BCAM active protein

BCAM Protein, Rat, Recombinant (His Tag)

Cat. Num. AAA8123386

• Gene Names: Bcam; Lu
• Purity: >90% as determined by SDS-PAGE
• Synonyms: BCAM; BCAM Protein; Rat; Recombinant (His Tag); Rat BCAM Protein (His Tag); basal cell adhesion molecule (Lutheran blood group); BCAM active protein

• Host: HEK293 Cells
• Purity/Purification: >90% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Ala543

• Species: Rat
• Activity: Measured by the ability of the immobilized protein to support the adhesion of HOS human osteogenic sarcoma cells. When cells are added to coated plates(10ug/mL,100uL/well), approximately 20-50% will adhere after 1 hour at 37 degree C.
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Glu 26
• Tag: C-His
• Protein Construction: A DNA sequence encoding the rat BCAM (Q9ESS6)(Met1-Ala543) was expressed, fused with a polyhistidine tag at the C-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for BCAM active protein

Background: The Lutheran (Lu) blood group and basal cell adhesion molecule (BCAM) antigens are both carried by 2 glycoprotein isoforms of the immunoglobulin superfamily representing receptors for the laminin alpha(5) chain. It is a transmembrane receptor with five immunoglobulin-like domains in its extracellular region, and is therefore classified as a member of the immunoglobulin (Ig) gene family. In addition to red blood cells, Lu/BCAM proteins are expressed in endothelial cells of vascular capillaries and in epithelial cells of several tissues. BCAM/LU has a wide tissue distribution with a predominant expression in the basal layer of the epithelium and the endothelium of blood vessel walls. As designated as CD239 recently, BCAM and LU share a significant sequence similarity with the CD146 (MUC18) and CD166, and themselves are adhesion molecules that bind laminin with high affinity. Laminins are found in all basement membranes and are involved in cell differentiation, adhesion, migration, and proliferation. BCAM is upregulated following malignant transformation of some cell types in vivo and in vitro, thus being a candidate molecule involved in tumor progression. In addition, BCAM interacts with integrin in sickle red cells, and thus may potentially play a role in vaso-occlusive episodes.

References

Kikkawa Y, et al. (2005) Review: Lutheran/B-CAM: a laminin receptor on red blood cells and in various tissues. Connect Tissue Res. 46 (4-5): 193-9.El Nemer W, et al. (2007) Endothelial Lu/BCAM glycoproteins are novel ligands for red blood cell alpha4beta1 integrin: role in adhesion of sickle red blood cells to endothelial cells. Blood. 109 (8): 3544-51.Colin Y, et al. (2008) Red cell and endothelial Lu/BCAM beyond sickle cell disease. Transfus Clin Biol. 15 (6): 402-5.El Nemer W, et al. (2008) Role of Lu/BCAM in abnormal adhesion of sickle red blood cells to vascular endothelium. Transfus Clin Biol. 15 (1-2): 29-33.

NCBI and Uniprot Product Information

• NCBI GI #: 13929056
• NCBI GeneID: 78958
• NCBI Accession #: NP_113940.1
• NCBI GenBank Nucleotide #: NM_031752.2
• UniProt Accession #: Q9ESS6
• Molecular Weight: 67,512 Da
• NCBI Official Full Name: basal cell adhesion molecule
• NCBI Official Synonym Full Names: basal cell adhesion molecule (Lutheran blood group)
• NCBI Official Symbol: Bcam
• NCBI Official Synonym Symbols: Lu
• NCBI Protein Information: basal cell adhesion molecule
• UniProt Protein Name: Basal cell adhesion molecule
• Protein Family: Basal cell adhesion molecule
• UniProt Gene Name: Bcam
• UniProt Synonym Gene Names: Lu
• UniProt Entry Name: BCAM_RAT

NCBI Description

may act as an adhesion molecule and receptor for laminin [RGD, Feb 2006]

Uniprot Description

Lu: Laminin alpha-5 receptor. May mediate intracellular signaling.

Protein type: Receptor, misc.; Membrane protein, integral; Cell surface; Cell adhesion

Cellular Component: cell surface; integral to membrane; plasma membrane

Molecular Function: laminin binding; laminin receptor activity; protein C-terminus binding

Biological Process: cell adhesion; cell-matrix adhesion