Mouse B-Cell CLL/Lymphoma 2 Like Protein (Bcl2L) ELISA Kit | Bcl2L elisa kit

Mouse B-Cell CLL/Lymphoma 2 Like Protein (Bcl2L) ELISA Kit

Cat. Num. AAA456196

• Gene Names: Bcl2l1; BclX; Bcl2l; bcl-x; Bcl-XL; Bcl(X)L; bcl2-L-1
• Reactivity: Mouse
• Synonyms: B-Cell CLL/Lymphoma 2 Like Protein (Bcl2L); Mouse B-Cell CLL/Lymphoma 2 Like Protein (Bcl2L) ELISA Kit; Bcl2L elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of Bcl2L. No significant cross-reactivity or interference between Bcl2L and analogues was observed.
• Sequence Length: 214

• Samples: Tissue homogenates, cell lysates and other biological fluids.
• Assay Type: Sandwich
• Detection Range: 78.125-5000pg/mL
• Sensitivity: 36.37pg/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Bcl2L were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Bcl2L were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

Typical Standard Curve/Testing Data

Related Product Information for Bcl2L elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of Bcl2L in mouse tissue homogenates, cell lysates and other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to Bcl2L. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to Bcl2L. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Bcl2L, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Bcl2L in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 577019442
• NCBI GeneID: 12048
• NCBI Accession #: NP_001276645.1
• NCBI GenBank Nucleotide #: NM_001289716.1
• UniProt Accession #: Q64373; O35844; Q60657; Q60658; Q61338
• Molecular Weight: 23,900 Da
• NCBI Official Full Name: bcl-2-like protein 1 isoform a
• NCBI Official Synonym Full Names: BCL2-like 1
• NCBI Official Symbol: Bcl2l1
• NCBI Official Synonym Symbols: BclX; Bcl2l; bcl-x; Bcl-XL; Bcl(X)L; bcl2-L-1
• NCBI Protein Information: bcl-2-like protein 1
• UniProt Protein Name: Bcl-2-like protein 1
• UniProt Gene Name: Bcl2l1
• UniProt Synonym Gene Names: Bcl2l; Bclx; Bcl2-L-1

NCBI Description

This gene encodes a member of the Bcl-2 family of apoptosis regulators. The encoded protein is localized to the inner and outer mitochondrial membranes and regulates the programmed cell death pathway during development and tissue homeostasis. This protein binds to voltage-dependent anion channels in the outer mitochondrial membrane to facilitate the uptake of calcium ions. Mice embryos lacking this gene survived for two weeks and exhibited cell death of immature hematopoietic cells and neurons. Alternative splicing results in multiple transcript variants. Additional alternatively spliced transcript variants of this gene have been described, but their full-length nature is not known. [provided by RefSeq, Jan 2014]

Uniprot Description

Potent inhibitor of cell death. Inhibits activation of caspases. Appears to regulate cell death by blocking the voltage-dependent anion channel (VDAC) by binding to it and preventing the release of the caspase activator, CYC1, from the mitochondrial membrane. Also acts as a regulator of G2 checkpoint and progression to cytokinesis during mitosis.