Testing Data
Norovirus GII full length capsid Recombinant Protein
Human Norovirus GII full length capsid
Applications
ELISA, Western Blot
Purity
>85% (12% SDS-PAGE, Coomassie stain). Proprietary chromatographic technique
Synonyms
Norovirus GII full length capsid; Human Norovirus GII full length capsid; Norovirus GII full length capsid recombinant protein
Host
E Coli
Specificity
>85% (12% SDS-PAGE, Coomassie stain). Proprietary chromatographic technique
Purity/Purification
>85% (12% SDS-PAGE, Coomassie stain). Proprietary chromatographic technique
Form/Format
Purified, Liquid. PBS with 1M urea and 50mM arginine
Concentration
1.4mg/ml (lot 04-17-2017) (varies by lot)
Applicable Applications for Norovirus GII full length capsid recombinant protein
ELISA (EIA), Western Blot (WB), Vaccine Study, Immunoassay (IA)
Species
Human
Important Note
Centrifuge before opening to ensure complete recovery of vial contents.
Preparation and Storage
Upon receipt, store at -20 degree C. Avoid multiple freeze/thaw cycles
Testing Data
Testing Data
Related Product Information for Norovirus GII full length capsid recombinant protein
Human Norovirus has two groups, group 1 and group 2. This group 2 recombinant capsid was derived from the full length capsid 53 to 548 amino acids (Strain: Hu/Beijing/06/2005/CHN, Swiss-Prot B5AHE9)
The full length capsid contains two regions, S and P domains. N-terminal 225 amino acids is an essential element forming icosahedron.
Full length GII capsid protein was expressed and purified from E Coli with a 6 x his tag at its C-terminus, this recombinant protein forms virus like particle (VLP), showing multiple bands on SDS-PAGE in non-reduced condition, after reduction, the recombinant capsid protein changes to monomer status.
Except the full length capsid protein, C-terminal P1-P2-P protein from GII VA387 strain will become available soon. C terminal capsid plays more important role in viral function, such as viral attachement to intestinal epithelial cell and immunal reaction with the specific antibody. Receptor binding region to human histo-blood group antigens (HBGAs) and important immunal epitopes directly reacting to the specific antibody are located within capsid C-terminal. P domain expressed in bacteria can spontaneously form P dime and P particle aggregated by 12 P dimmers. P particle displays an enhanced binding ability to HBGAs compared to virus-like particle (VLP) formed by the full length capsid as well as better immunal reaction.
The full length capsid contains two regions, S and P domains. N-terminal 225 amino acids is an essential element forming icosahedron.
Full length GII capsid protein was expressed and purified from E Coli with a 6 x his tag at its C-terminus, this recombinant protein forms virus like particle (VLP), showing multiple bands on SDS-PAGE in non-reduced condition, after reduction, the recombinant capsid protein changes to monomer status.
Except the full length capsid protein, C-terminal P1-P2-P protein from GII VA387 strain will become available soon. C terminal capsid plays more important role in viral function, such as viral attachement to intestinal epithelial cell and immunal reaction with the specific antibody. Receptor binding region to human histo-blood group antigens (HBGAs) and important immunal epitopes directly reacting to the specific antibody are located within capsid C-terminal. P domain expressed in bacteria can spontaneously form P dime and P particle aggregated by 12 P dimmers. P particle displays an enhanced binding ability to HBGAs compared to virus-like particle (VLP) formed by the full length capsid as well as better immunal reaction.
Product Categories/Family for Norovirus GII full length capsid recombinant protein
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Product Notes
The Norovirus GII full length capsid (Catalog #AAA596164) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Norovirus GII full length capsid can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Vaccine Study, Immunoassay (IA). Researchers should empirically determine the suitability of the Norovirus GII full length capsid for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Norovirus GII full length capsid, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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