Bcl-XL Active Protein | BCL2L1 active protein

Bcl-XL Protein, Mouse, Recombinant (aa 1-212, His Tag)

Cat. Num. AAA8122458

• Gene Names: Bcl2l1; BclX; Bcl2l; bcl-x; Bcl-XL; Bcl(X)L; bcl2-L-1
• Purity: >95% as determined by SDS-PAGE
• Synonyms: Bcl-XL; Bcl-XL Protein; Mouse; Recombinant (aa 1-212; His Tag); Mouse BCL2L1/Bcl-XL Protein (aa 1-212; Bcl(X)L Protein; bcl-x Protein; bcl2-L-1 Protein; Bcl2l Protein; BclX Protein; BCL2-like 1; BCL2L1 active protein

• Host: E Coli
• Purity/Purification: >95% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.5. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Arg212

• Species: Mouse
• Activity: 1. Measured by its binding ability in a functional ELISA.; 2. Immobilized human BID at 10ug/mL (100uL/well) can bind biotinylated mouse BCL2L1. The EC50 of biotinylated mouse BCL2L1 is 5.6ng/mL.; 3. Immobilized mouse BID at 10ug/mL (100uL/well) can bind biotinylated mouse BCL2L1. The EC50 of biotinylated mouse BCL2L1 is 7.1ng/mL.
• Predicted N Terminal: Met 1
• Tag: C-His
• Protein Construction: A DNA sequence encoding the mouse BCL2L1 (Q64373-1)(Met 1-Arg 212) was expressed, with a C-terminal polyhistidine tag.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Testing Data

(Measured by its binding ability in a functional ELISA. 1. Immobilized human BID at 10 ug/mL (100 ul/well) can bind biotinylated mouse BCL2L1, The EC50 of biotinylated mouse BCL2L1 is 5.6 ng/mL. 2. Immobilized mouse BID at 10 ug/mL (100 ul/well) can bind biotinylated mouse BCL2L1, The EC50 of biotinylated mouse BCL2L1 is 7.1 ng/mL.)

Related Product Information for BCL2L1 active protein

Background: B-cell lymphoma-extra large (Bcl-xl) is a transmembrane molecule in the mitochondria. Bcl-xL (BCL2L1), belongs to the Bcl-2 family. Members of the bcl-2 family encode proteins that function either to promote or to inhibit apoptosis. Antiapoptotic members such as Bcl-2 and Bcl-xL prevent PCD in response to a wide variety of stimuli to take part in cancer survival. Conversely, proapoptotic proteins, exemplified by Bax and Bak, can accelerate death and in some instances are sufficient to cause apoptosis independent of additional signals. The crystal and solution structures of a Bcl-2 family member, Bcl-xL is like this: The structures consist of two central, primarily hydrophobic alpha-helices, which are surrounded by amphipathic helices. A 60-residue loop connecting helices alphal and alpha2 was found to be flexible and non-essential for anti-apoptotic activity. Bcl-xL is characterized as an important factor in autophagy, inhibiting Beclin 1-mediated autophagy by binding to Beclin 1. In addition, Beclin 1, Bcl-2 and Bcl-xL can cooperate with Atg5 or Ca²⁺ to regulate both autophagy and apoptosis. Bcl-xL is also implicated in anoxia induced cell death. The pathway is initiated by the loss of function of the prosurvival Bcl-2 family members Mcl-1 and Bcl-2/Bcl-XL, resulting in Bax- or Bak-dependent release of cytochrome c and subsequent caspase-9-dependent cell death. Thus, Bcl-xL, the well-characterized apoptosis guards, appears to be important in cell death.

Product Categories/Family for BCL2L1 active protein

Death Receptor Signaling

References

Vander Heiden MG, et al. (1997) Bcl-xL Regulates the Membrane Potential and Volume Homeostasis of Mitochondria. Cell. 91 (5): 627-37.Muchmore SW, et al. (1996) X-ray and NMR structure of human Bcl-xL, an inhibitor of programmed cell death. Nature. 381: 335-341.SharoffEH, et al. (2007) Bcl-2 family members regulate anoxia-induced cell death. Antioxid Redox Signal. 9 (9) :1405-9.Zhou F, et al. (2011) Bcl-2 and Bcl-xL play important roles in the crosstalk between autophagy and apoptosis. FEBS J. 278 (3): 403-13.

NCBI and Uniprot Product Information

• NCBI GI #: 577019442
• NCBI GeneID: 12048
• NCBI Accession #: NP_001276645.1
• NCBI GenBank Nucleotide #: NM_001289716.1
• UniProt Accession #: Q64373; O35844; Q60657; Q60658; Q61338
• Molecular Weight: 23,900 Da
• NCBI Official Full Name: bcl-2-like protein 1 isoform a
• NCBI Official Synonym Full Names: BCL2-like 1
• NCBI Official Symbol: Bcl2l1
• NCBI Official Synonym Symbols: BclX; Bcl2l; bcl-x; Bcl-XL; Bcl(X)L; bcl2-L-1
• NCBI Protein Information: bcl-2-like protein 1
• UniProt Protein Name: Bcl-2-like protein 1
• Protein Family: Bcl-2-like protein
• UniProt Gene Name: Bcl2l1
• UniProt Synonym Gene Names: Bcl2l; Bclx; Bcl2-L-1
• UniProt Entry Name: B2CL1_MOUSE

Uniprot Description

Bcl-xL: an antiapoptotic member of the Bcl-2 family. Located at the outer mitochondrial membrane and regulates outer mitochondrial membrane channel (VDAC) opening. VDAC regulates mitochondrial membrane potential, and thus controls the production of reactive oxygen species and release of cytochrome C by mitochondria, both of which are potent inducers of cell apoptosis. Two alternatively spliced isoforms have been reported.

Protein type: Apoptosis; Autophagy; Membrane protein, integral; Mitochondrial

Cellular Component: centrosome; cytoplasm; cytosol; intracellular; mitochondrial envelope; mitochondrial membrane; mitochondrial outer membrane; mitochondrion

Molecular Function: BH domain binding; BH3 domain binding; caspase inhibitor activity; protein binding; protein heterodimerization activity; protein homodimerization activity; protein kinase binding; transcription factor binding

Biological Process: cell proliferation; cellular process regulating host cell cycle in response to virus; cytokinesis; DNA damage response, signal transduction resulting in induction of apoptosis; fertilization; germ cell development; growth; in utero embryonic development; male gonad development; mitotic cell cycle checkpoint; negative regulation of apoptosis; negative regulation of neuron apoptosis; neuron apoptosis; ovarian follicle development; positive regulation of apoptosis; positive regulation of cell proliferation; regulation of apoptosis; regulation of mitochondrial membrane permeability; regulation of mitochondrial membrane potential; release of cytochrome c from mitochondria; response to cycloheximide; response to cytokine stimulus; response to radiation; response to virus; spermatogenesis; suppression by virus of host apoptosis