BCL2L1 / Bcl-XL recombinant protein

Recombinant Mouse BCL2L1 / Bcl-XL Protein (aa 1-212, His tag)

Cat. Num. AAA2547215

• Gene Names: Bcl2l1; BclX; Bcl2l; bcl-x; Bcl-XL; Bcl(X)L; bcl2-L-1
• Purity: > 95 % as determined by SDS-PAGE
• Synonyms: BCL2L1 / Bcl-XL; Recombinant Mouse BCL2L1 / Bcl-XL Protein (aa 1-212; His tag); RP23-106A3.4; Bcl (X)L; Bcl-XL; Bcl-Xs; Bcl2l; BclX; MGC113803; MGC99998; bcl-x; BCL2L1 / Bcl-XL recombinant protein

• Host: E Coli
• Purity/Purification: > 95 % as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH 7.5
• Sequence Length: 233

• Application Notes: The recombinant mouse BCL2L1 comprises 222 amino acids and has a calculated molecular mass of 25.2 kDa. It migrates as an approximately 33 kDa band in SDS-PAGE under reducing conditions.
• Predicted N Terminal: Met 1
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -70 degree C

Related Product Information for BCL2L1 / Bcl-XL recombinant protein

Background: B-cell lymphoma-extra large (Bcl-xl) is a transmembrane molecule in the mitochondria. Bcl-xL (BCL2L1), belongs to the Bcl-2 family. Members of the bcl-2 family encode proteins that function either to promote or to inhibit apoptosis. Antiapoptotic members such as Bcl-2 and Bcl-xL prevent PCD in response to a wide variety of stimuli to take part in cancer survival. Conversely, proapoptotic proteins, exemplified by Bax and Bak, can accelerate death and in some instances are sufficient to cause apoptosis independent of additional signals. The crystal and solution structures of a Bcl-2 family member, Bcl-xL is like this: The structures consist of two central, primarily hydrophobic alpha-helices, which are surrounded by amphipathic helices. A 60-residue loop connecting helices alphal and alpha2 was found to be flexible and non-essential for anti-apoptotic activity. Bcl-xL is chareacterized as important factors in autophagy, inhibiting Beclin 1-mediated autophagy by binding to Beclin 1. In addition, Beclin 1, Bcl-2 and Bcl-xL can cooperate with Atg5 or Ca2+ to regulate both autophagy and apoptosis. Bcl-xL is also implicated in anoxia induced cell death. The pathway is initiated by the loss of function of the prosurvival Bcl-2 family members Mcl-1 and Bcl-2 / Bcl-XL, resulting in Bax- or Bak-dependent release of cytochrome c and subsequent caspase-9-dependent cell death. Thus, Bcl-xL, the well-characterized apoptosis guards, appears to be important in cell death.

Description: A DNA sequence encoding the mouse BCL2L1 (Q64373-1) (Met 1-Arg 212) was expressed, with a C-terminal polyhistidine tag.

NCBI and Uniprot Product Information

• NCBI GI #: 577019442
• NCBI GeneID: 12048
• NCBI Accession #: NP_001276645.1
• NCBI GenBank Nucleotide #: NM_001289716.1
• UniProt Accession #: Q64373; O35844; Q60657; Q60658; Q61338
• Molecular Weight: 23,900 Da
• NCBI Official Full Name: bcl-2-like protein 1 isoform a
• NCBI Official Synonym Full Names: BCL2-like 1
• NCBI Official Symbol: Bcl2l1
• NCBI Official Synonym Symbols: BclX; Bcl2l; bcl-x; Bcl-XL; Bcl(X)L; bcl2-L-1
• NCBI Protein Information: bcl-2-like protein 1
• UniProt Protein Name: Bcl-2-like protein 1
• UniProt Gene Name: Bcl2l1
• UniProt Synonym Gene Names: Bcl2l; Bclx; Bcl2-L-1

NCBI Description

This gene encodes a member of the Bcl-2 family of apoptosis regulators. The encoded protein is localized to the inner and outer mitochondrial membranes and regulates the programmed cell death pathway during development and tissue homeostasis. This protein binds to voltage-dependent anion channels in the outer mitochondrial membrane to facilitate the uptake of calcium ions. Mice embryos lacking this gene survived for two weeks and exhibited cell death of immature hematopoietic cells and neurons. Alternative splicing results in multiple transcript variants. Additional alternatively spliced transcript variants of this gene have been described, but their full-length nature is not known. [provided by RefSeq, Jan 2014]

Uniprot Description

Bcl-xL: an antiapoptotic member of the Bcl-2 family. Located at the outer mitochondrial membrane and regulates outer mitochondrial membrane channel (VDAC) opening. VDAC regulates mitochondrial membrane potential, and thus controls the production of reactive oxygen species and release of cytochrome C by mitochondria, both of which are potent inducers of cell apoptosis. Two alternatively spliced isoforms have been reported.

Protein type: Apoptosis; Autophagy; Membrane protein, integral; Mitochondrial

Chromosomal Location of Human Ortholog: 2 75.41 cM|2 H1

Cellular Component: centrosome; cytoplasm; cytosol; intracellular; mitochondrial envelope; mitochondrial membrane; mitochondrial outer membrane; mitochondrion

Molecular Function: BH domain binding; BH3 domain binding; caspase inhibitor activity; protein binding; protein heterodimerization activity; protein homodimerization activity; protein kinase binding; transcription factor binding

Biological Process: cell proliferation; cellular process regulating host cell cycle in response to virus; cytokinesis; DNA damage response, signal transduction resulting in induction of apoptosis; fertilization; germ cell development; growth; in utero embryonic development; male gonad development; mitotic cell cycle checkpoint; negative regulation of apoptosis; negative regulation of neuron apoptosis; neuron apoptosis; ovarian follicle development; positive regulation of apoptosis; positive regulation of cell proliferation; regulation of apoptosis; regulation of mitochondrial membrane permeability; regulation of mitochondrial membrane potential; release of cytochrome c from mitochondria; response to cycloheximide; response to cytokine stimulus; response to radiation; response to virus; spermatogenesis; suppression by virus of host apoptosis