, Mammalian, 1X preserves the functional integrity of the horseradish peroxidase enzyme and immunoglobulin components of stock conjugates upon reconstitution or dilution to its working concentration for ELISA or other immunology-based techniques. Its proprietary formula also stabilizes the HRP conjugate during storage and enhances the bond between detection HRP conjugated antibody and target antigen or antibody.
Mammalian proteins and salt additives provide structural support to both the immunoglobulin and HRP components of the conjugate, preserving enzymatic and IgG activity and stabilizing the conjugate bond.
HRP Conjugate Stabilizer, Mammalian, 1X inhibits the HRP conjugate from binding to nonspecific serum proteins in the sample, thereby reducing conjugate bridging on the ELISA plate surface and reducing non-specific background noise.
Product Categories/Family for HRP Conjugate Stabilizer reagent
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Product Notes
The HRP Conjugate Stabilizer (Catalog #AAA258399) is a Reagent and is intended for research purposes only. The product is available for immediate purchase. 1. Protect the HRP Conjugate Stabilizer from light. This may be done by covering the container with aluminum foil. 2. Add the HRP-conjugated protein at the target concentration to HRP Conjugate Stabilizer. For example, if diluting an HRP-conjugated antibody 1:5000, add 10 uL conjugate stock to 49.99 mL HRP Conjugate Stabilizer. 3. Mix for 15 minutes. 4. Use the diluted HRP conjugate in the immunoassay. 5. Store remaining HRP conjugate solution at 2-8 degree C. Protect from direct exposure to light. . Researchers should empirically determine the suitability of the HRP Conjugate Stabilizer for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HRP Conjugate Stabilizer, Reagent" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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