PPIL1 recombinant protein

PPIL1 Protein, Human, Recombinant (His Tag)

Cat. Num. AAA8121634

• Gene Names: PPIL1; CYPL1; hCyPX; PPIase; CGI-124
• Purity: >90% as determined by SDS-PAGE
• Synonyms: PPIL1; PPIL1 Protein; Human; Recombinant (His Tag); Human PPIL1 Protein (His Tag); CGI-124 Protein; CYPL1 Protein; hCyPX Protein; PPIase Protein; peptidylprolyl isomerase (cyclophilin)-like 1; PPIL1 recombinant protein

• Host: E Coli
• Purity/Purification: >90% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile 50mM Tris, 10% glycerol, pH8.0. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Gly166

• Species: Human
• Predicted N Terminal: Met
• Tag: C-His
• Protein Construction: A DNA sequence encoding the human PPIL1 (Q9Y3C6)(Met1-Gly166) was expressed with a polyhistidine tag at the C-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for PPIL1 recombinant protein

Background: PPIL1 is a member of the cyclophilin family. Cyclophilins are well conserved and ubiquitous. Members of cyclophilin family take a significant part in protein folding, immunosuppression by cyclosporin A, and infection of HIV-1 virions. PPIL1 is a peptidylprolyl isomerase(PPIase). It increases the folding of proteins and catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. PPIL1 is involved in proliferation of cancer cells through modulation of phosphorylation of stathmin. It is a novel molecular target for colon-cancer therapy.

References

Xu C. et al., 2005, J Biomol NMR. 31 (2): 179-80. Ozaki K. et al., 1997, Cytogenet Cell Genet. 72 (2-3): 242-5. Mann SS. et al., 1999, Cytogenet Cell Genet. 83 (3-4): 228-9.

NCBI and Uniprot Product Information

• NCBI GI #: 7706339
• NCBI GeneID: 51645
• NCBI Accession #: NP_057143.1
• NCBI GenBank Nucleotide #: NM_016059.4
• UniProt Accession #: Q9Y3C6; O15001; Q5TDC9
• Molecular Weight: 18,237 Da
• NCBI Official Full Name: peptidyl-prolyl cis-trans isomerase-like 1
• NCBI Official Synonym Full Names: peptidylprolyl isomerase (cyclophilin)-like 1
• NCBI Official Symbol: PPIL1
• NCBI Official Synonym Symbols: CYPL1; hCyPX; PPIase; CGI-124
• NCBI Protein Information: peptidyl-prolyl cis-trans isomerase-like 1; cyclophilin-related gene 1; rotamase PPIL1
• UniProt Protein Name: Peptidyl-prolyl cis-trans isomerase-like 1
• Protein Family: Peptidyl-prolyl cis-trans isomerase
• UniProt Gene Name: PPIL1
• UniProt Synonym Gene Names: CYPL1; PPIase
• UniProt Entry Name: PPIL1_HUMAN

NCBI Description

This gene is a member of the cyclophilin family of peptidylprolyl isomerases (PPIases). The cyclophilins are a highly conserved, ubiquitous family, members of which play an important role in protein folding, immunosuppression by cyclosporin A, and infection of HIV-1 virions. Based on similarity to other PPIases, this protein could accelerate the folding of proteins and might catalyze the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. [provided by RefSeq, Jul 2008]

Uniprot Description

PPIL1: PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. May be involved in pre-mRNA splicing. Belongs to the cyclophilin-type PPIase family. PPIL1 subfamily.

Protein type: Cyclophilin; Isomerase; EC 5.2.1.8; Spliceosome

Chromosomal Location of Human Ortholog: 6p21.1

Molecular Function: protein binding; peptidyl-prolyl cis-trans isomerase activity

Biological Process: nuclear mRNA splicing, via spliceosome; protein peptidyl-prolyl isomerization; protein folding