Testing Data
Alternative Block ELISA Blocking Buffer
Alternative Block ELISA Blocking Buffer, Synthetic
Synonyms
Alternative Block ELISA Blocking Buffer; Synthetic; Alternative Block ELISA Blocking Buffer blocking buffer
Application Notes
1. Coat antibody or antigen onto the ELISA plate using ICT's Antibody Coating Buffer or Antigen Coating Buffer.
2. Incubate 8-24 hours at room temperature (RT).
3. Aspirate the coating solution.
4. Wash each well twice with ELISA Wash Buffer.
5. Block the uncoated regions of the ELISA plate by pipetting 300-400 uL of blocking buffer into each well. Always use an equal or greater volume of blocking buffer than was used for the coating buffer solution.
6. Incubate 8-24 hours.
7. Aspirate the blocking buffer; do not wash.
8. Run the assay immediately, or dry the plate for long-term storage and seal in a foil bag (MBS258081) with a desiccant pack (MBS150680).
2. Incubate 8-24 hours at room temperature (RT).
3. Aspirate the coating solution.
4. Wash each well twice with ELISA Wash Buffer.
5. Block the uncoated regions of the ELISA plate by pipetting 300-400 uL of blocking buffer into each well. Always use an equal or greater volume of blocking buffer than was used for the coating buffer solution.
6. Incubate 8-24 hours.
7. Aspirate the blocking buffer; do not wash.
8. Run the assay immediately, or dry the plate for long-term storage and seal in a foil bag (MBS258081) with a desiccant pack (MBS150680).
Specifications
Clear liquid
1x ready to use
pH 7.1-7.6
1x ready to use
pH 7.1-7.6
Usage
Contains <0.1% sodium azide.
Preparation and Storage
Store at 2-8°C for 24 months. Store at Room Temperature for 1 week.
Testing Data
Testing Data
Related Product Information for Alternative Block ELISA Blocking Buffer blocking buffer
Alternative Block is a protein-free, detergent-free ELISA blocking buffer. This unique blocking buffer contains a heterogeneous mixture of proprietary blockers and synthetic stabilizers that block the uncoated regions of the plate without the use of conventional cross-reactive protein additives or detergents.
Alternative Block minimizes non-specific binding interactions and reduces background noise. It also stabilizes the coated protein during long-term storage by providing a microhydrated environment for improved retention of antigen epitope and antibody binding activity. Room temperature blocking of the plate and long-term refrigerated storage of dried plates are made possible by an antimicrobial component.
Alternative Block is suitable for use in most monoclonal and polyclonal antibody capture ELISA tests (also known as sandwich ELISAs) and peptide/protein antigen-down ELISAs. When preparing plates, the antibody or antigen is typically coated using 50-200 uL of coating solution per well. After coating, plates are washed to remove unbound proteins and then blocked using a larger volume of blocking buffer than was used for coating, such as 300 uL per well. This ensures that all uncoated regions inside the well are blocked. A 96-well plate blocked using this method will require 28.8 mL of blocking solution. Allow 10% extra blocking buffer to account for losses during pipetting.
Alternative Block minimizes non-specific binding interactions and reduces background noise. It also stabilizes the coated protein during long-term storage by providing a microhydrated environment for improved retention of antigen epitope and antibody binding activity. Room temperature blocking of the plate and long-term refrigerated storage of dried plates are made possible by an antimicrobial component.
Alternative Block is suitable for use in most monoclonal and polyclonal antibody capture ELISA tests (also known as sandwich ELISAs) and peptide/protein antigen-down ELISAs. When preparing plates, the antibody or antigen is typically coated using 50-200 uL of coating solution per well. After coating, plates are washed to remove unbound proteins and then blocked using a larger volume of blocking buffer than was used for coating, such as 300 uL per well. This ensures that all uncoated regions inside the well are blocked. A 96-well plate blocked using this method will require 28.8 mL of blocking solution. Allow 10% extra blocking buffer to account for losses during pipetting.
Product Categories/Family for Alternative Block ELISA Blocking Buffer blocking buffer
Similar Products
Product Notes
The Alternative Block ELISA Blocking Buffer (Catalog #AAA258395) is a Blocking Buffer and is intended for research purposes only. The product is available for immediate purchase. 1. Coat antibody or antigen onto the ELISA plate using ICT's Antibody Coating Buffer or Antigen Coating Buffer. 2. Incubate 8-24 hours at room temperature (RT). 3. Aspirate the coating solution. 4. Wash each well twice with ELISA Wash Buffer. 5. Block the uncoated regions of the ELISA plate by pipetting 300-400 uL of blocking buffer into each well. Always use an equal or greater volume of blocking buffer than was used for the coating buffer solution. 6. Incubate 8-24 hours. 7. Aspirate the blocking buffer; do not wash. 8. Run the assay immediately, or dry the plate for long-term storage and seal in a foil bag (MBS258081) with a desiccant pack (MBS150680). Researchers should empirically determine the suitability of the Alternative Block ELISA Blocking Buffer for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Alternative Block ELISA Blocking Buffer, Blocking Buffer" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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