Human coagulation factor VII (serum prothrombin conversion accelerator) ELISA Kit | F7 elisa kit

Human coagulation factor VII, FVII ELISA Kit

Cat. Num. AAA700117

• Gene Names: F7; SPCA
• Reactivity: Human
• Synonyms: coagulation factor VII (serum prothrombin conversion accelerator); Human coagulation factor VII; FVII ELISA Kit; FVII coagulation protein; coagulation factor VII; eptacog alfa; F7 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of human FVII. No significant cross-reactivity or interference between human FVII and analogues was observed.
• Sequence Length: 466

• Samples: Serum, plasma, tissue homogenates
• Assay Type: Sandwich
• Detection Range: 0.312 ng/ml-20 ng/ml.
• Sensitivity: The minimum detectable dose of human FVII is typically less than 0.078 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
• Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for F7 elisa kit

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for FVII has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any FVII present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for FVII is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of FVII bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 4503645
• NCBI GeneID: 2155
• NCBI Accession #: NP_000122.1
• NCBI GenBank Nucleotide #: NM_000131.4
• UniProt Accession #: P08709; Q14339; Q5JVF1; Q5JVF2; Q9UD52; Q9UD53; Q9UD54; B0YJC8
• Molecular Weight: 51,594 Da
• NCBI Official Full Name: coagulation factor VII isoform a preproprotein
• NCBI Official Synonym Full Names: coagulation factor VII (serum prothrombin conversion accelerator)
• NCBI Official Symbol: F7
• NCBI Official Synonym Symbols: SPCA
• NCBI Protein Information: coagulation factor VII; eptacog alfa; proconvertin; FVII coagulation protein
• UniProt Protein Name: Coagulation factor VII
• Protein Family: Coagulation factor
• UniProt Gene Name: F7
• UniProt Synonym Gene Names: SPCA
• UniProt Entry Name: FA7_HUMAN

NCBI Description

This gene encodes coagulation factor VII which is a vitamin K-dependent factor essential for hemostasis. This factor circulates in the blood in a zymogen form, and is converted to an active form by either factor IXa, factor Xa, factor XIIa, or thrombin by minor proteolysis. Upon activation of the factor VII, a heavy chain containing a catalytic domain and a light chain containing 2 EGF-like domains are generated, and two chains are held together by a disulfide bond. In the presence of factor III and calcium ions, the activated factor then further activates the coagulation cascade by converting factor IX to factor IXa and/or factor X to factor Xa. Defects in this gene can cause coagulopathy. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, May 2012]

Uniprot Description

Function: Initiates the extrinsic pathway of blood coagulation. Serine protease that circulates in the blood in a zymogen form. Factor VII is converted to factor VIIa by factor Xa, factor XIIa, factor IXa, or thrombin by minor proteolysis. In the presence of tissue factor and calcium ions, factor VIIa then converts factor X to factor Xa by limited proteolysis. Factor VIIa will also convert factor IX to factor IXa in the presence of tissue factor and calcium.

Catalytic activity: Selective cleavage of Arg-|-Ile bond in factor X to form factor Xa.