Human C-type lectin domain family 9, member A ELISA Kit | CLEC9A elisa kit

Human C-type lectin domain family 9 member A, CLEC9A ELISA Kit

Cat. Num. AAA922282

• Gene Names: CLEC9A; DNGR1; UNQ9341
• Reactivity: Human
• Synonyms: C-type lectin domain family 9; member A; Human C-type lectin domain family 9 member A; CLEC9A ELISA Kit; Human C-type lectin domain family 9 member A (CLEC9A) ELISA kit; DNGR1; UNQ9341; HEEE9341; CLEC9A elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of human CLEC9A. No significant cross-reactivity or interference between human CLEC9A and analogues was observed.
• Sequence Length: 241

• Samples: Serum, plasma, tissue homogenates, Cell lysates
• Assay Type: Sandwich
• Detection Range: 0.312 ng/ml -20 ng/ml.
• Sensitivity: The minimum detectable dose of human CLEC9A is typically less than 0.078 ng/ml.The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
• Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CLEC9A elisa kit

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for CLEC9A has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CLEC9A present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CLEC9A is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CLEC9A bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 46409326
• NCBI GeneID: 283420
• NCBI Accession #: NP_997228.1
• NCBI GenBank Nucleotide #: NM_207345.2
• UniProt Accession #: Q6UXN8; B0ZBM2
• Molecular Weight: 27,324 Da
• NCBI Official Full Name: C-type lectin domain family 9 member A
• NCBI Official Synonym Full Names: C-type lectin domain family 9, member A
• NCBI Official Symbol: CLEC9A
• NCBI Official Synonym Symbols: DNGR1; UNQ9341
• NCBI Protein Information: C-type lectin domain family 9 member A; HEEE9341
• UniProt Protein Name: C-type lectin domain family 9 member A
• Protein Family: C-type lectin domain family
• UniProt Gene Name: CLEC9A
• UniProt Synonym Gene Names: UNQ9341/PRO34046
• UniProt Entry Name: CLC9A_HUMAN

NCBI Description

CLEC9A is a group V C-type lectin-like receptor (CTLR) that functions as an activation receptor and is expressed on myeloid lineage cells (Huysamen et al., 2008 [PubMed 18408006]).[supplied by OMIM, Aug 2008]

Uniprot Description

CLEC9A: Functions as an endocytic receptor on a small subset of myeloid cells and induces intracellular signaling via spleen tyrosine kinase (Syk). Acts as a receptor for necrotic cells and can mediate the cross-presentation of dead-cell associated antigens in a Syk-dependent manner.

Protein type: Membrane protein, integral; Receptor, misc.

Chromosomal Location of Human Ortholog: 12p13.2

Cellular Component: cell surface; integral to membrane

Molecular Function: carbohydrate binding

Biological Process: receptor-mediated endocytosis; positive regulation of cytokine secretion