Typical Testing Data/Standard Curve (for reference only)
Human Alpha 1-Antitrypsin ELISA Kit
Alpha 1-Antitrypsin (Human) ELISA Kit
Reactivity
Human
Synonyms
Alpha 1-Antitrypsin; Alpha 1-Antitrypsin (Human) ELISA Kit; Alpha 1-Antitrypsin elisa kit
Reactivity
Human
Specificity
A Sandwich ELISA kit for the quantitative measurement of Alpha 1-Antitrypsin in human plasma; serum and other biological fluids.
Assay Type
Sandwich
Detection Method
Absorbance (450nM)
Sample Type
Plasma, Serum, Other biological fluids
Sensitivity
1.88ng/mL
Precision
Coefficient of variation is <10%
Detection Range
3.13-200ng/mL
Cross-reactivity
No significant cross-reactivity or interference between Human alpha1-AT and analogues was observed.
Preparation and Storage
Store at 4 degree C
Shipping: Gel Pack
Shelf Life: Est.~6 months
Shipping: Gel Pack
Shelf Life: Est.~6 months
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for Alpha 1-Antitrypsin elisa kit
Alpha 1-Antitrypsin is an inhibitor of serine proteases. Its primary target is elastase, but it also has a moderate affinity for plasmin and thrombin. Irreversibly inhibits trypsin, chymotrypsin, and plasminogen activator. The aberrant form inhibits insulin-induced NO synthesis in platelets, decreases coagulation time and has proteolytic activity against insulin and plasmin. Alpha 1-Antitrypsin (Human) ELISA Kit is based on the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human Alpha 1-Antitrypsin. Standards or samples are added to the micro ELISA plate wells that bind to the specific antibody. Then a biotinylated detection antibody specific for Human Alpha 1-Antitrypsin and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. The wells are washed, a TMB substrate solution is added to the wells and blue color develops in proportion to the amount of Alpha 1-Antitrypsin bound. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450nM. The OD value is proportional to the concentration of Human Alpha 1-Antitrypsin. The concentration of Human Alpha 1-Antitrypsinin the samples can be calculated by comparing the OD of the samples to the standard curve.
Product Categories/Family for Alpha 1-Antitrypsin elisa kit
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Product Notes
The Human Alpha 1-Antitrypsin (Catalog #AAA8400537) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA8400537 ELISA Kit recognizes Human Alpha 1-Antitrypsin. It is sometimes possible for the material contained within the vial of "Alpha 1-Antitrypsin, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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