Human resistin ELISA Kit | RETN elisa kit

human Resistin ELISA Kit

Cat. Num. AAA700822

• Gene Names: RETN; ADSF; RSTN; XCP1; FIZZ3; RETN1
• Reactivity: Human
• Synonyms: resistin; human Resistin ELISA Kit; human Resistin ELISA KIT; ADSF; FIZZ3; MGC126603; MGC126609; RETN1; RSTN; XCP1; C/EBP-epsilon regulated myeloid-specific secreted cysteine-rich protein precursor 1; found in inflammatory zone 3; RETN elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of human resistin. No significant cross-reactivity or interference between human resistin and analogues was observed.
• Sequence Length: 108

• Samples: Serum, plasma and tissue homogenates
• Assay Type: Sandwich
• Detection Range: 0.312 ng/ml -20 ng/ml.
• Sensitivity: The minimum detectable dose of human resistin is typically less than 0.078 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
• Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for RETN elisa kit

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for resistin has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any resistin present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for resistin is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of resistin bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 301129180
• NCBI GeneID: 56729
• NCBI Accession #: NP_001180303.1
• NCBI GenBank Nucleotide #: NM_001193374.1
• UniProt Accession #: Q9HD89; Q540D9; Q76B53; D6W649
• Molecular Weight: 11,419 Da
• NCBI Official Full Name: resistin
• NCBI Official Synonym Full Names: resistin
• NCBI Official Symbol: RETN
• NCBI Official Synonym Symbols: ADSF; RSTN; XCP1; FIZZ3; RETN1
• NCBI Protein Information: resistin; resistin delta2; found in inflammatory zone 3; cysteine-rich secreted protein FIZZ3; adipose tissue-specific secretory factor; cysteine-rich secreted protein A12-alpha-like 2; c/EBP-epsilon-regulated myeloid-specific secreted cysteine-rich protein; C/EBP-epsilon regulated myeloid-specific secreted cysteine-rich protein precursor 1
• UniProt Protein Name: Resistin
• Protein Family: Resistin
• UniProt Gene Name: RETN
• UniProt Synonym Gene Names: FIZZ3; HXCP1; RSTN; UNQ407/PRO1199; ADSF
• UniProt Entry Name: RETN_HUMAN

NCBI Description

This gene belongs to the family defined by the mouse resistin-like genes. The characteristic feature of this family is the C-terminal stretch of 10 cys residues with identical spacing. The mouse homolog of this protein is secreted by adipocytes, and may be the hormone potentially linking obesity to type II diabetes. Alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2010]

Uniprot Description

Function: Hormone that seems to suppress insulin ability to stimulate glucose uptake into adipose cells. Potentially links obesity to diabetes.