Immunohistochemistry (IHC)
(Human Skin: Formalin-Fixed, Paraffin-Embedded (FFPE))
Rabbit Histone H4 Polyclonal Antibody | anti-HIS4 antibody
Anti-Histone H4 Antibody (Lys16)
Gene Names
HIS4; F8N16.2; F8N16_2; histone H4
Reactivity
HumanPredicted: Mouse, Rat
Applications
ELISA, Immunofluorescence, Immunohistochemistry
Purity
Immunoaffinity Purified
Synonyms
Histone H4; Polyclonal Antibody; Anti-Histone H4 Antibody (Lys16); Mouse Monoclonal (IgG1) to Human Histone H4; anti-HIS4 antibody
Host
Rabbit
Reactivity
Human
Predicted: Mouse, Rat
Predicted: Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
Histone H4 (Acetyl-Lys16) Antibody detects endogenous levels of total Histone H4 protein only when acetylated at lysine16.
Purity/Purification
Immunoaffinity Purified
Form/Format
PBS, pH 7.4, 150 mM sodium chloride, 0.02% sodium azide, 50% glycerol
Sequence Length
103
Applicable Applications for anti-HIS4 antibody
ELISA (EIA), Immunofluorescence (IF), Immunohistochemistry (IHC) Paraffin
Application Notes
ELISA: 1:5000
IF: 1:100-1:500
IHC-P: 1:50
IF: 1:100-1:500
IHC-P: 1:50
Immunogen
Synthetic peptide from human Histone H4 around the acetylated site of Lys16 (aa1-50).
Conjugation
Unconjugated
Epitope
Lys16
Preparation and Storage
Store at -20 degree C.
Immunohistochemistry (IHC)
(Human Skin: Formalin-Fixed, Paraffin-Embedded (FFPE))
Immunohistochemistry (IHC)
(Human Skin: Formalin-Fixed, Paraffin-Embedded (FFPE))
Immunohistochemistry (WB)
(Human Prostate: Formalin-Fixed, Paraffin-Embedded (FFPE))
Immunohistochemistry (WB)
(Human Prostate: Formalin-Fixed, Paraffin-Embedded (FFPE))
Immunofluorescence (IF)
(Immunofluorescence of HeLa cells, using Histone H4 (Acetyl-Lys16) Antibody. The sample on the right was incubated with synthetic peptide.)
Immunofluorescence (IF)
(Immunofluorescence of HeLa cells, using Histone H4 (Acetyl-Lys16) Antibody. The sample on the right was incubated with synthetic peptide.)
Related Product Information for anti-HIS4 antibody
main histone proteins involved in the structure of chromatin ineukaryotic cells. Featuring a main globular domain and a long N terminaltail, H4 is a structural component of the nucleosome, and is subject tocovalent modification, including acetylation and methylation, which mayalter expression of genes located on DNA associated with its parenthistone octamer.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
11,409 Da
NCBI Official Full Name
histone H4
NCBI Official Symbol
HIS4
NCBI Official Synonym Symbols
F8N16.2; F8N16_2; histone H4
NCBI Protein Information
histone H4
UniProt Protein Name
Histone H4
Protein Family
UniProt Gene Name
At1g07660
NCBI Description
histone 4
Uniprot Description
Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
Similar Products
Product Notes
The HIS4 at1g07660 (Catalog #AAA2401261) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-Histone H4 Antibody (Lys16) reacts with Human Predicted: Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Histone H4 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Immunofluorescence (IF), Immunohistochemistry (IHC) Paraffin. ELISA: 1:5000 IF: 1:100-1:500 IHC-P: 1:50. Researchers should empirically determine the suitability of the HIS4 at1g07660 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Histone H4, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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