Rabbit Chemerin ELISA Kit | CHE elisa kit

Rabbit Chemerin ELISA Kit

Cat. Num. AAA730164

• Reactivity: Rabbit
• Synonyms: Chemerin; Rabbit Chemerin ELISA Kit; CHE elisa kit

• Reactivity: Rabbit

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Sandwich
• Detection Range: 50-1000pg/mL?
• Sensitivity: 1.0pg/mL
• Intended Uses: This CHE ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rabbit CHE. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CHE elisa kit

Principle of the Assay: CHE ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-CHE antibody and an CHE-HRP conjugate. The assay sample and buffer are incubated together with CHE-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CHE concentration since CHE from samples and CHE-HRP conjugate compete for the anti-CHE antibody binding site. Since the number of sites is limited, as more sites are occupied by CHE from the sample, fewer sites are left to bind CHE-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CHE concentration in each sample is interpolated from this standard curve.

Product Categories/Family for CHE elisa kit

Signal Transduction

NCBI and Uniprot Product Information

• NCBI GI #: 49457005
• UniProt Accession #: Q7LE02
• Molecular Weight: 18,618 Da
• NCBI Official Full Name: RARRES2, partial
• UniProt Protein Name: Retinoic acid receptor responder protein 2
• UniProt Gene Name: RARRES2
• UniProt Synonym Gene Names: TIG2
• UniProt Entry Name: RARR2_HUMAN

Uniprot Description

RARRES2: Inhibited in psoriatic lesions. Activated by tazarotene in skin rafts and in the epidermis of psoriatic lesions.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 7q36.1

Cellular Component: extracellular matrix; extracellular region

Molecular Function: protein binding; receptor binding

Biological Process: in utero embryonic development; positive regulation of chemotaxis; brown fat cell differentiation; positive regulation of fat cell differentiation; positive regulation of protein amino acid phosphorylation; chemotaxis; inflammatory response; retinoid metabolic process; embryonic gut development; regulation of lipid catabolic process