Hamster IL-10 (Interleukin-10) ELISA Kit | IL-10 elisa kit

Hamster IL-10 (Interleukin-10) ELISA Kit

Cat. Num. AAA7606616

• Gene Names: IL10; CSIF; TGIF; GVHDS; IL-10; IL10A
• Reactivity: Hamster
• Synonyms: IL-10 (Interleukin-10); Hamster IL-10 (Interleukin-10) ELISA Kit; IL-10 elisa kit

• Reactivity: Hamster
• Specificity: This assay has high sensitivity and excellent specificity for detection of IL-10. No significant cross-reactivity or interference between IL-10 and analogues was observed.
• Sequence Length: 178

• Samples: Serum, plasma, tissue homogenates and other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 6.25-400pg/ml
• Sensitivity: 3.75pg/ml
• Intra-assay Precision: CV<8%
• Inter-assay Precision: CV<10%
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data/Standard Curve (for reference only)

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Related Product Information for IL-10 elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Capture antibody was pre-coated onto 96-well plates. And the biotin conjugated antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of target can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 10835141
• NCBI GeneID: 3586
• NCBI Accession #: NP_000563.1
• NCBI GenBank Nucleotide #: NM_000572.2
• Molecular Weight: 20,517 Da
• NCBI Official Full Name: interleukin-10
• NCBI Official Synonym Full Names: interleukin 10
• NCBI Official Symbol: IL10
• NCBI Official Synonym Symbols: CSIF; TGIF; GVHDS; IL-10; IL10A
• NCBI Protein Information: interleukin-10
• UniProt Protein Name: Interleukin-10
• UniProt Gene Name: IL10
• UniProt Synonym Gene Names: IL-10; CSIF

NCBI Description

The protein encoded by this gene is a cytokine produced primarily by monocytes and to a lesser extent by lymphocytes. This cytokine has pleiotropic effects in immunoregulation and inflammation. It down-regulates the expression of Th1 cytokines, MHC class II Ags, and costimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. This cytokine can block NF-kappa B activity, and is involved in the regulation of the JAK-STAT signaling pathway. Knockout studies in mice suggested the function of this cytokine as an essential immunoregulator in the intestinal tract. Mutations in this gene are associated with an increased susceptibility to HIV-1 infection and rheumatoid arthritis.[provided by RefSeq, May 2011]

Uniprot Description

IL10: Inhibits the synthesis of a number of cytokines, including IFN-gamma, IL-2, IL-3, TNF and GM-CSF produced by activated macrophages and by helper T-cells. Belongs to the IL-10 family.

Protein type: Cytokine; Motility/polarity/chemotaxis; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 1q32.1

Cellular Component: extracellular region; extracellular space

Molecular Function: protein binding

Biological Process: cell-cell signaling; hemopoiesis; inflammatory response; leukocyte chemotaxis; negative regulation of B cell proliferation; negative regulation of cell proliferation; negative regulation of cytokine secretion during immune response; negative regulation of heterotypic cell-cell adhesion; negative regulation of interleukin-1 production; negative regulation of interleukin-12 production; negative regulation of interleukin-18 production; negative regulation of interleukin-6 production; negative regulation of interleukin-8 production; negative regulation of membrane protein ectodomain proteolysis; negative regulation of MHC class II biosynthetic process; negative regulation of mitotic cell cycle; negative regulation of tumor necrosis factor production; positive regulation of B cell apoptosis; positive regulation of cytokine secretion; positive regulation of heterotypic cell-cell adhesion; positive regulation of transcription factor activity; positive regulation of transcription, DNA-dependent; receptor biosynthetic process; regulation of gene expression; response to glucocorticoid stimulus; response to molecule of bacterial origin; T-helper 2 type immune response

Disease: Graft-versus-host Disease, Susceptibility To; Human Immunodeficiency Virus Type 1, Susceptibility To; Rheumatoid Arthritis