MKK4 Cell Lysate | MAP2K4 cell lysate
Mouse MKK4 Baculovirus-Insect cells Overexpression Lysate
Applications
Western Blot
Synonyms
MKK4; Mouse MKK4 Baculovirus-Insect cells Overexpression Lysate; Mouse MKK4/MEK4/MAP2K4 Insect Cell Lysate (WB positive control); mitogen-activated protein kinase kinase 4; Mouse JNKK1 Overexpression Lysate; Mouse MEK4 Overexpression Lysate; Mouse MKK4 Overexpression Lysate; Mouse PRKMK4 Overexpression Lysate; Mouse Sek1 Overexpression Lysate; Mouse Serk1 Overexpression Lysate; MAP2K4 cell lysate
Host
Baculovirus-Insect cells
Form/Format
1x Sample Buffer (1x modified RIPA buffer+1x SDS loading buffer).
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Sequence Positions
633aa
Sequence
Ala2-Asp397
Applicable Applications for MAP2K4 cell lysate
Western Blot (WB)
Application Notes
WB: Use at an assay dependent dilution.
Species
Mouse
Recommend Usage
1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200uL pure water and boil for 2-5 min.
Sequence Construction
A DNA sequence encoding the mouse MAP2K4 (P47809) (Ala 2-Asp 397) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.
Preparation Method
Cell lysate was prepared by homogenization of the over-expressed cells in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Preparation and Storage
Store at 4 degree C for up to twelve months from date of receipt. After re-dissolution, aliquot and store at -80 degree C for up to twelve months. Avoid repeated freeze-thaw cycles.
Samples are stable for up to twelve months from date of receipt.
Samples are stable for up to twelve months from date of receipt.
Related Product Information for MAP2K4 cell lysate
Dual specificity mitogen-activated protein kinase kinase 4, also known as MAP kinase kinase 4, MAPKK4, JNK-activating kinase 1, MAPK/ERK kinase 4, SAPK/ERK kinase 1, c-Jun N-terminal kinase kinase 1, JNKK, and MAP2K4, is a protein that belongs to the protein kinase superfamily, STE Ser/Thr protein kinase family and MAP kinase kinase subfamily. MAP2K4/JNKK1 is a protein kinase that is a direct activator of MAP kinases in response to various environmental stresses or mitogenic stimuli. MAP2K4/JNKK1 has been shown to activate MAPK8/JNK1, MAPK9/JNK2, and MAPK14/p38, but not MAPK1/ERK2 or MAPK3/ERK1. MAP2K4/JNKK1 is phosphorylated, and thus activated by MAP3K1/MEKK. The stress-activated protein kinase (SAPK) pathways represent phosphorylation cascades that convey pro-apoptotic signals. The mitogen-activated protein kinase kinase (MAPKK) homolog MAP2K4 (MKK4, SEK, JNKK1) is a centrally-placed mediator of the SAPK pathways.
This Mouse MKK4 overexpression lysate was created in Baculovirus-Insect cells and intented for use as a Western blot (WB) positive control. Purification of MKK4 protein from the overexpression lysate was verified.
This Mouse MKK4 overexpression lysate was created in Baculovirus-Insect cells and intented for use as a Western blot (WB) positive control. Purification of MKK4 protein from the overexpression lysate was verified.
Product Categories/Family for MAP2K4 cell lysate
References
Lin A, et al., 1995, Science 268 (5208): 286-90. Su, G.H. et al., 2002, Hum Mutat. 19 (1):81. Lee, et al., 2002, Proc. Natl. Acad. Sci. USA. 99 (22): 14189-94. Bouzakri, K. et al., 2007, J Biol Chem. 282 (11):7783-9.
NCBI and Uniprot Product Information
UniProt Accession #
Molecular Weight
The secreted recombinant mouse MAP2K4/GST chimera consists of 633 amino acids and has a calculated molecular mass of 72kDa.
The recombinant protein migrates as an approximately 65kDa band in SDS-PAGE under reducing conditions.
The recombinant protein migrates as an approximately 65kDa band in SDS-PAGE under reducing conditions.
UniProt Protein Name
Dual specificity mitogen-activated protein kinase kinase 4
Protein Family
UniProt Gene Name
Map2k4
UniProt Synonym Gene Names
Jnkk1; Prkmk4; Sek1; Serk1
UniProt Entry Name
MP2K4_MOUSE
Uniprot Description
MKK4: dual specificity kinase of the STE7 family that phosphorylates and activates JNK1 and -2 as well as p38 but not ERK1 or -2. Mediates cellular responses to various cellular stresses and inflammatory cytokines. Phosphorylation by Akt inhibits MKK4 and suppresses stress-activated signal transduction.
Protein type: Protein kinase, dual-specificity (non-receptor); Kinase, protein; Protein kinase, STE; EC 2.7.12.2; STE group; STE7 family
Cellular Component: dendrite cytoplasm; axon; cytoplasm; perikaryon; intracellular; cytosol; nucleus
Molecular Function: transferase activity; MAP kinase kinase activity; protein serine/threonine kinase activity; protein binding; protein-tyrosine kinase activity; transferase activity, transferring phosphorus-containing groups; nucleotide binding; kinase activity; mitogen-activated protein kinase kinase kinase binding; JUN kinase kinase activity; ATP binding; protein kinase activity
Biological Process: apoptosis; positive regulation of apoptosis; positive regulation of neuron apoptosis; MAPKKK cascade; JNK cascade; positive regulation of protein amino acid phosphorylation; protein amino acid phosphorylation; phosphorylation; positive regulation of DNA replication; activation of JNK activity; positive regulation of nitric-oxide synthase biosynthetic process
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Product Notes
The MAP2K4 map2k4 (Catalog #AAA8116759) is a Cell Lysate produced from Baculovirus-Insect cells and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 633aa. AAA Biotech's MKK4 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: Use at an assay dependent dilution. Researchers should empirically determine the suitability of the MAP2K4 map2k4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Ala2-Asp39 7. It is sometimes possible for the material contained within the vial of "MKK4, Cell Lysate" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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