BCAM recombinant protein
Recombinant Rat BCAM Protein (His tag)
Gene Names
Bcam; Lu
Purity
> 90 % as determined by SDS-PAGE
Synonyms
BCAM; Recombinant Rat BCAM Protein (His tag); BCAM recombinant protein
Host
Human Cells
Purity/Purification
> 90 % as determined by SDS-PAGE
Form/Format
Lyophilized from sterile PBS, pH 7.4
Sequence Length
624
Application Notes
The recombinant rat BCAM comprises 529 amino acids and predicts a molecular mass of 58.2 kDa. The apparent molecular mass of the recombinant protein is approximately 68 kDa in SDS-PAGE under reducing conditions due to glycosylation.
Predicted N Terminal
Glu 26
Endotoxin
< 1.0 EU per mug of the protein as determined by the LAL method
Preparation and Storage
Samples are stable for up to twelve months from date of receipt at -70 degree C
SDS-PAGE
Related Product Information for BCAM recombinant protein
Background: The Lutheran (Lu) blood group and basal cell adhesion molecule (BCAM) antigens are both carried by 2 glycoprotein isoforms of the immunoglobulin superfamily representing receptors for the laminin alpha (5) chain. It is a transmembrane receptor with five immunoglobulin-like domains in its extracellular region, and is therefore classified as a member of the immunoglobulin (Ig) gene family. In addition to red blood cells, Lu/BCAM proteins are expressed in endothelial cells of vascular capillaries and in epithelial cells of several tissues. BCAM/LU has a wide tissue distribution with a predominant expression in the basal layer of the epithelium and the endothelium of blood vessel walls. As designated as CD239 recently, BCAM and LU share a significant sequence similarity with the CD146 (MUC18) and CD166, and themselves are adhesion molecules that bind laminin with high affinity. Laminins are found in all basement membranes and are involved in cell differentiation, adhesion, migration, and proliferation. BCAM is upregulated following malignant transformation of some cell type s in vivo and in vitro, thus being a candidate molecule involved in tumor progression. In addition, BCAM interacts with integrin in sickle red cells, and thus may potentially play a role in vaso-occlusive episodes.
Description: A DNA sequence encoding the rat BCAM (Q9ESS6) (Met1-Ala543) was expressed, fused with a polyhistidine tag at the C-terminus.
Description: A DNA sequence encoding the rat BCAM (Q9ESS6) (Met1-Ala543) was expressed, fused with a polyhistidine tag at the C-terminus.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
67,512 Da
NCBI Official Full Name
basal cell adhesion molecule
NCBI Official Synonym Full Names
basal cell adhesion molecule (Lutheran blood group)
NCBI Official Symbol
Bcam
NCBI Official Synonym Symbols
Lu
NCBI Protein Information
basal cell adhesion molecule
UniProt Protein Name
Basal cell adhesion molecule
Protein Family
UniProt Gene Name
Bcam
UniProt Synonym Gene Names
Lu
NCBI Description
may act as an adhesion molecule and receptor for laminin [RGD, Feb 2006]
Uniprot Description
Lu: Laminin alpha-5 receptor. May mediate intracellular signaling.
Protein type: Membrane protein, integral; Receptor, misc.
Chromosomal Location of Human Ortholog: 1q21
Cellular Component: cell surface; plasma membrane
Molecular Function: laminin binding; laminin receptor activity; protein C-terminus binding
Biological Process: cell adhesion; cell-matrix adhesion
